A5057164629- Antifungal resistance and susceptibility
- Cancer-related Molecular Pathways
- Fungal Infections and Studies
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- ATP Synthase and ATPases Research
- Cancer therapeutics and mechanisms
- Microtubule and mitosis dynamics
- Immunotherapy and Immune Responses
- Lipid metabolism and biosynthesis
- Cancer, Hypoxia, and Metabolism
- Protein Tyrosine Phosphatases
- Bacterial Genetics and Biotechnology
- Adipose Tissue and Metabolism
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Endoplasmic Reticulum Stress and Disease
- Sphingolipid Metabolism and Signaling
- Toxoplasma gondii Research Studies
- Glycosylation and Glycoproteins Research
- Mechanisms of cancer metastasis
Beijing Normal University
2010-2019
Fu Wai Hospital
2009
Chinese Academy of Medical Sciences & Peking Union Medical College
2009
Beth Israel Deaconess Medical Center
1957
New York Medical College
1956
Ubiquitin C-terminal hydrolase-L1 (UCHL1) belongs to the family of deubiquitinating enzymes (DUBs), which is involved in ubiquitin-dependent proteolytic system. Previously, we have reported that upregulation UCHL1 related lymph node metastasis colorectal cancer (CRC). However, its molecular mechanisms remain elusive. In this study, transfected pcDNA3.1/UCHL1 and pcDNA3.1/UCHL1-C90S mutant into HCT8 cells. The changes biological features these stable transfectants were examined both vitro...
The fat-specific protein 27 (Fsp27), a localized to lipid droplets (LDs), plays an important role in controlling storage and mitochondrial activity adipocytes. Fsp27-null mice display increased energy expenditure are resistant high fat diet-induced obesity diabetes. However, little is known about how the Fsp27 regulated. Here, we show that stability controlled by ubiquitin-dependent proteasomal degradation pathway ubiquitination of regulated three lysine residues located C-terminal region....
Ovarian cancer G protein coupled receptor 1 (OGR1) mediates inhibitory effects on cell migration in human prostate and ovarian cells. However, the mechanisms signaling pathways that mediate these are essentially unknown.
Lysophosphatidic acid (LPA), as an endogenous lipid mediator, has been revealed to regulate many important biological and pathophysiological processes via specific G-protein-coupled receptors termed LPA1-5. We have previously shown that LPA antagonized the apoptosis of mesenchymal stem cells (MSCs) induced by hypoxia serum deprivation (hypoxia/SD), mimicking ischemic myocardium microenvironment. Whether same potentially beneficial effect on MSCs in vivo is unknown. Here we demonstrated...
Summary β‐1,6‐glucan is an important component of the fungal cell wall. The synthase gene KRE6 was thought to be essential in pathogen Candida albicans because it could not deleted previous efforts. Also, role its homolog SKN1 unclear deletion caused no defects. Here, we report construction and characterization kre6 Δ/Δ, skn1 Δ/Δ mutants C. . While deleting or had obvious phenotypic consequence, both slow growth, separation failure, wall abnormalities, diminished hyphal poor biofilm...
Brown and white adipose tissues (BAT WAT) play critical roles in controlling energy homeostasis the development of obesity diabetes. The mouse Fat-Specific protein 27 (FSP27), a member cell death-inducing DFF45-like effector (CIDE) family, is expressed both BAT WAT associated with lipid droplets. Over-expression FSP27 promotes storage, whereas deficient mice have improved insulin sensitivity are resistant to diet-induced obesity. In addition, FSP27-deficient adipocytes reduced smaller...
The pathogenic fungus Candida albicans switches from yeast growth to filamentous in response genotoxic stresses, which phosphoregulation of the checkpoint kinase Rad53 plays a crucial role. Here we report that Pph3/Psy2 phosphatase complex, known be involved dephosphorylation, is required for cellular responses DNA-damaging agent methyl methanesulfonate (MMS) but not DNA replication inhibitor hydroxyurea (HU) C. albicans. Deletion either PPH3 or PSY2 resulted enhanced during MMS treatment...
Therapeutic cloning, whereby somatic cell nuclear transfer is used to generate customized embryonic stem cells (NT-ES) from differentiated of specific individuals, has been successfully performed in mice and non-human primates. Safety concerns have prevented this technology being potentially applied humans, as severely abnormal phenotypes observed cloned animals. Although it demonstrated that the transcriptional profiles developmental potentials ES derived blastocysts are identical those...
Summary β‐1,6‐glucan is an important cell wall component of Candida albicans. Deleted mutants the two synthase genes KRE6 and SKN1 are viable albeit with a range defects including slow growth. It remains unclear whether synthesis not required under culture conditions or compensatory mechanisms exist in C. albicans . Here, we report that depleting synthases leads to significant increase chitin levels through posttranscriptional regulation Chs3 which maintains viability. And chs3 Δ/Δ cells...
Septins are a component of the cytoskeleton and play important roles in diverse cellular processes including cell cycle control, cytokinesis polarized growth. In fungi, septin organization, dynamics function regulated by phosphorylation, several kinases responsible for phosphorylation septins have been identified. However, little is known about phosphatases that dephosphorylate septins. Here, we report characterization Tpd3, structural subunit PP2A family phosphatases, pathogenic fungus...
PP2A-like phosphatases share high homology with PP2A enzymes and are composed of a catalytic subunit regulatory subunit. In Candida albicans, the SIT4 regulates cell growth, morphogenesis, virulence. However, functions its subunits remain unclear. Here, by analysis co-IP experiments, we identified two in C. SAP155 (orf19.642) SAP190 (orf19.5160). We constructed sit4Δ/Δ, sap155Δ/Δ, sap190Δ/Δ, sap155Δ/Δ sap190Δ/Δ mutants found that deleting had no apparent phenotypic consequence, while caused...
Successful pathogens must be able to swiftly respond and repair DNA damages inflicted by the host defence. The replication protein A (RPA) complex plays multiple roles in damage response is regulated phosphorylation. However, regulators of RPA phosphorylation remain unclear. Here, we investigated Rfa2 pathogenic fungus Candida albicans. Rfa2, a RFA subunit, phosphorylated when inhibited hydroxyurea dephosphorylated during recovery. By screening phosphatase mutant library, found that Pph3...
Melanoma-associated antigen A1 (MAGEA1) and BORIS are members of the cancer testis antigens (CTA) family. Their functions expression regulation mechanisms not fully understood. In this study, we revealed new regulatory MAGEA1 in breast cells, which were investigated parental genetically manipulated cells via gene overexpression or siRNA interference-mediated down-regulation. We identified interaction between CTCF, was required for binding to promoter critical recruitment DNMT3a. A protein...
Rfa2 is a ssDNA (single-stranded DNA)-binding protein that plays an important role in DNA replication, recombination and repair. regulated by phosphorylation, which alters its protein-protein interaction protein-DNA interaction. In the present study, we found Pph3-Psy2 phosphatase complex responsible for dephosphorylation both during normal G1-phase under replication stress Candida albicans. Phosphorylated extracted from pph3Δ or psy2Δ G1 cells exhibited diminished binding affinity to dsDNA...
Genotoxic stress causes DNA damage or stalled replication and filamentous growth in the pathogenic fungus Candida albicans The checkpoint kinase Rad53 critically regulates by phosphorylation effectors that execute response. itself is activated inactivated dephosphorylation. Previous studies have suggested phosphatase Pph3 dephosphorylates Rad53. Here, we used mass spectrometry mutagenesis to identify dephosphorylation sites on C. We found serine residues 351, 461 477, which were...