A5013723014- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Antifungal resistance and susceptibility
- Probiotics and Fermented Foods
- Infective Endocarditis Diagnosis and Management
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Microbial Metabolism and Applications
- Genomics and Phylogenetic Studies
- Antibiotic Use and Resistance
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Salmonella and Campylobacter epidemiology
- Urinary Tract Infections Management
- Immunodeficiency and Autoimmune Disorders
- Bacillus and Francisella bacterial research
- Plant Pathogenic Bacteria Studies
Robert Koch Institute
2012-2025
B. Braun (Germany)
2022
Johannes Gutenberg University Mainz
2021
Friedrich Schiller University Jena
2019
Federal Ministry of Education and Research
2009
Society for Healthcare Epidemiology of America
2009
Medizinische Hochschule Hannover
2009
Charité - Universitätsmedizin Berlin
2009
GTx (United States)
2004
Humboldt-Universität zu Berlin
1988-2002
ABSTRACT The spa gene of Staphylococcus aureus encodes protein A and is used for typing methicillin-resistant (MRSA). We sequence the repeat region to study epidemiology MRSA at a German university hospital. One hundred seven 84 strains were studied during two periods 10 4 months, respectively. Repeats types determined by Ridom StaphType, novel software tool allowing rapid determination, data management retrieval, Internet-based assignment new following automatic quality control DNA...
Besides hospitals animal husbandry represents a large reservoir of transferable antibiotic resistance genes. Via food products resistant bacteria can easily reach humans. The current debate is above all concerning use as growth promoters which be gradually phased out by better performance.
Pulsed-fieldgel electrophoresis (PFGE) is the most common genotypic method used in reference and clinical laboratories for typing methicillin-resistant Staphylococcus aureus (MRSA). Many different protocols have been developed that extensive experience with technique established national databases. However, comparabilities of European PFGE MRSA various clones themselves had not addressed until now. This multinational Union (EU) project has first time a database representative epidemic...
In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes Staphylococcus aureus. Conditions were optimized to amplify fragments mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside tetK, tetM (tetracycline erm(A), erm(C) (macrolide-lincosamide-streptogramin B vat(A), vat(B), and vat(C) (streptogramin A resistance) simultaneously in one amplification. An additional primer pair amplification fragment staphylococcal...
Abstract We explored the variation in proportions of methicillin-resistant Staphylococcus aureus (MRSA) between and within countries participating European Antimicrobial Resistance Surveillance System temporal trends its occurrence. This system collects routine antimicrobial susceptibility tests for S. aureus. examined data collected from January 1999 through December 2002 (50,759 isolates 495 hospitals 26 countries). MRSA prevalence varied almost 100-fold, <1% northern Europe to >40%...
Nowadays, six types of acquired vancomycin resistance in enterococci are known; however, only VanA and to a lesser extent VanB widely prevalent. Various genes encode these typically associated with mobile genetic elements which allow spread clonally laterally. The major reservoir is Enterococcus faecium; vancomycin-resistant faecalis still rare. Population analysis E. faecium has revealed distinct subpopulation hospital-acquired strain types, can be differentiated by molecular typing methods...
The widespread use of antibiotics in association with high-density clinical care has driven the emergence drug-resistant bacteria that are adapted to thrive hospitalized patients. Of particular concern globally disseminated methicillin-resistant Staphylococcus aureus (MRSA) clones cause outbreaks and epidemics associated health care. most rapidly spreading tenacious health-care-associated clone Europe currently is EMRSA-15, which was first detected UK early 1990s subsequently spread...
Abstract Methicillin-resistant Staphylococcus aureus of clonal lineage ST398 that exhibits related spa types and contains SCCmec elements IVa or V has been isolated from colonized infected humans companion animals (e.g., dog, pig, horse) in Germany Austria. Of particular concern is the association these cases with nosocomial ventilator-associated pneumonia.
To determine MICs of 16 antimicrobials representing all major classes for 473 taxonomically well-characterized isolates lactic acid bacteria (LAB) encompassing the genera Lactobacillus, Pediococcus and Lactococcus. propose tentative epidemiological cut-off (ECOFF) values recognizing intrinsic acquired antimicrobial resistances in numerically dominant species. On basis depositors' information, LAB were grouped categories probiotic, nutritional, probiotic or nutritional research, human animal...
Abstract The discovery of antibiotics more than 80 years ago has led to considerable improvements in human and animal health. Although antibiotic resistance environmental bacteria is ancient, pathogens thought be a modern phenomenon that driven by the clinical use 1 . Here we show particular lineages methicillin-resistant Staphylococcus aureus —a notorious pathogen—appeared European hedgehogs pre-antibiotic era. Subsequently, these spread within local hedgehog populations between secondary...
ABSTRACT The implementation of routine whole-genome sequencing (WGS) promises to transform our ability monitor the emergence and spread bacterial pathogens. Here we combined WGS data from 308 invasive Staphylococcus aureus isolates corresponding a pan-European population snapshot, with epidemiological resistance data. Geospatial visualization is made possible by generic software tool designed for public health purposes that available at project URL (...
Biofilm production is an important step in the pathogenesis of Staphylococcus epidermidis polymer-associated infections and depends on expression icaADBC operon leading to synthesis a polysaccharide intercellular adhesin. A chromosomally encoded reporter gene fusion between ica promoter beta-galactosidase lacZ from Escherichia coli was constructed used investigate influence both environmental factors subinhibitory concentrations different antibiotics S. epidermidis. It shown that biofilm...
Glycopeptide-resistant Enterococcus faecium strains were isolated from a pig farm and poultry both using avoparcin as food additive. Such organisms not in hen's eggs-producing avoparcin. enterococci also detected broiler chicken carcasses that delivered to hospital's kitchen. The resistance was determined by the vanA gene indicated detection of inducible 39-kDa cytoplasmic membrane protein vanA-specific DNA sequence amplified polymerase chain reaction. Genomic fragment patterns animal...
The use of the glycopeptide antibiotic avoparcin (AVO) as a feed additive in animal husbandry many European countries led 1994-1995 to frequent isolation VanA-type vancomycin-resistant enterococci (VRE) from commercially produced foodstuffs well fecal samples nonhospitalized persons Germany (Saxony-Anhalt state). However, at end 1997, decreasing number such VRE was detected frozen and fresh poultry meat (chickens turkeys) German producers. At this point time, AVO had been discontinued for...
The implementation of the new clustering algorithm Based Upon Repeat Pattern (BURP) into Ridom StaphType software tool enables based on spa typing data for Staphylococcus aureus. We compared results obtained by typing/BURP to those currently well-established methods, i.e., SmaI macrorestriction analysis and multilocus sequence typing/eBURST. A total 99 clinical S. aureus strains, including MRSA representing major clonal lineages associated with important kinds infections which have been...
A small number of clonal lineages dominates the global population structure methicillin-resistant Staphylococcus aureus (MRSA), resulting in concept that MRSA has emerged on a few occasions after penicillinase-stable beta-lactam antibiotics were introduced to clinical practice, followed by intercontinental spread individual clones. We investigated evolutionary history an clone (ST5) mutation discovery at 108 loci (46 kb) within collection 135 isolates. The SNPs ascertained define radial...
The occurrence and the further spread of high-level glycopeptide-resistant, vanA-positive Enterococcus faecium strains outside hospitals have been investigated. We could isolate such bacteria directly from thawing liquids commercially produced frozen poultry (chickens, turkeys; no data on previous feeding with avoparcin were available). In 5 13 samples raw minced meat pigs originating different butcher's shops, glycopeptide-resistant E. (VanA type) be detected after overnight broth...
ABSTRACT We determined the value of spa typing in combination with BURP (based upon repeat pattern) grouping analysis as a frontline tool epidemiological Staphylococcus aureus , based on random collection 1,459 clinical isolates sent to German Reference Centre for Staphylococci within 6-month period. The application was found be helpful classification into particular clonal lineages currently prevalent Germany. Due its major advantages because ease interpretation and exchangeability results,...
ABSTRACT Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents lactic acid bacteria (LAB) the genera Lactobacillus , Pediococcus Lactococcus and Bifidobacterium . A formulation Iso-Sensitest (90%) deMan-Rogosa-Sharpe (10%) with without supplementation l -cysteine, referred as LAB susceptibility medium, provided most optimal medium basis terms growth support nonenterococcal correct indication international...