A5012148121- Glycosylation and Glycoproteins Research
- Blood groups and transfusion
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Dental Implant Techniques and Outcomes
- Cancer, Hypoxia, and Metabolism
- Helicobacter pylori-related gastroenterology studies
- Bone Tissue Engineering Materials
- Receptor Mechanisms and Signaling
- Ubiquitin and proteasome pathways
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Cancer Cells and Metastasis
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Neurobiology and Insect Physiology Research
- Dental materials and restorations
- Erythrocyte Function and Pathophysiology
- Protein Kinase Regulation and GTPase Signaling
- RNA and protein synthesis mechanisms
- Venomous Animal Envenomation and Studies
University Medical Center Hamburg-Eppendorf
2014-2025
Universität Hamburg
2014-2025
University Hospital Schleswig-Holstein
2018-2019
University of Lübeck
2018-2019
University of Göttingen
2010
Eppendorf (Germany)
1999-2000
Human pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies in world despite great efforts research types treatment remain limited. A frequently detected alteration PDACs a truncated O-linked N-acetylgalactosamine (GalNAc) glycosylation with expression Tn antigen. Changes O-glycosylation affect posttranslationally modified O-GalNAc proteins resulting profound cellular alterations. antigen tumor associated glycan 75-90 % up to 67 its precursor lesions....
Abstract Cytotoxic stress activates stress-activated kinases, initiates adaptive mechanisms, including the unfolded protein response (UPR) and autophagy, induces programmed cell death. Fatty acid unsaturation, controlled by stearoyl-CoA desaturase (SCD)1, prevents cytotoxic but mechanisms are diffuse. Here, we show that 1,2-dioleoyl- sn -glycero-3-phospho-(1’-myo-inositol) [PI(18:1/18:1)] is a SCD1-derived signaling lipid, which inhibits p38 mitogen-activated kinase activation, counteracts...
By combining a Drosophila genome data base search and reverse transcriptase-PCR-based cDNA isolation, two G-protein-coupled receptors were cloned, which are the closest known invertebrate homologs of mammalian opioid/somatostatin receptors. However, when functionally expressed in Xenopus oocytes by injection orphan receptor RNAs together with coexpressed potassium channel, neither was activated agonists. applying pharmacological approach, physiological ligands isolated from peptide extracts...
Metastases arise from disseminated tumor cells (DTC) that colonize secondary organs. However, DTC survival strategies to start metastatic outgrowth are unclear. The hostile (hypoxic, hypoglycemic) microenvironmental conditions of the bone marrow serve as an ideal model environment for investigation under environmental stress. We investigated breast cancer cell line BC-M1 established a patient by 2-D DIGE and MS analysis. observed specific overexpression unfolded protein response (UPR)...
Abstract Aims Reduced left atrial PITX2 is associated with cardiomyopathy and fibrillation (AF). restricted to cardiomyocytes (aCMs) in the adult heart. The links between deficiency, cardiomyopathy, AF are not fully understood. Methods results To identify mechanisms linking deficiency AF, we generated characterized PITX2-deficient human aCMs derived from induced pluripotent stem cells (hiPSC) their controls. hiPSC-derived showed shorter disorganized sarcomeres increased mononucleation....
Abstract Cell death programs such as apoptosis and ferroptosis are associated with aberrant redox homeostasis linked to lipid metabolism membrane function. Evidence for cross-talk between these is emerging. Here, we show that cytotoxic stress channels polyunsaturated fatty acids via lysophospholipid acyltransferase 12 into phospholipids become susceptible peroxidation under additional stress. This reprogramming altered acyl-CoA synthetase isoenzyme expression caused by a decrease in growth...
TFF3 is a typical secretory poplypeptide of mucous epithelia belonging to the trefoil factor family (TFF) lectins. In intestine, respiratory tract, and saliva, mainly exists as high-molecular-mass complex with IgG Fc binding protein (FCGBP), which indicative role in mucosal innate immunity. For first time, we identified different forms endocervix, i.e., monomeric homodimeric TFF3, well TFF3-FCGBP complex; latter also hardly soluble form. Immunohistochemistry co-localized FCGBP. Expression...
Genes implicated in translation control have been associated with autism spectrum disorders (ASDs). However, some important genetic causes of autism, including the 16p11.2 microdeletion, bear no obvious connection to translation. Here, we use proteomics, genetics, and assays cultured cells mouse brain reveal altered mediated by loss kinase TAOK2 deletion models. We show that associates translational machinery functions as a brake phosphorylating eukaryotic elongation factor 2 (eEF2)....
Dissemination of primary cancer cells to distant sites is an early event in breast cancer. These can invade the bone marrow, rest there, and many years later disseminated tumor (DTC) grow out form overt metastases. Epithelium specific cytokeratins are commonly used as marker proteins for sensitive detection metastatic lesions. However, due difficulties DTC, question arises if DTC necessarily have same protein expression profile advanced tumors. On that account, we analyzed previously...
Abstract Mucous gels (mucus) cover internal body surfaces. The secretory mucins MUC5AC and MUC6 the protective peptide TFF2 are characteristic constituents of gastric mucus; is co‐secreted with MUC6. Herein, we investigated two commercial mucin preparations by FPLC proteomics, because they model systems for studying rheology mucins. One preparation also used as a saliva substitute, example, after radiation therapy. We show that both contain (≈0.6 to 1.1 %, w / ). majority strongly bound...
The peptide TFF3 is a member of family secretory lectins, and typically synthesized by mucous epithelia together with mucins. It mainly released from intestinal goblet cells as high-molecular mass heterodimer IgG Fc binding protein (FCGBP). Herein, we investigated human saliva fast liquid chromatography (FPLC) proteomics identified high- low-molecular-mass forms TFF3. Whereas the high-molecular-mass represent FCGBP, homodimeric forms. Proteomic analysis also revealed C-terminally truncated...
TFF1 is a protective peptide of the Trefoil Factor Family (TFF), which co-secreted with mucin MUC5AC, gastrokine 2 (GKN2), and IgG Fc binding protein (FCGBP) from gastric surface mucous cells. Tff1-deficient mice obligatorily develop antropyloric adenoma about 30% progress to carcinomas, indicating that Tff1 tumor suppressor. As hallmark, contains seven cysteine residues three disulfide bonds stabilizing conserved TFF domain. Here, we systematically investigated molecular forms in human...
The ABO gene locus is associated with the risk of developing pancreatic ductal adenocarcinoma (PDAC) resulting in an increased incidence individuals non-O blood groups. Up to 90% PDAC specimens display alterations mucin type O-GalNAc glycosylation. Because aberrant glycans (Tn and T antigen) are structurally related group A B glycans, we investigated role IgM isoagglutinins PDAC.Binding studies toward A, B, Tn antigen, antigen glycoconjugates from patients healthy were conducted....
Elapid snake venom is a highly valuable, but till now mainly unexplored, source of pharmacologically important peptides. We analyzed the peptide fractions with molecular masses up to 10 kDa two elapid venoms-that African cobra, N. m. mossambica (genus Naja), and Peninsula tiger snake, scutatus, from Kangaroo Island Notechis). A combination chromatographic methods was used isolate peptides, which were characterized by combining complimentary mass spectrometric techniques. Comparative analysis...
Cosmc is ubiquitously expressed and acts as a specific molecular chaperone assisting the folding stability of core 1 synthase. Thus, it plays crucial role in biosynthesis O-linked glycosylation proteins. Here, we show that ablation exocrine pancreas mice causes expression truncated O-glycans (Tn antigen), resulting pancreatic insufficiency with decreased activities digestive enzymes diabetes. To understand pleiotropic phenotype, used Vicia villosa agglutinin to enrich Tn antigen-modified...
A prerequisite for the transition toward a biobased economy is identification and development of efficient enzymes usage renewable resources as raw material. Therefore, different xylanolytic are important enzymatic hydrolysis xylan-heteropolymers. powerful tool to overcome limited toolbox lies in exhausting potential unexplored habitats. By screening Vietnamese fungal culture collection 295 undiscovered isolates, 12 highly active xylan degraders were identified. One best xylanase producing...
Snake venom peptidomes are valuable sources of pharmacologically active compounds. We analyzed the peptidic fractions (peptides with molecular masses < 10 000 Da) venoms Vipera ammodytes meridionalis (Viperinae), most toxic snake in Europe, and Bothrops jararacussu (Crotalinae), an extremely poisonous South America. Liquid chromatography/mass spectrometry (LC/MS), direct infusion electrospray mass (ESI-MS) matrix-assisted desorption/ionization time-of-flight (MALDI-TOF-MS) were applied to...
Abstract Background Neurodevelopmental disorders such as autism spectrum disorder (ASD) may be caused by alterations in genes encoding proteins that are involved synapse formation and function. This includes scaffold Shank3, synaptic adhesion Neurexins or Neuroligins. An important question is whether the products of individual risk cooperate functionally (exemplified interaction Neurexin with Neuroligin isoforms). might suggest a common pathway pathogenesis. For SHANK3 gene, heterozygous...
Abstract Purpose: The monoclonal antibody Ki-A10 (IgG1) generated after immunization of mice with Hodgkin's lymphoma cell line L428 detects a nuclear antigen in human tissues restricted distribution pattern similar to cancer/testis antigens. aim this study was characterize the and determine expression profile lymphoma. Experimental Design: half-life phosphorylation were determined by radiolabeling. characterized immunopurification sequencing. Demethylation genes is used induce...
Lactate is a universal metabolite and energy source, yet the mode of lactate metabolism in many strictly anaerobic microorganisms still enigmatic. This sparked us to investigate biochemistry bioenergetics model acetogenic bacterium Moorella thermoacetica. Growth were dependent on CO2 chemiosmotic gradient. We discovered l-lactate:NAD+ oxidoreductase (LDH) cell-free extracts, exhibiting an average specific activity 362.8 ± 22.9 mU mg-1 . The enzyme was reversible, most active at 65°C pH 9,...
Protein biomarker discovery in the low concentration range of human body fluids requires enrichment proteins interest. Here we report on a tandem affinity strategy: In first step, isolated plasma glyco-subproteome healthy individuals by wheat germ agglutinin (WGA) lectin chromatography. second this subproteome were used to raise antibodies llama (Lama glama). The heavy-chain fraction was deplete from WGA binding all normally found plasma. way, selectively enriched glycoprotein, CEA, known...
Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production scavenging enzymes in plants. The influence hypoxia on roots hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq RT-qPCR) proteome (LC–MS/MS 2D-PAGE) analyses were used determine the alterations soluble membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase plasma proteins showed...