A5100612658- Sphingolipid Metabolism and Signaling
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related molecular mechanisms research
- Lipid Membrane Structure and Behavior
- Cancer, Hypoxia, and Metabolism
- Ovarian cancer diagnosis and treatment
- Epigenetics and DNA Methylation
- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- Receptor Mechanisms and Signaling
- Cancer, Lipids, and Metabolism
- FOXO transcription factor regulation
- Hippo pathway signaling and YAP/TAZ
- RNA modifications and cancer
- Caveolin-1 and cellular processes
- Estrogen and related hormone effects
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Erythrocyte Function and Pathophysiology
- Thyroid Cancer Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Metabolomics and Mass Spectrometry Studies
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
First Affiliated Hospital of Zhengzhou University
2025
Daping Hospital
2011-2024
Army Medical University
2011-2024
Gansu University of Traditional Chinese Medicine
2024
Sun Yat-sen University
2024
Third Affiliated Hospital of Sun Yat-sen University
2024
Indiana University – Purdue University Indianapolis
2013-2023
Indiana University School of Medicine
2013-2023
Xuzhou Medical College
2023
Highlights•Design of a novel high-throughput assay for cellular mechanosensation•An RNAi screen identifies GPR68 as mechanosensor•GPR68 is necessary and sufficient responses to fluid shear stress•GPR68 required flow-induced dilation remodeling in miceSummaryMechanotransduction plays crucial role vascular biology. One example this the local regulation resistance via flow-mediated (FMD). Impairment process hallmark endothelial dysfunction precursor wide array diseases, such hypertension...
Abstract Zinc is an essential micronutrient that plays a role in the structural or enzymatic functions of many cellular proteins. Cellular zinc homeostasis involves opposing action two families metal transporters: ZnT (SLC30) family to reduce cytoplasmic concentrations and ZIP (SLC39) increase concentrations. Fluctuations intracellular levels mediated by these transporter affect signaling pathways involved normal cell development, growth, differentiation death. Consequently, changes...
Abstract Objective: To determine whether lysophosphatidic acid (LPA) and other lysophospholipids (LPL) are useful markers for diagnosis and/or prognosis of ovarian cancer in a controlled setting. Method: Plasma samples were collected from patients healthy control women Hillsborough Pinellas counties, Florida, processed at the University South Florida H. Lee Moffitt Cancer Center Research Institute (Moffitt). Case with epithelial (n = 117) subjects 27) participated study. Blinded LPL...
Although the biological actions of cell membrane and serum lipid lysophosphatidylcholine (LPC) in atherosclerosis systemic autoimmune disease are well recognized, LPC has not been linked to a specific cell-surface receptor. We show that is high-affinity ligand for G2A, lymphocyte-expressed G protein–coupled receptor whose genetic ablation results development autoimmunity. Activation G2A by increased intracellular calcium concentration, induced internalization, activated ERK mitogen-activated...
Sphingosylphosphorylcholine (SPC) and lysophosphatidylcholine (LPC) are bioactive lipid molecules involved in numerous biological processes. We have recently identified ovarian cancer G protein-coupled receptor 1 (OGR1) as a specific high affinity for SPC, G2A with LPC, but low SPC. Among receptors, GPR4 shares highest sequence homology OGR1 (51%). In this work, we not only another also albeit of lower affinity. Both SPC LPC induce increases intracellular calcium concentration GPR4-,...
Plasma levels of lysophospholipids were evaluated as potential biomarkers for colorectal cancer (CRC), where a highly reliable and minimally invasive blood test is lacking.Patients with CRC (n = 133) control subjects 125) recruited through the Cleveland Clinic. Preoperative plasma samples analyzed lysophospholipid using liquid chromatography mass spectrometry in blinded fashion. Participants randomly divided 2:1 ratio into "training set" (TS) "validation (VS). Logistic regression models used...
Abstract Lysophosphatidic acid (LPA) is both a potential marker and therapeutic target for ovarian cancer. It critical to identify the sources of elevated LPA levels in ascites blood patients with We show here that human peritoneal mesothelial cells constitutively produce LPA, which accounts significant portion chemotactic activity conditioned medium from cancer cells. Both production by can be blocked HELSS [an inhibitor calcium-independent phospholipase A2 (iPLA2)] AACOCF3 cytosolic PLA2...
Rheumatoid arthritis is a destructive arthropathy characterized by chronic synovial inflammation that imposes substantial socioeconomic burden. Under the influence of proinflammatory milieu, fibroblasts (SFs), main effector cells in disease pathogenesis, become activated and hyperplastic, releasing factors tissue-remodeling enzymes. This study shows arthritic SFs from human patients animal models express significant quantities autotaxin (ATX; ENPP2), lysophospholipase D catalyzes conversion...
The Hippo-YAP signaling pathway is altered and implicated as oncogenic in many human cancers. However, extracellular signals that regulate the mammalian Hippo have remained elusive until very recently when it was shown regulated by G-protein-coupled receptor (GPCR) ligands including lysophosphatidic acid (LPA) sphingosine 1-phosphophate (S1P). LPA inhibits Lats kinase activity HEK293 cells, but potential involvement of a protein phosphatase not investigated. regulators YAP dephosphorylation...
Autotaxin (ATX) is an extracellular lysophospholipase D that generates lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC). Both ATX and LPA have been shown to be involved in many cancers. However, the functional role of regulation expression human hepatocellular carcinoma (HCC) remain elusive. In this study, was evaluated tissues 38 HCC 10 normal control subjects. detected mainly tumor cells within tissue sections its over-expression specifically correlated with inflammation...
Significance We found that Twist1 is coexpressed with multiple other epithelial–mesenchymal transition (EMT)-inducing transcription factors (TFs) in small subsets of primary and circulating breast tumor cells. Together, they program a partial EMT basal-like phenotype cells, which most likely are responsible for disseminating into the circulation metastasizing to lung mice. Targeting largely diminishes all cellular features phenotypes indicating may be target inhibiting cancer metastasis....
A bstract : We previously reported that lysophosphatidic acid (LPA) represents a potential biomarker for ovarian and other gynecologic cancers. To further improve the accuracy potentially increase sensitivity specificity of assay, we developed an electrospray ionization mass spectrometry (ESI‐MS)‐based method to analyze LPA related lysophospholipids. LPA, lysophosphatidylinositol (LPI), lysophosphatidylserine (LPS), lysophosphatidylcholine (LPC) could be detected with high (in low pmol...
Abstract Lysophosphatidic acid (LPA) is elevated in ascites of ovarian cancer patients and stimulates growth other activities cells vitro. Tissue hypoxia a critical factor for tumor aggressiveness metastasis cancers. We tested whether the hypoxic influences effects LPA on cells. found that ascitic fluids were vivo. Enhanced cellular responsiveness to LPA, including migration and/or invasion cells, was observed under conditions. This enhancement could be completely blocked by geldanamycin or...