A5085708867- Adipose Tissue and Metabolism
- Mesenchymal stem cell research
- Adipokines, Inflammation, and Metabolic Diseases
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Muscle metabolism and nutrition
- Regulation of Appetite and Obesity
- Cardiovascular Disease and Adiposity
- Fatty Acid Research and Health
- Exercise and Physiological Responses
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- Osteoarthritis Treatment and Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- Body Contouring and Surgery
- Angiogenesis and VEGF in Cancer
- Periodontal Regeneration and Treatments
- Cellular Mechanics and Interactions
- Antioxidant Activity and Oxidative Stress
- Immune Cell Function and Interaction
- Wound Healing and Treatments
Centre National de la Recherche Scientifique
2015-2024
Université de Toulouse
2015-2024
Inserm
2015-2024
RESTORE
2021-2024
École Nationale Vétérinaire de Toulouse
2016-2024
Établissement Français du Sang
2012-2024
Institute for Regenerative Medicine & Biotherapy
2024
Université Toulouse III - Paul Sabatier
2012-2021
StromaLab
2010-2020
Canadian Nautical Research Society
2020
Diabetes and obesity are two metabolic diseases characterized by insulin resistance a low-grade inflammation. Seeking an inflammatory factor causative of the onset resistance, obesity, diabetes, we have identified bacterial lipopolysaccharide (LPS) as triggering factor. We found that normal endotoxemia increased or decreased during fed fasted state, respectively, on nutritional basis 4-week high-fat diet chronically plasma LPS concentration to three times, threshold defined endotoxemia....
Background— Adipose tissue development and remodeling are closely associated with the growth of vascular network. We hypothesized that adipose may contain progenitor cells angiogenic potential therapy based on tissue-derived administration constitute a promising cell in patients ischemic disease. Methods Results— In mice, cultured stromal-vascular fraction (SVF) from have great proangiogenic potential, comparable to bone marrow mononuclear mouse hindlimb model. Similarly, human SVF...
Summary Like mesenchymal stem cells from bone marrow (BM‐MSCs), adipose tissue‐derived adult (ADAS cells) can differentiate into several lineages and present therapeutical potential for repairing damaged tissues. The use of allogenic enlarge their interest, provided that the grafted could be tolerated. We investigate here, first time, immunosuppressive properties ADAS compared with well‐characterized BM‐MSCs. did not provoke in vitro alloreactivity incompatible lymphocytes and, moreover,...
According to the state of mitochondrial respiration, respiratory chain generates superoxide anions converted into hydrogen peroxide. Two uncoupling proteins (UCP) able modulate coupling between and ATP synthesis are now identified could be involved in H2O2 generation. UCP1 is specific brown adipose tissue (BAT) whereas UCP2 expressed numerous tissues, particularly monocytes/macrophages. Preincubation BAT fractions with GDP, an inhibitor UCP1, induced a rise membrane potential (assessed by...
Cardiomyocyte regeneration is limited in adult life. Thus, the identification of a putative source cardiomyocyte progenitors great interest to provide usable model vitro and new perspective regenerative therapy. As adipose tissues were recently demonstrated contain pluripotent stem cells, emergence phenotype from adipose-derived cells was investigated. We that rare beating with features could be identified after culture stroma without addition 5-azacytidine. The first by morphological...
ABSTRACT Brown adipocytes are thermogenic cells which play an important role in energy balance. Their activity is due to the presence of a mitochondrial uncoupling protein (UCP). Until recently, it was admitted that rodents brown were mainly located classical adipose tissue (BAT). In present study, we have investigated UCP or mRNA white (WAT) rats. Using polymerase chain reaction Northern blot hybridization, detected mesenteric, epidydimal, retroperitoneal, inguinal and particularly...
Preadipocytes are present throughout adult life in adipose tissues and can proliferate differentiate into mature adipocytes according to the energy balance. An increasing number of reports demonstrate that cells from lineages (preadipocytes adipocytes) macrophages share numerous functional or antigenic properties. No large scale comparison reflecting phenotype complexity has been performed between these different cell types until now. We used profiling analysis define common features shared...
: Osteoarthritis (OA) is the most widespread musculoskeletal disorder in adults. It leads to cartilage damage associated with subchondral bone changes and synovial inflammation, causing pain disability. The present study aimed at evaluating safety of a dose-escalation protocol intra-articular injected adipose-derived stromal cells (ASCs) patients knee OA, as well clinical efficacy secondary endpoint. A bicentric, uncontrolled, open phase I trial was conducted France Germany regulatory agency...
OBJECTIVE—Insulin secretion involves complex events in which the mitochondria play a pivotal role generation of signals that couple glucose detection to insulin secretion. Studies on mitochondrial reactive oxygen species (ROS) generally focus chronic nutrient exposure. Here, we investigate whether transient ROS production linked glucose-induced increased respiration might act as signal for monitoring RESEARCH DESIGN AND METHODS—ROS response was investigated freshly isolated rat islets....
In experimental collagenase-induced osteoarthritis (OA) in the mouse, synovial lining macrophages are crucial mediating joint destruction. It was recently shown that adipose-derived stem cells (ASCs) express immunosuppressive characteristics. This study undertaken to explore effect of intraarticular injection ASCs on thickness and its relation pathology mouse OA.ASCs were isolated from fat surrounding inguinal lymph nodes cultured for 2 weeks. Experimental OA induced by collagenase into knee...
Objective— We hypothesized that adipose tissue may contain progenitors cells with cutaneous and angiogenic potential. Methods Results— Adipose tissue-derived stroma (ADSCs) were administrated to skin punched wounds of both nonirradiated irradiated mice (20 Gy, locally). At day14, ADSCs promoted dermal wound healing enhanced closure, viscolesticity, collagen secretion in mice. Interestingly, GFP-positive incorporated epidermal vivo expressed markers K5 K14. Cultured keratinocyte medium have...
The presence of brown adipose tissue (BAT) in human adults opens attractive perspectives to treat metabolic disorders. Indeed, BAT dissipates energy as heat via uncoupling protein (UCP)1. Brown adipocytes are located specific deposits or can emerge among white fat through the so-called browning process. Although numerous inducers have been shown drive this process, no study has investigated whether it could be controlled by metabolites. Here, we show that lactate, an important intermediate,...
Beside having roles in energy homeostasis and endocrine modulation, adipose tissue (AT) is now considered a promising source of mesenchymal stromal cells (adipose-derived or ASCs) for regenerative medicine. Despite numerous studies on cultured ASCs, native human ASCs are rarely investigated. Indeed, the phenotype their state, localization within AT comparison with bone marrow-derived (BM-MSCs) has been poorly To address these issues, stroma vascular fraction (SVF) was extracted cell subtypes...
In adults, adipose tissue is abundant and can be easily sampled using liposuction. Largely involved in obesity associated metabolic disorders, it now described as a reservoir of immature stromal cells. These cells, called adipose-derived cells (ADSCs) must distinguished from the crude vascular fraction (SVF) obtained after digestion tissue. ADSCs share many features with mesenchymal stem derived bone marrow, including paracrine activity, but they also display some specific features, greater...
Abstract Fibro-adipogenic progenitors (FAPs) play a crucial role in skeletal muscle regeneration, as they generate favorable niche that allows satellite cells to perform efficient regeneration. After injury, FAP content increases rapidly within the injured muscle, origin of which has been attributed their proliferation itself. However, recent single-cell RNAseq approaches have revealed phenotype and functional heterogeneity FAPs, raising question how this differentiation regenerative...
Several lines of evidence have supported a link betweeen adipose tissue and immunocompetent cells. This is illustrated in obesity, where excess adiposity impaired immune function been described both humans genetically obese rodents. In addition, numerous factors involved inflammatory response are secreted by preadipocytes macrophages. Here we show that proliferating cell primary cultures, develop phagocytic activity toward microorganisms. demonstrated phagocytosis assays confocal microscopy....
To investigate the physiological diversity in regulation and control of mitochondrial oxidative phosphorylation, we determined composition functional features respiratory chain muscle, heart, liver, kidney, brain. First, observed important variations content infrastructure via electron micrographs different tissue sections. Analyses enzyme by Western blot also showed large differences between tissues, good correlation with expression level transcription factor A activity citrate synthase. On...
Melatonin is involved in the regulation of seasonal obesity various species, including some rodents. This involvement has been demonstrated nonphotoperiodic rodents like rats, but only models enhanced body weight such as genetically obese or middle-aged rats. The aim this investigation was to determine effects melatonin on and metabolic parameters a model closer that observed Western populations, i.e. Sprague Dawley rats fed high-fat diet. They were treated for 3 wk with (30 mg/kg) 4 h after...
Abstract In contrast to the earlier contention, adult humans have been shown recently possess active brown adipose tissue with a potential of being metabolic significance. Up now, fat precursor cells not available for human studies. We previously that multipotent adipose-derived stem (hMADS) exhibit normal karyotype and high self-renewal ability; they are known differentiate into key properties white adipocytes, is, uncoupling protein two expression, insulin-stimulated glucose uptake,...