A5061378179- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Viral-associated cancers and disorders
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- CNS Lymphoma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Cutaneous lymphoproliferative disorders research
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
- T-cell and B-cell Immunology
- Histiocytic Disorders and Treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- T-cell and Retrovirus Studies
- Immunotherapy and Immune Responses
- Lymphadenopathy Diagnosis and Analysis
- RNA Research and Splicing
- AI in cancer detection
- Cell Image Analysis Techniques
- Sarcoma Diagnosis and Treatment
- Gallbladder and Bile Duct Disorders
- Acute Lymphoblastic Leukemia research
Goethe University Frankfurt
2016-2025
University of Duisburg-Essen
2024-2025
University Hospital Frankfurt
2015-2024
Senckenberg Research Institute and Natural History Museum Frankfurt/M
2013-2023
Goethe Institute
2023
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2022-2023
Klinikum Südstadt Rostock
2015-2022
University of Rostock
1998-2022
University of Tübingen
2021
Universitäts Frauenklinik
2020
Proliferation and survival of Hodgkin Reed/Sternberg (HRS) cells, the malignant cells classical lymphoma (cHL), are dependent on constitutive activation nuclear factor kappaB (NF-kappaB). NF-kappaB through various stimuli is negatively regulated by zinc finger protein A20. To determine whether A20 contributes to pathogenesis cHL, we sequenced TNFAIP3, encoding A20, in HL cell lines laser-microdissected HRS from cHL biopsies. We detected somatic mutations 16 out 36 cHLs (44%), including...
Mantle-cell lymphoma (MCL) is a rather aggressive B-cell malignancy whose considerable variability of individual outcome associated with clinical characteristics (Mantle Cell Lymphoma International Prognostic Index [MIPI]). The Ki-67 index strong independent prognostic factor; however, the biologic MIPI (MIPI-b) distinguishes only two groups, which does not appropriately depict heterogeneity. By using cohort from European MCL Younger and Elderly trials, we aimed to evaluate additional impact...
Purpose To explore the prognostic impact and interdependence of cell-of-origin (COO) classification, dual expression (DE) MYC BCL2 proteins, MYC, BCL2, BCL6 translocations in two prospectively randomized clinical trials patients with diffuse large B-cell lymphoma (DLBCL). Patients Methods Overall, 452 formalin-fixed paraffin-embedded samples from prospective, DLBCL (RICOVER-60, study for > 60 years, all IPI groups; R-MegaCHOEP, ≤ years age-adjusted 2,3) German High-Grade Non-Hodgkin Lymphoma...
Chimeric antigen receptor (CAR) T cells brought substantial benefit to patients with B-cell malignancies. Notwithstanding, CAR T-cell manufacturing requires complex procedures impeding the broad supply chain. Here, we provide evidence that human CD19-CAR can be generated directly
Purpose The differential diagnosis among the commonest peripheral T-cell lymphomas (PTCLs; ie, PTCL not otherwise specified [NOS], angioimmunoblastic lymphoma [AITL], and anaplastic large-cell [ALCL]) is difficult, with morphologic phenotypic features largely overlapping. We performed a phase III diagnostic accuracy study to test ability of gene expression profiles (GEPs; index test) identify subtype. Methods studied 244 PTCLs, including 158 PTCLs NOS, 63 AITLs, 23 ALK-negative ALCLs....
In contrast to the commonly indolent clinical behavior of nodular lymphocyte predominant Hodgkin lymphoma (NLPHL), T cell/histiocyte rich large B cell (THRLBCL) is frequently diagnosed in advanced stages and has a poor prognosis. Besides different presentations these entities, there are variants NLPHL with considerable histopathologic overlap compared THRLBCL. Especially THRLBCL-like NLPHL, diffuse form often presents pattern similar THRLBCL, suggesting close relationship between both...
Cytogenetic analysis of classical Hodgkin's lymphoma is limited by the low content neoplastic Hodgkin-Reed-Sternberg cells in affected tissues. However, available cytogenetic data point to an extreme karyotype complexity. To obtain insights into chromosomal imbalances lymphoma, we applied array-based comparative genomic hybridization (array hybridization) using DNA from microdissected cells.To avoid biases introduced amplification for array hybridization, cHL cases rich were selected....
Significance Multinucleated giant tumor cells are frequently observed in tissue sections of patients with cancer. In classical Hodgkin lymphoma (HL), these so-called Reed–Sternberg (RS) pathognomonic for the disease. Despite well-described disease-promoting functions multinucleated RS cells, their development remains obscure. Long-term time-lapse microscopy and single-cell tracking HL cell lines demonstrated that generated by re-fusion small mononuclear progenitors. Importantly, fusing share...
Summary Nodular lymphocyte predominant Hodgkin lymphoma ( NLPHL ) and T cell/histiocyte rich large B cell THRLBCL usually affect middle‐aged men, show tumour cells with a phenotype low content. Whereas the clinical behaviour of is indolent, presents advanced stage disease an aggressive behaviour. In present study, array comparative genomic hybridization was performed in seven typical , four ‐like variants, six diffuse lymphomas DLBCL derived from . The number aberrations higher compared...
To investigate the expression of programmed cell death 1 (PD-1) receptor-programmed ligand (PD-L1) pathway and clinicopathological characteristics in extrahepatic cholangiocarcinoma (eCCA).Tissue samples from patients with eCCA [n = 69; perihilar (CCA), 40; distal CCA, 29] who underwent surgical resection period 2007 to 2015 were evaluated for PD-1, PD-L1, CD3 CD163 expression, correlations characteristics, including survival data, determined. PD-L1 was found on both tumour cells (8/69,...
Angioimmunoblastic T-cell lymphomas (AITLs) frequently carry mutations in the TET2 and IDH2 genes. represent early genetic lesions as they had already been detected haematopoietic precursor cells of AITL patients. We show by analysis whole-tissue sections microdissected PD1+ that frequency TET2-mutated is presumably even higher than reported (12/13 cases our collection; 92%). In two-thirds informative AITLs (6/9), a fraction B was also TET2-mutated. Investigation four IGHV gene sequencing...
Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a rare cancer, and large international cooperative efforts are needed to evaluate the significance of clinical risk factors immunoarchitectural patterns (IAPs) for all stages pediatric adult patients with NLPHL.
T-cell/histiocyte-rich large B-cell lymphoma is a rare aggressive showing histopathological overlap with nodular lymphocyte-predominant Hodgkin lymphoma. Despite differences in tumor microenvironment and clinical behavior, the cells of both entities show remarkable similarities, suggesting that lymphomas might represent spectrum same disease. To address this issue, we investigated whether these share mutations. Ultra-deep targeted resequencing six typical 11 variants lymphoma, nine cases...
Follicular dendritic cell (FDC)-sarcoma is a rare neoplasm with morphologic and phenotypic features of FDCs. It shows an extremely heterogeneous morphology, therefore, its diagnosis relys on the phenotype tumor cells. Aim present study was identification new specific markers for FDC-sarcoma by whole transcriptome sequencing (WTS). Candidate were selected based gene expression level biological function. Immunohistochemistry performed reactive tonsils, 22 cases FDC-sarcomas 214 control...