A5054637908- Cannabis and Cannabinoid Research
- Click Chemistry and Applications
- Peptidase Inhibition and Analysis
- Pancreatic function and diabetes
- Sleep and Wakefulness Research
- Chemical Synthesis and Analysis
- Diet, Metabolism, and Disease
- Advanced Proteomics Techniques and Applications
- Neurotransmitter Receptor Influence on Behavior
- Protein Degradation and Inhibitors
- Neuroscience and Neuropharmacology Research
- Ubiquitin and proteasome pathways
- Forensic Toxicology and Drug Analysis
- Alcohol Consumption and Health Effects
- Neuroscience of respiration and sleep
- Biotin and Related Studies
- Monoclonal and Polyclonal Antibodies Research
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Polyamine Metabolism and Applications
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Peroxisome Proliferator-Activated Receptors
- Histone Deacetylase Inhibitors Research
Scripps Research Institute
2016-2025
Scripps Institution of Oceanography
2008-2025
Scripps (United States)
2008-2025
Torrey Pines Institute For Molecular Studies
2016-2025
Vividion Therapeutics (United States)
2024
Stony Brook University
2021
Tufts University
2021
Aix-Marseille Université
2021
Centre National de la Recherche Scientifique
2021
ORCID
2020
The medicinal properties of marijuana have been recognized for centuries, but clinical and societal acceptance this drug abuse as a potential therapeutic agent remains fiercely debated. An attractive alternative to marijuana-based therapeutics would be target the molecular pathways that mediate effects drug. To date, these neural signaling shown comprise cannabinoid receptor (CB 1 ) binds active constituent marijuana, tetrahydrocannabinol (THC), postulated endogenous CB ligand anandamide....
With the postgenome era rapidly approaching, new strategies for functional analysis of proteins are needed. To date, proteomics efforts have primarily been confined to recording variations in protein level rather than activity. The ability profile classes on basis changes their activity would greatly accelerate both assignment function and identification potential pharmaceutical targets. Here, we describe chemical synthesis utility an active-site directed probe visualizing dynamics...
Toward the goal of assigning function to tens thousands protein products encoded by eukaryotic and prokaryotic genomes, field proteomics requires new technologies that can functionally characterize proteins within dynamic environment cell, where these biomolecules are subject myriad posttranslational modifications actions endogenous activators inhibitors. Here, we report an advanced strategy for activity-based profiling (ABPP) addresses this important need. We show several enzymes be labeled...
Phospholipase A(2)(PLA(2)) enzymes are considered the primary source of arachidonic acid for cyclooxygenase (COX)-mediated biosynthesis prostaglandins. Here, we show that a distinct pathway exists in brain, where monoacylglycerol lipase (MAGL) hydrolyzes endocannabinoid 2-arachidonoylglycerol to generate major arachidonate precursor pool neuroinflammatory MAGL-disrupted animals neuroprotection parkinsonian mouse model. These spared hemorrhaging caused by COX inhibitors gut, prostaglandins...
A molecule isolated from the cerebrospinal fluid of sleep-deprived cats has been chemically characterized and identified as cis -9,10-octadecenoamide. Other fatty acid primary amides in addition to -9,10-octadecenoamide were natural constituents cat, rat, human, indicating that these compounds compose a distinct family brain lipids. Synthetic induced physiological sleep when injected into rats. Together, results suggest may represent previously unrecognized class biological signaling molecules.
Cellular communication in the nervous system is mediated by chemical messengers that include amino acids, monoamines, peptide hormones, and lipids. An interesting question how neurons regulate signals are transmitted membrane-embedded Here, we report 2.8 angstrom crystal structure of integral membrane protein fatty acid amide hydrolase (FAAH), an enzyme degrades members endocannabinoid class signaling lipids terminates their activity. The FAAH complexed with arachidonyl inhibitor reveals a...
Acetaminophen (paracetamol) is a popular domestic analgesic and antipyretic agent with weak anti-inflammatory action low incidence of adverse effects as compared aspirin other non-steroidal drugs. Here we show that acetaminophen, following deacetylation to its primary amine, conjugated arachidonic acid in the brain spinal cord form potent TRPV1 agonist N-arachidonoylphenolamine (AM404). This conjugation absent mice lacking enzyme fatty amide hydrolase. AM404 also inhibits purified...
Metalloproteases (MPs) are a large and diverse class of enzymes implicated in numerous physiological pathological processes, including tissue remodeling, peptide hormone processing, cancer. MPs tightly regulated by multiple posttranslational mechanisms vivo , hindering their functional analysis conventional genomic proteomic methods. Here we describe general strategy for creating activity-based probes coupling zinc-chelating hydroxamate to benzophenone photocrosslinker, which promote...