A5045163678- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Fatty Acid Research and Health
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Growth Hormone and Insulin-like Growth Factors
- Lipoproteins and Cardiovascular Health
- Muscle metabolism and nutrition
- Cancer, Hypoxia, and Metabolism
- Lipid metabolism and disorders
- Cardiovascular Disease and Adiposity
- Birth, Development, and Health
- Pancreatic function and diabetes
- Hormonal Regulation and Hypertension
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Diet, Metabolism, and Disease
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Cholesterol and Lipid Metabolism
- Health, Medicine and Society
Centre de Biologie du Développement
2002-2023
Centre National de la Recherche Scientifique
2009-2020
Université Côte d'Azur
2009-2020
Inserm
1992-2020
Institut de Biologie Valrose
2003-2018
GTx (United States)
2008
Laboratoire de Biologie du Développement
1986-2007
Centre Antoine Lacassagne
2003-2007
Centre de Recherche des Cordeliers
2007
Centre National pour la Recherche Scientifique et Technique (CNRST)
1989-2005
The ability of mammals to resist body fat accumulation is linked their expand the number and activity "brown adipocytes" within white depots. Activation β-adrenergic receptors (β-ARs) can induce a functional "brown-like" adipocyte phenotype. As cardiac natriuretic peptides (NPs) β-AR agonists are similarly potent at stimulating lipolysis in human adipocytes, we investigated whether NPs could mouse adipocytes acquire brown features, including capacity for thermogenic energy expenditure...
Stromal-vascular cells obtained from adult human subcutaneous adipose tissue were cultured in a chemically defined serum-free medium. In the presence of 0.2 nM triiodothyronine and 0.5 microM insulin, up to 25% able undergo terminal differentiation within 18 d, as assessed by lipid accumulation expression lipoprotein lipase (LPL) glycerol-3-phosphate dehydrogenase (GPDH) activities. Addition cortisol resulted potent dose-dependent stimulation process. Cortisol could be replaced dexamethasone...
White adipose tissue and liver are important angiotensinogen (AGT) production sites. Until now, plasma AGT was considered to be a reflection of hepatic production. Because concentration has been reported correlate with blood pressure, associated body mass index, we investigated whether is released locally into the stream. For this purpose, have generated transgenic mice either in which overexpressed or expression restricted tissue. This achieved by use aP2 adipocyte-specific promoter driving...
The gene product of the recently cloned mouse obese (ob) is important in regulating adipose tissue mass. ob RNA expressed specifically by adipocytes vivo each several different fat cell depots, including brown fat. also cultured 3T3-442A preadipocyte cells that have been induced to differentiate. Mice with lesions hypothalamus, as well mice mutant at db locus, express a 20-fold higher level tissue. These data suggest both and hypothalamus are downstream pathway regulates mass consistent...
ABSTRACT Embryonic stem cells, derived from the inner cell mass of murine blastocysts, can be maintained in a totipotent state vitro. In appropriate conditions embryonic cells have been shown to differentiate vitro into various derivatives all three primary germ layers. We describe this paper induce differentiation reliably and at high efficiency adipocytes. A prerequisite is treat early developing cell-derived embryoid bodies with retinoic acid for precise period time. Retinoic could not...
Here, we report the isolation of a human multipotent adipose-derived stem (hMADS) cell population from adipose tissue young donors. hMADS cells display normal karyotype; have active telomerase; proliferate >200 doublings; and differentiate into adipocytes, osteoblasts, myoblasts. Flow cytometry analysis indicates that are CD44+, CD49b+, CD105+, CD90+, CD13+, Stro-1−, CD34−, CD15−, CD117−, Flk-1−, gly-A−, CD133−, HLA-DR−, HLA-Ilow. Transplantation mdx mouse, an animal model Duchenne...
Exposure of preadipocytes to long chain fatty acids induces expression several gene markers adipocyte differentiation. This report describes the cloning, from a preadipocyte library, cDNA encoding acid-activated receptor, FAAR. The had characteristics and ligand-binding domains nuclear hormone receptors encoded 440 amino acid protein related peroxisome proliferator-activated receptors, PPAR. deduced sequence was 88% homologous that hNUC I, isolated human osteosarcoma cells. FAAR mRNA...
High fat intake is associated with mass gain through fatty acid activation of peroxisome proliferator-activated receptors δ and γ, which promote adipogenesis. We show herein that, compared to a combination specific agonists both or saturated, monounsaturated, ω-3 polyunsaturated acids, arachidonic (C20:4, ω-6) promoted substantially the differentiation clonal preadipocytes. This effect was blocked by cyclooxygenase inhibitors mimicked carbacyclin, suggesting role for prostacyclin receptor...
The product of the recently cloned mouse obese (ob) gene is likely to play an important role in a loop regulating size adipose tissue mass. hormonal regulation ob could affect adiposity. To investigate this point, effect insulin on expression was examined cells 3T3-F442A preadipocyte clonal line. mRNA absent from exponentially growing, undifferentiated as well confluent preadipose cells. Terminal differentiation leads detected by sensitive and quantitative ribonuclease protection assay. In...
The regulation of the expression adipose-related genes, i.e., aP2, adipsin, and glycerophosphate dehydrogenase (GPDH) by growth hormone (GH) polyamines, as well role fatty acids, have been investigated in polyaminedependent Ob1754 cells Ob1771 preadipose cells.Growth acts an obligatory for adipsin GPDH gene but its presence is not required aP2 gene.In fully differentiated cells, impairment acid synthesis glucose deprivation leads to inhibition expression, whereas genes remains...
Abstract In contrast to the earlier contention, adult humans have been shown recently possess active brown adipose tissue with a potential of being metabolic significance. Up now, fat precursor cells not available for human studies. We previously that multipotent adipose-derived stem (hMADS) exhibit normal karyotype and high self-renewal ability; they are known differentiate into key properties white adipocytes, is, uncoupling protein two expression, insulin-stimulated glucose uptake,...
Adipose tissue-derived stem cells offer tremendous potential for regenerative medicine. However, characterization of their self-renewal ability has not been performed yet, although it is a crucial feature in vitro expansion undifferentiated and vivo maintenance cell pools. We have undertaken the identification molecular events that are involved human multipotent adipose-derived (hMADS) from young donors, by assessing proliferation rate, to grow at single-cell level (clonogenicity),...
A clonal cell line that responds to insulin and lipolytic hormones has been established from the epididymal fat pad of C57BL/6J ob/ob mouse. This line, designated ob 17, a doubling time 12.5 or 19 hr in 10% 1% fetal calf serum, respectively. It presents heterogeneous chromosome number with 40% cells containing 35-44 chromosomes expresses characteristic H2-LA antigen. After cessation growth, 17 accumulate droplets triglycerides; this accumulation occurs significant extent even absence...
Cholesterol efflux was studied in cultured mouse adipose cells after preloading with low density lipoprotein cholesterol. Exposure to complexes containing human apolipoprotein A-IV and L-alpha-dimyristoylphosphatidylcholine (DMPC) as well particles but not A-I apolipoproteins showed that both artificial native A-IV-containing were able promote cholesterol at 37 degrees C a function of time concentration. The half-maximal concentration found be 0.3 X 10(-6) M for A-IV.DMPC complexes. Binding...
Lipolysis is the catabolic pathway by which triglycerides are hydrolyzed into fatty acids. Adipose triglyceride lipase (ATGL) and hormone-sensitive (HSL) have capacity to hydrolyze in vitro first ester bond of triglycerides, but their respective contributions whole cell lipolysis human adipocytes unclear. Here, we investigated roles HSL, ATGL, its coactivator CGI-58 basal forskolin-stimulated a white adipocyte model, hMADS cells. The express various components acid metabolism show lipolytic...
Prostacyclin (PGI2), the major metabolite of arachidonic acid in adipose tissue, has been shown to play a key role process preadipose cell differentiation vitro. Moreover, angiotensin-II (Ang II) is able induce production PGI2 suspensions isolated adipocytes as well interstitial fluid rat tissue. A possible Ang II control autocrine-paracrine adipogenic effect investigated, using cells Ob1771 preadipocyte clonal line cultured serum-free chemically defined medium. Whereas both and were produce...
Osteoporosis constitutes a major worldwide public health burden characterized by enhanced skeletal fragility. Bone metabolism is the combination of bone resorption osteoclasts and formation osteoblasts. Whereas increase in considered as main contributor loss that may lead to osteoporosis, this accompanied increased marrow adiposity. Osteoblasts adipocytes share same precursor cell an inverse relationship exists between two lineages. Therefore, identifying signaling pathways stimulate...
Fatty acids are important metabolic substrates for adipose tissue and act, in preadipose cells, as potent inducers of various proteins directly involved their metabolism. We have investigated the long-term effects fatty on conversion process Ob1771 cells to cells. Chronic exposure palmitate led, a dose-dependent manner, strong stimulation cell differentiation; this effect was confined terminal events whereas did not affect expression early genes related commitment adipoblasts Adipogenic...