A5082288933- Advanced Proteomics Techniques and Applications
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Protein purification and stability
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Colorectal Cancer Surgical Treatments
- Venous Thromboembolism Diagnosis and Management
- Biomedical Text Mining and Ontologies
- Healthcare during COVID-19 Pandemic
- Immune cells in cancer
- 14-3-3 protein interactions
- Biosimilars and Bioanalytical Methods
- Analytical Chemistry and Chromatography
- Biosensors and Analytical Detection
- Retinoids in leukemia and cellular processes
- Endoplasmic Reticulum Stress and Disease
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Prostate Cancer Treatment and Research
- Heat shock proteins research
- Erythrocyte Function and Pathophysiology
- Diabetes Management and Education
University Medical Center Hamburg-Eppendorf
2015-2024
Universität Hamburg
2015-2024
Eppendorf (Belgium)
2024
The University of Sydney
2020-2023
Grupo Hospitalar Conceição
2020
Macquarie University
2018-2020
Australian Genome Research Facility
2018
University Cancer Center Hamburg
2017
The long external filament of bacterial flagella is composed several thousand copies a single protein, flagellin. Here, we explore the role played by lysine methylation flagellin in Salmonella, which requires methylase FliB. We show that both flagellins Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed residues A Typhimurium mutant deficient outcompeted for gut colonization gastroenteritis mouse model, promotes invasion epithelial cells vitro. Lysine...
Microsampling of patient blood promises several benefits over conventional phlebotomy practices to facilitate precision medicine studies. These include at-home collection, supporting telehealth monitoring, minimal postcollection processing, and compatibility with nonrefrigerated transport storage. However, for proteomic biomarker studies, mass spectrometry whole has generally been avoided in favor using plasma or serum obtained from venepuncture. We evaluated the use a volumetric absorptive...
Posttranslational modifications and proteolytic processing regulate almost all physiological processes. Dysregulation can potentially result in pathologic protein species causing diseases. Thus, tissue proteomes of diseased individuals provide diagnostic information. Since the composition rapidly change during homogenization by action enzymes released from their compartments, disease specific patterns vanish. Recently, we described a novel, ultrafast soft method for cold vaporization via...
Cosmc is ubiquitously expressed and acts as a specific molecular chaperone assisting the folding stability of core 1 synthase. Thus, it plays crucial role in biosynthesis O-linked glycosylation proteins. Here, we show that ablation exocrine pancreas mice causes expression truncated O-glycans (Tn antigen), resulting pancreatic insufficiency with decreased activities digestive enzymes diabetes. To understand pleiotropic phenotype, used Vicia villosa agglutinin to enrich Tn antigen-modified...
The complexity of mammalian proteomes is a challenge in bottom-up proteomics. For comprehensive proteome analysis, multidimensional separation strategies are necessary. Online two-dimensional liquid chromatography–tandem mass spectrometry (2D-LC-MS/MS) combining strong cation exchange (SCX) the first dimension with reversed-phase (RP) chromatography second provides powerful approach to analyze complex proteomes. Although combination SCX RP good orthogonality, only moderate achieved for...
Histological risk factors for lymph node metastasis (LNM) in early-stage colorectal cancers (CRC) have been described, although the predictive utility of these varies. Improved LNM assessment based on findings endoscopic colon and rectal excisions is necessary optimal surgical management CRC patients with pathologic T1- /T2-staged invasive depth (i.e., tumor not invading beyond muscularis propria layer); as current system overly conservative, results many unnecessary radical surgeries. To...
BackgroundDistant metastasis formation is the major clinical problem in prostate cancer (PCa) and underlying mechanisms remain poorly understood. Our aim was to identify novel molecules that functionally contribute human PCa systemic dissemination based on unbiased approaches.MethodsWe compared mRNA, microRNA (miR) protein expression levels established xenograft tumours with high (PC-3), moderate (VCaP) or weak (DU-145) spontaneous micrometastatic potential. By focussing those mRNAs, miRs...
Early T stage colorectal cancers (CRC) that invade lymph nodes (Stage IIIA) are rare and greatly under-represented in large-scale genomic mapping projects. We retrieved 10 Stage IIIA CRC cases, matched these to 16 1 cases (T1 depth without node metastasis) carried out deep sequencing of 409 genes using the IonTorrent system. Tumour mutational burdens (TMB) ranged from 2.4 77.2/Mb sequenced. No stage-related differences were observed, consistent with reanalysis The Cancer Genome Atlas (TCGA)...
Proteomics and genomics discovery experiments generate increasingly large result tables, necessitating more researcher time to convert the biological data into new knowledge. Literature review is an important step in this process can be tedious for scale experiments. An informed strategic decision about which biomolecule targets should pursued follow-up thus remains a considerable challenge. To streamline formalise of literature retrieval analysis based ‘omics as decision-facilitating...
Rickettsioses are neglected and emerging potentially fatal febrile diseases that caused by obligate intracellular bacteria, rickettsiae. Rickettsia ( R .) typhi . prowazekii constitute the typhus group (TG) of rickettsiae causative agents endemic epidemic typhus, respectively. We recently generated a monoclonal antibody (BNI52) against Characterization BNI52 revealed it specifically recognizes TG but not members spotted fever (SFG) further show binds to protein fragments ±30 kDa exposed on...
Abstract: A continuously increasing number of therapeutic proteins are being released into the market, including biosimilars. In contrast to small organic drugs, require an extensive analysis their exact chemical composition because complexity and proof absence contaminants, such as host cell nucleic acids. Especially challenging is detection low abundant species these usually very similar target protein. However, important for safety patients a change may cause serious side effects. this...
The combination of radiotherapy and pharmacological inhibition cellular signal transduction pathways offers promising strategies for enhanced cancer cell inactivation. However, the molecular effects kinase inhibitors especially on DNA damage detection repair after X-irradiation have to be understood facilitate development efficient personalized treatment regimens. Therefore, we applied differential proteomics analyzing inhibitor-induced changes in either chromatin-bound or phosphorylated...
<title>Abstract</title> Background Genome instability is a fundamental feature and hallmark of cancer associated with aggressiveness, drug resistance poor prognosis. RAI2 was initially identified as novel metastasis suppressor protein specifically the presence disseminated tumour cells in bone marrow breast patients, but its molecular function largely unknown. Methods We analysed consequences depletion on gene expression genomic stability luminal cell lines, performed cytotoxicity profiling...
Approximately 20% of locally advanced rectal cancer (LARC) patients treated preoperatively with chemoradiotherapy (CRT) achieve pathologically confirmed complete regression. However, there are no clinically implemented biomarkers measurable in biopsies that predictive tumor Here, we conducted multiplexed immunophenotyping diagnostic from 16 LARC CRT. We identified greater regression had higher infiltration pan-T cells and IRF8+HLA-DR+ prior to High cell density was further associated...
Abstract Early T stage colorectal cancers (CRC) that invade lymph nodes (Stage IIIA) are greatly under-represented in large-scale genomic mapping projects such as TCGA datasets. We retrieved 10 Stage IIIA CRC cases, matched these to 16 1 cases (T1 depth without node metastasis) and carried out deep sequencing of 409 genes using the IonTorrent system. Tumour mutational burdens (TMB) ranged from 2.4-77.2/Mb sequenced. Using mean TMB a cut-point define groups, TMB-low (TMB-L) specimens showed...
Abstract We have identified low RAI2 expression as a novel metastasis-associated genetic alteration especially related to the presence of disseminated tumor cells in bone marrow breast cancer patients and poor outcome (Werner et al, Cancer Discovery, 2015). In hormone-dependent prostate cell lines depletion induces down-regulation hormone receptor expression. Furthermore, is associated with chromosomal instability. Nevertheless, date exact molecular mechanisms behind these observations...
Urotensin II (UII) and UII-related peptide (URP) are vasoactive hormones causing strong vasoconstriction or vasodilation, depending on the type of blood vessel. In humans, active forms resulting from proteolytic cleavage their inactive precursor protein. plasma, a defined protease converting UII URP precursors into has not been identified yet. Using mass spectrometry-based enzyme screening for detecting UII- URP-converting enzymes, human plasma fraction Cohn IV-4 was chromatographed,...
Abstract The flagellum is the motility device of many bacteria and long external filament made several thousand copies a single protein, flagellin. While posttranslational modifications flagellin are common among bacterial pathogens, role lysine methylation remained unknown. Here, we show that both flagellins Salmonella enterica , FliC FljB, methylated at surface-exposed residues. A mutant deficient in was outcompeted for gut colonization gastroenteritis mouse model. In support, promoted...
Abstract Background Motivational Interviewing (MI) has been effective in promoting positive changes various health-related behaviors, including chronic disease management. The main goal of this study was evaluating the effectiveness care technology individual nursing consultations for control type 2 Diabetes Mellitus (2DM) and Arterial Hypertension (AH) primary healthcare. Methods Double-blind, controlled, parallel randomized clinical trial conducted Porto Alegre, Rio Grande do Sul - Brazil,...
<h3>Introduction</h3> Successful reperfusion (mTICI2c/3) and a low number of passes are key determinants for good clinical outcome in acute stroke patients<sup>1</sup> since the retrieval attempts correlate negatively with functional outcome.<sup>2</sup> Final mTICI2c/3 is superior to final mTICI2b(3), but it remains unclear if this true subgroup patients early (≤2 attempts) mTICI2b who secondarily improved mTICI2c/3. <h3>Aim Study</h3> The goal study was analyse benefit clinically when...