A5066449841- Erythrocyte Function and Pathophysiology
- Ion channel regulation and function
- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Blood properties and coagulation
- Chemical and Physical Studies
- Plant and Biological Electrophysiology Studies
- Pain Mechanisms and Treatments
- Biochemical Analysis and Sensing Techniques
- Cellular Mechanics and Interactions
- Herbal Medicine Research Studies
- Connexins and lens biology
- Circadian rhythm and melatonin
- Phytochemicals and Antioxidant Activities
- Nanopore and Nanochannel Transport Studies
- Hemoglobin structure and function
- Ion Transport and Channel Regulation
- Postharvest Quality and Shelf Life Management
- Lipid Membrane Structure and Behavior
- Hemoglobinopathies and Related Disorders
- Olfactory and Sensory Function Studies
- Nerve injury and regeneration
- Neuroscience of respiration and sleep
- Thermoregulation and physiological responses
- Neurogenetic and Muscular Disorders Research
Howard Hughes Medical Institute
2016-2025
Scripps Institution of Oceanography
2019-2025
Scripps (United States)
2023-2025
Scripps Research Institute
2015-2024
Scripps Health
2020-2023
ORCID
2022
Memorial
2021
General Atomics (United States)
2020
MED Institute
2018
Novartis Foundation
2009-2015
Mechanical Responders Identified Although many cells appear to respond mechanical stimulation through increased conductance of ion channels in the plasma membrane, actual that mediate these effects—which are important diverse processes from hearing and touch control blood pressure—have remained elusive. Coste et al. (p. 55 , published online 2 September) used RNA interference decrease expression candidate genes systematically a mouse neuroblastoma cell line identified two encode proteins,...
High-throughput gene expression profiling has become an important tool for investigating transcriptional activity in a variety of biological samples. To date, the vast majority these experiments have focused on specific processes and perturbations. Here, we generated analyzed from set samples spanning broad range conditions. Specifically, profiled 91 human mouse across diverse array tissues, organs, cell lines. Because predominantly come normal physiological state mouse, this dataset...
Mechanical and thermal cues stimulate a specialized group of sensory neurons that terminate in the skin. Three members transient receptor potential (TRP) family channels are expressed subsets these activated at distinct physiological temperatures. Here, we describe cloning characterization novel thermosensitive TRP channel. TRPV3 has unique threshold: It is innocuous (warm) temperatures shows an increased response noxious specifically keratinocytes; hence, skin cells capable detecting heat...
Significance Ion channels that are activated by mechanical force have been implicated in numerous physiological systems. In mammals, the identity of these remains poorly understood. We recently described Piezos as evolutionarily conserved mechanically ion and showed Piezo2 is required for activation touch receptors skin. Here we show Piezo1 a critical component endothelial cell mechanotransduction embryonic development. expressed cells fluid shear stress. Loss affects ability to alter their...
Environmental temperature is thought to be directly sensed by neurons through their projections in the skin. A subset of mammalian transient receptor potential (TRP) family ion channels has been implicated this process. These "thermoTRPs" are activated at distinct thresholds and typically expressed sensory neurons. TRPV3 heat (>33 degrees C) and, unlike most thermoTRPs, mouse keratinocytes. We found that null mice have strong deficits responses innocuous noxious but not other modalities;...
Piezo ion channels are activated by various types of mechanical stimuli and function as biological pressure sensors in both vertebrates invertebrates. To date, the only means to activate whether other modes activation exist is not known. In this study, we screened ∼3.25 million compounds using a cell-based fluorescence assay identified synthetic small molecule termed Yoda1 that acts an agonist for human mouse Piezo1. Functional studies cells revealed affects sensitivity inactivation kinetics...
Red blood cells (RBCs) experience significant mechanical forces while recirculating, but the consequences of these are not fully understood. Recent work has shown that gain-of-function mutations in mechanically activated Piezo1 cation channels associated with dehydrating RBC disease xerocytosis, implicating a role mechanotransduction volume regulation. However, mechanisms by which result dehydration unknown. In this study, we show RBCs exhibit robust calcium entry response to stretch and is...