A5069518872- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Nosocomial Infections in ICU
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Cytomegalovirus and herpesvirus research
- Pediatric health and respiratory diseases
- Antibiotic Use and Resistance
- Parasites and Host Interactions
- Amoebic Infections and Treatments
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Renal Transplantation Outcomes and Treatments
- Pneumonia and Respiratory Infections
- Infective Endocarditis Diagnosis and Management
- Renal Diseases and Glomerulopathies
- Streptococcal Infections and Treatments
- Respiratory viral infections research
- Herpesvirus Infections and Treatments
- Crystallography and molecular interactions
Christophorus Kliniken
2015-2024
Institute of Medical Microbiology and Hygiene
2012-2022
Saarland University
2011-2019
State Laboratory
2018
Leeds Teaching Hospitals NHS Trust
2017
University of Leeds
2017
European Society of Clinical Microbiology and Infectious Diseases
2017
Universitätsklinikum des Saarlandes
2009-2016
University Medical Center
2014
Hospital for Tropical Diseases
2014
Chronic obstructive pulmonary disease (COPD) is a progressive, inflammatory lung that affects large number of patients and has significant impact. One hallmark the presence bacteria in lower airways.The aim this study was to analyze detailed structure microbial communities found lungs healthy individuals with COPD. Nine COPD as compared 9 underwent flexible bronchoscopy BAL performed. Bacterial nucleic acids were subjected terminal restriction fragment (TRF) length polymorphism clone library...
Rates of Clostridium difficile infection vary widely across Europe, as do prevalent ribotypes. The extent Europe-wide diversity within each ribotype, however, is unknown.Inpatient diarrheal fecal samples submitted on a single day in summer and winter (2012-2013) to laboratories 482 European hospitals were cultured for C. difficile, isolates the 10 most ribotypes whole-genome sequenced. Within country-based sequence clustering was assessed using ratio median number single-nucleotide...
Abstract Cytomegalovirus (CMV) is a pathogen of emerging importance for patients with septic shock. In this prospective study, 25 immunocompetent CMV-seropositive shock and an intensive care unit stay >7 days were monitored by using quantitative pp65-antigenemia assay, shell vial culture, virus isolation. Within 2 weeks, active CMV infection low-level (median 3 positive/5 × 105 leukocytes) developed in 8 (32%) patients. Infection was controlled within few weeks 26 days) without use antiviral...
Abstract Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a rare of B-cell origin with frequent expression functional receptors (BCRs). Here we report that cloning followed by antigen screening identifies DNA-directed RNA polymerase beta’ (RpoC) from Moraxella catarrhalis as BCRs IgD + LP cells. Patients show predominance HLA-DRB1*04/07 and the IgVH genes encode extraordinarily long CDR3s. High-titer, light-chain-restricted anti-RpoC IgG1/κ-type serum-antibodies are additionally...
Clostridioides difficile is the primary infectious cause of antibiotic-associated diarrhea. Local transmissions and international outbreaks this pathogen have been previously elucidated by bacterial whole-genome sequencing, but comparative genomic analyses at global scale were hampered lack specific bioinformatic tools. Here we introduce a publicly accessible database within EnteroBase ( http://enterobase.warwick.ac.uk ) that automatically retrieves assembles C. short-reads from public...
Nephropathia epidemica, caused by Puumala virus (PUUV) infection, is a form of hemorrhagic fever with renal syndrome variable severity. Early prognostic markers for the severity failure have not been established. We evaluated clinical and laboratory parameters 15 consecutive patients acute PUUV which endemic in Alb-Danube region, South Germany. Severe (serum creatinine >620 micromol/L) was observed seven patients; four required hemodialysis treatment. Low platelet count (<60 x 109/L), but...
Human cytomegalovirus (CMV) is an important opportunistic pathogen after transplantations. In the present study, monitoring of CMV in patients with septic shock was used to discover whether T helper cell type 1 (Th1) and natural killer (NK) functions interact reactivation not undergoing immunosuppressive therapy.Thirty-eight were monitored, 23 CMV-seropositive included this prospective study.Seven (30.4%) developed active infection despite detection CMV-reactive Th1 cells. After infection,...
ABSTRACT Staphylococcus aureus is a major bacterial pathogen causing variety of diseases ranging from wound infections to severe bacteremia or intoxications. Besides host factors, the course and severity disease also widely dependent on genotype bacterium. Whole-genome sequencing (WGS), followed by bioinformatic sequence analysis, currently most extensive genotyping method available. To identify clinically relevant staphylococcal virulence resistance genes in WGS data, we developed an silico...
A literature survey on the structures and specificities of enzyme inhibitors isolated from microorganisms is presented, with special emphasis protease adrenaline metabolism.
Background The screening of hospital admission patients for methicillin resistant Staphylococcus aureus (MRSA) is undisputed value in controlling and reducing the overall MRSA burden; yet, a concerted parallel universal intervention throughout all hospitals an entire German Federal State has not yet been performed. Methodology/Principal Findings During four-week period, 24 acute care Saarland participated prevalence screening. Overall, 436/20,027 screened revealed carrier status (prevalence,...
Abstract Clonal clusters and gene repertoires of Staphylococcus aureus are essential to understand disease well characterized in industrialized countries but poorly analysed developing regions. The objective this study was compare the molecular-epidemiologic profiles S . isolates from Sub-Saharan Africa Germany. 600 staphylococcal carriers patients with community-associated were by DNA hybridization, clonal complex (CC) attribution, principal component (PCA)-based repertoire analysis. 73%...
Clostridium (Clostridioides) difficile is the main cause for nosocomial diarrhoea in industrialised nations. Epidemiologic data on pathogen's occurrence other world regions are still scarce. In this context we characterized with phenotypic and molecular genetic methods C. isolates stemming from hospitalised patients Lebanon. From 129 stool samples of symptomatic at a tertiary care University hospital Lebanon, total 107 strains were cultivated underwent ribotyping, toxin gene detection...
Clostridioides (Clostridium) difficile infections (CDI) are considered worldwide as emerging health threat. Uptake of C. spores may result in asymptomatic carrier status or lead to CDI that could range from mild diarrhea, eventually developing into pseudomembranous colitis up a toxic megacolon often results high mortality. Most epidemiological studies date have been performed middle- and income countries. Beside others, the use antibiotics composition microbiome identified major risk factors...