A5101628771- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Cholesterol and Lipid Metabolism
- Regulation of Appetite and Obesity
- Cellular transport and secretion
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid Membrane Structure and Behavior
- RNA modifications and cancer
- Hepatitis B Virus Studies
- Drug Transport and Resistance Mechanisms
- Hepatitis C virus research
- Cervical Cancer and HPV Research
- Muscle activation and electromyography studies
- Erythrocyte Function and Pathophysiology
- Bacteriophages and microbial interactions
- Cellular Mechanics and Interactions
- Genetic Mapping and Diversity in Plants and Animals
- Biochemical Analysis and Sensing Techniques
- Transcranial Magnetic Stimulation Studies
- Stroke Rehabilitation and Recovery
- Chemotherapy-related skin toxicity
- Ubiquitin and proteasome pathways
- Cancer Treatment and Pharmacology
- RNA Research and Splicing
University of Florida
2000-2023
Florida College
2023
Fuzhou General Hospital of Nanjing Military Command
2023
Fujian Medical University
2022
Rockefeller University
1995-2019
Genomics (United Kingdom)
2018
Howard Hughes Medical Institute
1995-2018
Shanghai Jiao Tong University
2011-2018
BGI Group (China)
2012-2018
Laboratory of Molecular Genetics
2011-2016
Completion of genome sequences for the diploid Setaria italica reveals features C4 photosynthesis that could enable improvement polyploid biofuel crop switchgrass (Panicum virgatum). The genetic basis biotechnologically relevant traits, including drought tolerance, photosynthetic efficiency and flowering control, is also highlighted. Foxtail millet (Setaria italica), a member Poaceae grass family, an important food fodder in arid regions has potential use as biofuel. It model system other...
The gene product of the recently cloned mouse obese (ob) is important in regulating adipose tissue mass. ob RNA expressed specifically by adipocytes vivo each several different fat cell depots, including brown fat. also cultured 3T3-442A preadipocyte cells that have been induced to differentiate. Mice with lesions hypothalamus, as well mice mutant at db locus, express a 20-fold higher level tissue. These data suggest both and hypothalamus are downstream pathway regulates mass consistent...
Fsp27, a member of the Cide family proteins, was shown to localize lipid droplet and promote storage in adipocytes. We aimed understand biological role Fsp27 regulating adipose tissue differentiation, insulin sensitivity energy balance. Fsp27−/− mice Fsp27/lep double deficient were generated we examined adiposity, whole body metabolism, BAT WAT morphology, sensitivity, mitochondrial activity, gene expression changes these mouse strains. Furthermore, isolated embryonic fibroblasts (MEFs) from...
The recently identified mouse obese (ob) gene apparently encodes a secreted protein that may function in the signaling pathway of adipose tissue. Mutations ob are associated with early development gross obesity. A detailed knowledge concerning RNA expression pattern and precise genomic location human homolog, OB gene, would facilitate examination role this inheritance Northern blot analysis revealed is present at high level tissue but much lower levels placenta heart. undetectable wide range...
Infection by chronic hepatitis B virus (HBV) is one of the major causes liver cirrhosis and primary hepatocellular carcinoma (HCC). Viral DNA integration into host cell genome a key mechanism hepatocarcinogenesis. However, molecular characterization potential clinical implications HBV patients suffering from different HCC remain unclear. In this study, we analyzed integrations in with using probe-based capturing next-generation sequencing. The results revealed that sizes ranged 28 bp to 3215...
Abstract Background The objective of the study was to investigate effect different types bile acids on proliferation cholangiocarcinoma and potential molecular mechanisms. Methods PCR assay Western blot were performed detect expression farnesoid × receptor (FXR) in mRNA protein level. Immunohistochemical analysis carried out monitor FXR tissues from 26 patients 10 normal controls. effects vivo tumor growth also studied nude mouse model. Results Free induced an increased FXR; contrary,...
Background To better understand the pathogenesis of cervical cancer (CC), we systematically analysed genomic variation and human papillomavirus (HPV) integration profiles intraepithelial neoplasia (CIN) CC. Methods We performed whole-genome sequencing or whole-exome 102 tumour-normal pairs probe capture 45 CCs, 44 CIN samples 25 normal samples, constructed strict integrated workflow analysis. Results Mutational analysis identified eight significantly mutated genes in CC including four ( FAT1...
Transcranial magnetic stimulation and peripheral repetitive (rPMS), as non-invasive neuromodulation techniques, can promote functional recovery in patients with post-stroke spasticity (PSS), but the effects of transcranial combined on PSS remain largely unknown. Therefore, we examined low-frequency (LF-rTMS) rPMS its potential neural correlates to behavioral improvements.Forty-nine were divided randomly into three groups: a group (n = 20), LF-rTMS 15), control 14). The received treatment,...
Abstract Background Cide family proteins including Cidea, Cideb and Cidec/Fsp27, contain an N-terminal CIDE-N domain that shares sequence similarity to the CAD (NCD) of DNA fragmentation factors Dffa/Dff45/ICAD Dffb/Dff40/CAD, a unique C-terminal CIDE-C domain. We have previously shown are newly emerged regulators closely associated with development metabolic diseases such as obesity, diabetes liver steatosis. They modulate many processes lipolysis, thermogenesis TAG storage in brown adipose...
We observed that free bile acids (CA, DCA, and CDCA) inhibited the growth of cholangiocarcinoma cells by promoting cell apoptosis, while conjugated (GCA, GDCA, GCDCA) stimulated growth. Consistently, we found GDCA tumor CDCA decreased in xenografted mice. Further, phosphorylated IkB was downregulated acids, upregulated acids. IL-6 COX-2 were increased Collectively, these results suggest regulate modulating NF-kB pathway.
Significance Leptin gene expression is highly correlated with the lipid content of individual fat cells, suggesting that it regulated by a “fat-sensing” signal transduction pathway. This possibility thus analogous to identification cholesterol-sensing pathway studying regulation LDL receptor intracellular cholesterol. Several lines investigation have suggested that, in addition adipocytes, liver, neurons, and other cell types can sense changes content, although molecular mechanisms are...
Scap is a polytopic protein of the endoplasmic reticulum (ER) that controls cholesterol homeostasis by transporting sterol regulatory element-binding proteins (SREBPs) from ER to Golgi complex. has eight transmembrane helices (TM) joined four small hydrophilic loops and three large loops. Two (Loops 1 7) are in lumen, other loop (Loop 6) faces cytosol where it binds COPII initiate transport Golgi. Cholesterol binding Loop alters configuration 6, precluding preventing exit ER. Here, we create...
Brown and white adipose tissues (BAT WAT) play critical roles in controlling energy homeostasis the development of obesity diabetes. The mouse Fat-Specific protein 27 (FSP27), a member cell death-inducing DFF45-like effector (CIDE) family, is expressed both BAT WAT associated with lipid droplets. Over-expression FSP27 promotes storage, whereas deficient mice have improved insulin sensitivity are resistant to diet-induced obesity. In addition, FSP27-deficient adipocytes reduced smaller...
Cholesterol homeostasis is mediated by Scap, a polytopic endoplasmic reticulum (ER) protein that transports sterol regulatory element-binding proteins from the ER to Golgi, where they are processed forms activate cholesterol synthesis. Scap has eight transmembrane helices and two large luminal loops, designated Loop1 Loop7. We earlier provided indirect evidence binds Loop7, allowing bind COPII for transport in coated vesicles. When rises, it Loop1. hypothesized this causes dissociation...
Abstract The underlying cellular mechanism of anabolic effect recovered by inserting rest is not fully understood. In this work, we studied the role F-actin regulated mechanosensitive channel(s) re-activation in mechanosensitivity modulation vitro. Results showed that steady fluid shear stress (sFSS) stimulation with 30-min period was more potential increasing alkalinephosphatase (ALP) activity than 10 and 0-min periods, insertion 30 min, but 0 or [Ca2+]i transient contribution channel(s)....
Resistance training has been known to have a positive effect on muscle performance in exercisers. Whole-body electromyostimulation (WB-EMS) is advertised as smooth, time-efficient, and highly individualized resistance technology. The purpose of this study evaluate the effects WB-EMS maximum isometric elbow strength body composition moderately trained males comparison traditional training. was randomized controlled single-blind trial. Twenty, trained, male participants (25.15 ± 3.84, years)...
To better understand the pathogenesis of cervical cancer (CC), we systematically analyzed genomic variation and human papillomavirus (HPV) integration profiles intraepithelial neoplasia (CIN) CC. We performed whole-genome sequencing (WGS) or whole-exome (WES) 102 tumor-normal pairs probe capture (HPCS) 45 CCs, 44 samples, 25 normal constructed strict integrated workflow analysis. Mutational analysis identified eight significantly mutated genes in CC including four (FAT1, MLL3, MLL2, FADD),...
Abstract Leptin expression decreases after fat loss and is increased when obesity develops its proper quantitative regulation essential for the homeostatic control of mass. We previously reported that a distant leptin enhancer (LE1), 16kb upstream from transcription start site (TSS), confers fat-specific in BAC transgenic reporter mouse (BACTG). However this other elements we identified do not account changes accompany alterations adipose In report, used ATAC-seq to identify 17bp...