A5039666775- Yersinia bacterium, plague, ectoparasites research
- Pharmacological Effects of Natural Compounds
- Antibiotic Resistance in Bacteria
- Immune Response and Inflammation
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Bacterial Genetics and Biotechnology
- Bartonella species infections research
- Streptococcal Infections and Treatments
- Infections and bacterial resistance
- Infective Endocarditis Diagnosis and Management
- Probiotics and Fermented Foods
- Congenital Heart Disease Studies
- Vector-borne infectious diseases
- Vibrio bacteria research studies
- Antibiotic Use and Resistance
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
- Venous Thromboembolism Diagnosis and Management
Heidelberg University
2020-2025
University Hospital Heidelberg
2020-2025
German Center for Infection Research
2013-2022
Institute of Medical Microbiology and Hygiene
2012-2022
Universitätsklinikum Tübingen
2007-2021
University of Tübingen
2011-2020
ARUP Institute for Clinical and Experimental Pathology
2019
University of Utah
2019
ARUP Laboratories (United States)
2019
University Children's Hospital Tübingen
2014-2017
Using fluorescent in situ hybridization (FISH) with rRNA-targeted fluorescently labelled oligonucleotide probes, pathogens were rapidly detected and identified positive blood culture bottles without cultivation biotyping. In this study, 115 cultures a growth index as determined by continuous-reading automated system examined both conventional laboratory methods FISH. For purpose, probes that allowed identification of approximately 95% those typically associated bacteremia produced. The...
IL-12 p40–related cytokines such as p35/p40 heterodimer and IL-23 (p19/p40) are potent regulators of adaptive immune responses. Little is known, however, about the transcriptional regulation p40 gene in vivo. In an attempt toward this goal, we have generated transgenic mice expressing firefly luciferase under control promoter. High constitutive transgene expression was found small intestine only, whereas little reporter activity observed other tissues. Within bowel, promoter restricted to...
IL-12 p40–related cytokines such as p35/p40 heterodimer and IL-23 (p19/p40) are potent regulators of adaptive immune responses. Little is known, however, about the transcriptional regulation p40 gene in vivo. In an attempt toward this goal, we have generated transgenic mice expressing firefly luciferase under control promoter. High constitutive transgene expression was found small intestine only, whereas little reporter activity observed other tissues. Within bowel, promoter restricted to...
Bartonella henselae causes vasculoproliferative disorders in humans. We identified a nonfimbrial adhesin of B. designated as A (BadA). BadA is 340-kD outer membrane protein encoded by the 9.3-kb badA gene. It has modular structure and contains domains homologous to Yersinia enterocolitica (Yersinia A). Expression was restored BadA-deficient transposon mutant complementation trans. mediates binding extracellular matrix proteins endothelial cells, possibly via β1 integrins, but prevents...
Abstract IL-12-induced IFN-γ production is essential for clearance of Yersinia enterocolitica infection. Similar to IL-12, the recently described cytokine IL-18 (IFN-γ-inducing factor) produced by macrophages and induces in spleen cells. Therefore, we have investigated role infection mice. Heat-killed yersinia-triggered IL-18-promoted splenocytes was predominantly dependent on endogenous IL-12 production, whereas IL-12-promoted not dependent. IL-18-induced a higher degree IFN-γR-mediated...
Yersinia enterocolitica is enteropathogenic for man and rodents. Previous studies provided evidence that Y. invades the lymphoid follicles of Peyer's patches (PP) small intestine. In this study enterocolitica-induced tissue alterations follicle-associated epithelium (FAE) underlying PP were analysed by scanning (SEM) transmission electron microscopy (TEM) as well conventional histological examination. For purpose, an experimental mouse infection model including orogastric infections ileal...
Susceptibility of mice to infection with Yersinia enterocolitica has been shown be related neither the Ity locus encoding for resistance Salmonella typhimurium and other pathogens nor H-2 locus. Recent studies in our laboratory have demonstrated that T-cell-mediated immune responses are required overcoming primary infection. In present study, we investigated course Y. resulting Yersinia-susceptible BALB/c Yersinia-resistant C57BL/6 mice. early phase infection, clearance pathogen was...
The spread of Gram-negative bacteria with plasmid-borne extended-spectrum beta-lactamases (ESBLs) has become a worldwide problem. This study analysed total 366 ESBL-producing Enterobacteriaceae strains isolated from non-selected patient specimens at the university hospital Tübingen in period January 2003 to December 2007. Although overall ESBL rate was comparatively low (1.6 %), percentages Enterobacter spp. and Escherichia coli increased 0.8 0.5 %, respectively, 4.6 3.8 % In particular,...
Bloodstream infections (BSI) are an important cause of morbidity and mortality. Increasing rates antimicrobial-resistant pathogens limit treatment options, prompting empirical use broad-range antibiotics. Fast reliable diagnostic tools needed to provide adequate therapy in a timely manner enable de-escalation treatment. The Accelerate Pheno system (Accelerate Diagnostics, USA) is fully automated test that performs both identification antimicrobial susceptibility testing (AST) directly from...
The selection pressure exercised by antibiotic drugs is an important consideration for the wise stewardship of antimicrobial treatment programs. Treatment decisions are currently based on crude assumptions, and there urgent need to develop a more quantitative knowledge base that can enable predictions impact individual antibiotics human gut microbiome resistome.Using shotgun metagenomics, we quantified changes in two cohorts hematological patients receiving prophylactic antibiotics; one...
Nasal colonization is a major risk factor for S. aureus infections. The mechanisms responsible are still not well understood and involve several factors on the host bacterial side. One key cell wall teichoic acid (WTA) of aureus, which governs direct interactions with nasal epithelial surfaces. We report here first receptor glycopolymer WTA cells. In assay systems this type F-scavenger receptor, termed SREC-I, bound in charge dependent manner mediated adhesion to cells vitro. impact SREC-I...
Infections with multidrug-resistant bacteria often leave limited or no treatment options. The transfer of antimicrobial resistance genes (ARG) carrying plasmids between bacterial species by horizontal gene represents an important mode expansion ARGs. Here, we demonstrate the application Nanopore sequencing in a hospital setting for monitoring and rapid evolution antibiotic within across multiple species. In 2009, experienced outbreak extensively Pseudomonas aeruginosa harboring...
Bartonella henselae causes the vasculoproliferative disorders bacillary angiomatosis (BA) and peliosis (BP). The pathomechanisms of these tumorous proliferations are unknown. Our results suggest a novel bacterial two-step pathogenicity strategy, in which pathogen triggers growth factor production for subsequent proliferation its own host cells. In fact, B. induces cell angiogenic vascular endothelial (VEGF), leading to presence pili was associated with VEGF production, as Pil− mutant unable...
Background— Bartonella species are the only known bacterial pathogens causing vasculoproliferative disorders in humans (bacillary angiomatosis [BA]). Cellular and pathogenetic mechanisms underlying induction of BA largely unknown. Methods Results— Activation hypoxia-inducible factor-1 (HIF-1), key transcription factor involved angiogenesis, was detected henselae –infected host cells vitro by immunofluorescence, Western blotting, electrophoretic mobility shift, reporter gene assays...
ABSTRACT Invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. Conventional identification pathogenic fungi clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation an adequate antifungal therapy. We developed diagnostic microarray rapid simultaneous 12 most common Candida Aspergillus species. Oligonucleotide probes were designed by exploiting sequence variations internal...