A5027979430- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Vibrio bacteria research studies
- Gut microbiota and health
- Cancer Research and Treatments
- Pharmacological Effects of Natural Compounds
- Clostridium difficile and Clostridium perfringens research
- Yersinia bacterium, plague, ectoparasites research
- Immune Response and Inflammation
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Digestive system and related health
- Cancer Cells and Metastasis
- Diet and metabolism studies
- Helicobacter pylori-related gastroenterology studies
- Dietary Effects on Health
- Mycobacterium research and diagnosis
- RNA Interference and Gene Delivery
- Natural product bioactivities and synthesis
- Circadian rhythm and melatonin
- Bartonella species infections research
- Gastrointestinal motility and disorders
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
Medizinische Hochschule Hannover
2015-2024
German Center for Infection Research
2015-2024
Kiel University
2014-2020
Research Center Borstel - Leibniz Lung Center
2014-2019
University of British Columbia
2006-2015
Canada's Michael Smith Genome Sciences Centre
2006-2012
Upsher-Smith Laboratories (United Kingdom)
2006-2010
Zero to Three
2006
Institute of Medical Microbiology and Hygiene
1999-2006
Universitätsklinikum Tübingen
2003-2006
The intestinal microbiota is involved in many physiological processes and it increasingly recognized that differences community composition can influence the outcome of a variety murine models used biomedical research. In an effort to describe account for variation across animal facilities participating members DFG Priority Program 1656 "Intestinal Microbiota", we performed survey C57BL/6J mice from 21 different mouse rooms/facilities located at 13 institutions Germany. Fresh feces was...
Abstract Recent evidence suggests that natural selection operating on hosts to maintain their microbiome contributes the emergence of new species, is, ‘hologenomic basis speciation’. Here we analyse gut microbiota two house mice subspecies, Mus musculus and M. m. domesticus , across Central European hybrid zone, in addition hybrids generated lab. Hybrid display widespread transgressive phenotypes (that exceed or fall short parental values) a variety measures bacterial community structure,...
Abstract Mycobacterium tuberculosis ( Mtb ) uses alveolar macrophages as primary host cells during infection. In response to an infection, switch from pyruvate oxidation reduction of into lactate. Lactate might present additional carbon substrate for . Here, we demonstrate that can utilize L-lactate sole source in vitro growth. conversion is strictly dependent on one two potential dehydrogenases. A knock-out mutant lacking lldD2 (Rv1872c) was unable L-lactate. contrast, the lldD1 (Rv0694)...
The long external filament of bacterial flagella is composed several thousand copies a single protein, flagellin. Here, we explore the role played by lysine methylation flagellin in Salmonella, which requires methylase FliB. We show that both flagellins Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed residues A Typhimurium mutant deficient outcompeted for gut colonization gastroenteritis mouse model, promotes invasion epithelial cells vitro. Lysine...
Background— Bartonella species are the only known bacterial pathogens causing vasculoproliferative disorders in humans (bacillary angiomatosis [BA]). Cellular and pathogenetic mechanisms underlying induction of BA largely unknown. Methods Results— Activation hypoxia-inducible factor-1 (HIF-1), key transcription factor involved angiogenesis, was detected henselae –infected host cells vitro by immunofluorescence, Western blotting, electrophoretic mobility shift, reporter gene assays...
Expression of ABO and Lewis histo-blood group antigens by the gastrointestinal epithelium is governed an α-1,2-fucosyltransferase enzyme encoded Fut2 gene. Alterations in mucin glycosylation have been associated with susceptibility to various bacterial viral infections. Salmonella enterica serovar Typhimurium a food-borne pathogen major cause gastroenteritis. In order determine role Fut2-dependent glycans Salmonella-triggered intestinal inflammation, Fut2+/+ Fut2-/- mice were orally infected...
ABSTRACT The Salmonella rdar ( r ed, d ry, a nd ough) morphotype is an aggregative and resistant physiology that has been linked to survival in nutrient-limited environments. Growth of enterica serovar Typhimurium was analyzed variety nutrient-limiting conditions determine whether aggregation would occur at low cell densities the involved this process. resulting cultures consisted two populations cells, aggregated nonaggregated, with cells preferentially displaying gene expression. groups...
Glycans play important roles in host-microbe interactions. Tissue-specific expression patterns of the blood group glycosyltransferase β-1,4-N-acetylgalactosaminyltransferase 2 (B4galnt2) are variable wild mouse populations, and loss B4galnt2 is associated with altered intestinal microbiota. We hypothesized that variation alters susceptibility to pathogens. To test this, we challenged mice genetically engineered express different tissue-specific a Salmonella Typhimurium infection model. found...
Subcellular compartmentalization of receptor signaling is an emerging principle in innate immunity. However, the functional integration pathways into membrane trafficking routes and its physiological relevance for immune responses still largely unclear. In this study, using Lyst-mutant beige mice, we show that lysosomal regulator Lyst links endolysosomal organization to selective control toll-like 3 (TLR3)- TLR4-mediated proinflammatory responses. Consequently, mice showed increased...
Basic processes of the fatty acid metabolism have an important impact on function intestinal epithelial cells (IEC). However, while role cellular oxidation is well appreciated, it not clear how de novo synthesis (FAS) influences biology IECs. We report here that interfering with FAS by deletion enzyme Acetyl-CoA-Carboxylase (ACC)1 in IECs results loss crypt structures and a specific decline Lgr5+ stem (ISC). Mechanistically, ACC1-mediated supports formation organoids differentiation complex...
The Yersinia outer surface protein invasin binds to beta1 integrins on target cells and has been shown trigger phagocytic uptake by macrophages. Here, we investigated the role of actin regulator Wiskott-Aldrich syndrome (WASp), its effector Arp2/3 complex Rho-GTPases CDC42Hs, Rac Rho in invasin/beta1 integrin-triggered phagocytosis. During invasin-coated latex beads, alpha5beta1 integrin, WASp were recruited developing actin-rich cups primary human Blockage specific antibodies, inhibition...
A recently developed model for enterocolitis in mice involves pre-treatment with the antibiotic streptomycin prior to infection Salmonella enterica serovar Typhimurium (S. Typhimurium). The contribution of Nramp1/Slc11a1 protein, a critical host defence mechanism against S. Typhimurium, development inflammation this has not been studied. Here, we analysed impact Nramp1 expression on early colitis using isogenic Nramp1(+/+) and Nramp1(-/-) mice. We hypothesized that acts by rapidly inducing...
Enteropathogenic Yersinia bacteria trigger the production of proinflammatory chemokine IL-8, an important for recruitment polymorphonuclear leukocytes (PMN). is resistant to phagocytosis by PMN, and these cells thought be part a pathogenic strategy establish infection allowing pathogen gain access to, disseminate within, host tissue. We report here that expressing outer membrane protein invasin triggers IL-8 in epithelial cells. The 195 carboxyl-terminal amino acids when linked latex beads...
ABSTRACT The major invasive factor of Yersinia enterocolitica , the invasin (Inv) protein, induces proinflammatory host cell responses, including interleukin-8 (IL-8) secretion from human epithelial cells, by engagement β1 integrins. Inv-triggered integrin signaling involves small GTPase Rac; activation MAP kinases, such as p38, MEK1, and JNK; transcription NF-κB. In present study, we demonstrate that Y. YadA, which is a adhesin with pleiotropic virulence effects, IL-8 in cells. abilites...
Gastrointestinal infectious diseases remain an important issue for human and animal health. Investigations on gastrointestinal are classically performed in laboratory animals leading to the problem that species-specific models scarcely available, especially when it comes farm animals. The 3R principles of Russel Burch were achieved using intestinal organoids porcine jejunum. These seem be a promising tool generate vitro epithelium. 3D Organoids grown extracellular matrix characterized by...