A5024901101- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Antifungal resistance and susceptibility
- Escherichia coli research studies
- Milk Quality and Mastitis in Dairy Cows
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Antibiotics Pharmacokinetics and Efficacy
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
- Microbial infections and disease research
- Amino Acid Enzymes and Metabolism
- Orthopedic Infections and Treatments
- Gut microbiota and health
- Cancer Research and Treatments
University of Würzburg
2015-2024
Queen's University Belfast
2007-2010
Friedrich Schiller University Jena
1996-2007
Technische Universität Braunschweig
2007
Institute of Molecular Biotechnology
1999-2005
University of Lübeck
1998
Universität Hamburg
1994
University Medical Center Hamburg-Eppendorf
1994
Biofilm formation of Staphylococcus epidermidis on smooth polymer surfaces has been shown to be mediated by the ica operon. Upon activation this operon, a polysaccharide intercellular adhesin (PIA) is synthesized that supports bacterial cell‐to‐cell contacts and triggers production thick, multilayered biofilms. Thus, gene cluster represents genetic determinant significantly contributes virulence specific strains. PIA synthesis reported recently undergo phase variation process. In study,...
Staphylococcus epidermidis is a common cause of catheter-associated infections and septicemia in immunocompromised patients. To answer the question whether S. skin isolates differ from causing septicemic diseases, 51 strains obtained blood cultures, 1 strain shunt-associated meningitis, 36 saprophytic were characterized. The study demonstrates that most culture formed multilayered biofilm on plastic material, whereas mucosal did not. Moreover, biofilm-producing found to generate large...
Biofilm production is an important step in the pathogenesis of Staphylococcus epidermidis polymer-associated infections and depends on expression icaADBC operon leading to synthesis a polysaccharide intercellular adhesin. A chromosomally encoded reporter gene fusion between ica promoter beta-galactosidase lacZ from Escherichia coli was constructed used investigate influence both environmental factors subinhibitory concentrations different antibiotics S. epidermidis. It shown that biofilm...
Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of global regulator the bacterial response, alternative transcription factor sigma(B), resulted biofilm-negative phenotype and loss salt-induced production. Complementation mutant strain with an expression plasmid encoding sigma(B) completely restored wild-type phenotype. The combined data suggest critical role S. regulation under environmental conditions.
ABSTRACT Concentrations of antibiotics below the MIC are able to modulate expression virulence-associated genes. In this study, influence subinhibitory doses 31 on gene encoding staphylococcal alpha-toxin ( hla ), a major virulence factor Staphylococcus aureus , was investigated with novel fusion protocol. The most striking observation strong induction by concentrations β-lactams and an almost complete inhibition clindamycin. Whereas glycopeptide had no effect, macrolide erythromycin several...
Bacteria are a major cause of infection. To fight disease and growing resistance, research interest is focused on understanding bacterial metabolism. For detailed evaluation the involved mechanisms, precise knowledge molecular composition bacteria required. In this article, various vibrational spectroscopic techniques applied to comprehensively characterize, level, strain Staphylococcus epidermidis, an opportunistic pathogen which has evolved become nosocomial infections. IR absorption...
Staphylococcus epidermidis is a normal constituent of the healthy human microflora, but it also most common cause nosocomial infections associated with use indwelling medical devices. Isolates from device-associated are known for their pronounced phenotypic and genetic variability, in this study we searched factors that might contribute to flexibility. We show mutator phenotypes, which exhibit elevated spontaneous mutation rates, rare among both pathogenic commensal S. strains. However,...
Sequencing of at least 13 Staphylococcus aureus isolates has shown that genomic plasticity impacts significantly on the repertoire virulence factors. However, genome sequencing does not reveal which genes are expressed by individual isolates. Here, we have therefore performed a comprehensive survey composition and variability S. exoproteome. This involved multilocus sequence typing, gene, prophage profiling multiplex PCR, proteomic analyses secreted proteins using 2-DE. Dissection...
ABSTRACT Staphylococcus epidermidis is part of the normal microflora human skin but also a leading cause device-associated infections in critically ill patients. Commensal and clinical S. isolates differ their ability to form biofilms on medical devices; synthesis mediated by icaADBC operon. Currently, epidemiological relatedness between ica- positive -negative not known; neither it known whether ica genes can spread biofilm-negative strains through horizontal gene transfer. In this study,...
Coagulase-negative staphylococci (CoNS) are common opportunistic pathogens, but also ubiquitous human and animal commensals. Infection-associated CoNS from healthcare environments typically characterized by pronounced antimicrobial resistance (AMR) including both methicillin- multidrug-resistant isolates. Less is known about AMR patterns of colonizing the general population. Here we report on in commensal recovered 117 non-hospitalized volunteers a region Germany with high livestock density....
Summary The growing rate of microbial pathogens becoming resistant to standard antibiotics is an important threat public health. In order assess the role in environment on spread resistance factors, impact subinhibitory concentrations sewage gene transfer was investigated using conjugative gentamicin ( aacA–aphD ) plasmids Staphylococcus aureus. Furthermore, concentration hospital measured by high‐performance liquid chromatography (HPLC)–electrospray tandem mass spectrometry. Several were...
Staphylococcus epidermidis and aureus are opportunistic pathogens that cause nosocomial chronic biofilm-associated infections. Indwelling medical devices contact lenses ideal ecological niches for formation of staphylococcal biofilms. Bacteria within biofilms known to display reduced susceptibilities antimicrobials protected from the host immune system. High rates acquired antibiotic resistances in staphylococci other biofilm-forming bacteria further hamper treatment options highlight need...
ABSTRACT Staphylococcus epidermidis and aureus are the most common causes of medical device-associated infections, including septicemic loosenings orthopedic implants. Frequently, microbiological diagnosis these infections remains ambiguous, since at least some staphylococci have capacity to reduce their growth rate considerably. These strains exhibit a small-colony phenotype, often they not detectable by conventional techniques. Moreover, clinical isolates S. adhere polymer metal surfaces...
Abstract In this work we monitor the bacterial growth of a Bacillus pumilus batch culture by means UV resonance Raman spectroscopy. Excitation with wavelength 244 nm especially enhances scattering aromatic amino acids and nucleic acid bases therefore is good method to track metabolic changes that occur during growth. Furthermore, drug from fluoroquinolone group added suspension at beginning exponential phase. With help chemometrical methods such as hierarchical cluster analysis (HCA)...
RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus Bacillus subtilis. Here we show that contributes extracellular (e)DNA release biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production hypervariable epidermidis isolate. Transcriptome analysis through differential sequencing (dRNA-seq) combination with confocal laser scanning...