A5025422510- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Lung Cancer Treatments and Mutations
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Colorectal Cancer Treatments and Studies
- RNA Interference and Gene Delivery
- Lipid metabolism and biosynthesis
- Heat shock proteins research
- Mycobacterium research and diagnosis
- Cervical Cancer and HPV Research
- interferon and immune responses
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- PARP inhibition in cancer therapy
- RNA and protein synthesis mechanisms
- Hepatitis B Virus Studies
- Radiomics and Machine Learning in Medical Imaging
- FOXO transcription factor regulation
- MicroRNA in disease regulation
National Cancer Centre Singapore
2016-2025
Institute of Medical Biology
2015
Agency for Science, Technology and Research
2013-2015
Institute of Molecular and Cell Biology
1998-2010
Tsinghua University
2008
Hong Kong University of Science and Technology
2008
University of Hong Kong
2008
Fsp27, a member of the Cide family proteins, was shown to localize lipid droplet and promote storage in adipocytes. We aimed understand biological role Fsp27 regulating adipose tissue differentiation, insulin sensitivity energy balance. Fsp27−/− mice Fsp27/lep double deficient were generated we examined adiposity, whole body metabolism, BAT WAT morphology, sensitivity, mitochondrial activity, gene expression changes these mouse strains. Furthermore, isolated embryonic fibroblasts (MEFs) from...
Abstract The human genome contains more than 200,000 gene isoforms. However, different isoforms can be highly similar, and with an average length of 1.5kb remain difficult to study short read sequencing. To systematically evaluate the ability transcriptome at a resolution individual we profiled 5 cell lines cDNA sequencing Nanopore long direct RNA, amplification-free cDNA, PCR-cDNA protocols showed high level consistency, RNA being most similar. While reads generated comparable expression...
Abstract The human genome contains instructions to transcribe more than 200,000 RNAs. However, many RNA transcripts are generated from the same gene, resulting in alternative isoforms that highly similar and remain difficult quantify. To evaluate ability study transcript expression, we profiled seven cell lines with five different RNA-sequencing protocols, including short-read cDNA, Nanopore long-read direct RNA, amplification-free cDNA PCR-amplified sequencing, PacBio IsoSeq, multiple...
Genomics-driven cancer therapeutics has gained prominence in personalized treatment. However, its utility indications lacking biomarker-driven treatment strategies remains limited. Here we present a "phenotype-driven precision-oncology" approach, based on the notion that biological response to perturbations, chemical or genetic, ex vivo patient-individualized models can serve as predictive biomarkers for therapeutic clinic. We generated library of "screenable" patient-derived primary...
The fat-specific protein 27 (Fsp27), a localized to lipid droplets (LDs), plays an important role in controlling storage and mitochondrial activity adipocytes. Fsp27-null mice display increased energy expenditure are resistant high fat diet-induced obesity diabetes. However, little is known about how the Fsp27 regulated. Here, we show that stability controlled by ubiquitin-dependent proteasomal degradation pathway ubiquitination of regulated three lysine residues located C-terminal region....
Cell death-inducingDFF45-like effector (CIDE)-B is a member of the novel family apoptosis-inducing factors that share homology with N-terminal region DFF, DNA fragmentation factor. The molecular mechanism CIDE-B-induced apoptosis unclear. We have shown here CIDE-B protein localized in mitochondria and forms homodimers heterodimers other members. Serial deletion analyses suggest localization signal dimerization interface are overlapped to 30 amino acid residues at C-terminal CIDE-B....
// Yuezhen Xue 1, 5 , Shen Yon Toh 1 Pingping He Thimothy Lim 2 Diana 3 Chai Ling Pang 4 Jean-Pierre Abastado Françoise Thierry Institute of Medical Biology, A*STAR, Singapore Department Gynaecological Oncology, KK Women's and Children's Hospital, Pathology, National University Immunology Network, Current address: p53 Laboratory, Correspondence to: Xue, e-mail: yzxue@p53lab.a-star.edu.sg Keywords: HPV16-E2, DNA damage response, cell cycle, prophase, cervical intraepithelial neoplasia...
Abstract While EGFR tyrosine kinase inhibitors (TKIs) can elicit significant tumor shrinkage in the clinic, drug resistance inevitably ensues, likely due to a persistent subpopulation. T790M mutation is common mechanism found 60% of tumors progressing on TKI. The aim this study uncover novel mechanisms gefitnib-resistant (GR) NSCLC by selecting TKI naïve PC9 single cell clones (SCC) differing stemness and vulnerabilities gefitinib (G), inducing through sublethal exposures. A high throughput...
Abstract Squamous-cell cancers (SCCs) represent one of the commonest lethal malignancy worldwide. Despite evidence demonstrating that majority are addicted to Epidermal Growth Factor Receptor (EGFR)-signaling, targeting with monoclonal antibodies or tyrosine-kinase inhibitors (TKIs) has been met limited success. We recently demonstrated TKI-sensitivity is modulated by a long non-coding RNA (lncRNA) situated in antisense strand EGFR (EGFR-AS1). ‘Synonymous' alterations EGFR-AS1 affected...