A5075213468- Cancer, Hypoxia, and Metabolism
- Liver Disease Diagnosis and Treatment
- Protein Kinase Regulation and GTPase Signaling
- MicroRNA in disease regulation
- Hepatitis C virus research
- Hepatocellular Carcinoma Treatment and Prognosis
- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Receptor Mechanisms and Signaling
- Circular RNAs in diseases
- Neuropeptides and Animal Physiology
- RNA modifications and cancer
- Pancreatitis Pathology and Treatment
- Peptidase Inhibition and Analysis
- Nanoparticle-Based Drug Delivery
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- RNA Interference and Gene Delivery
- Diabetes Treatment and Management
- Liver physiology and pathology
- Nanoplatforms for cancer theranostics
- Hepatitis B Virus Studies
- Cancer Research and Treatments
- Cellular transport and secretion
- Cancer-related molecular mechanisms research
Goethe University Frankfurt
2014-2024
University Hospital Frankfurt
2014-2024
German Cancer Research Center
2022-2023
Heidelberg University
2022-2023
Goethe Institute
2012-2013
Universität Hamburg
2012
Nanosystem (Russia)
2012
University Medical Center Hamburg-Eppendorf
2012
Research Institute of Human Morphology
2012
University of Freiburg
2011
The liver contains large amounts of microRNA-122 (miR-122), whereas other tissues contain only marginal this miRNA. MicroRNAs have also been found to circulate in the blood a cell-free form; their potential as readily accessible disease markers is currently evaluated. Here, we investigated if serum levels miR-122 might be useful parameter patients with chronic hepatitis C virus (HCV) infection.RNA was extracted from sera HCV infection (CHC) and healthy controls analyzed for miR-22 content by...
Background MicroRNAs circulating in the blood, stabilized by complexation with proteins and/or additionally encapsulation lipid vesicles, are currently being evaluated as biomarkers. The consequences of their differential association lipids/vesicles for stability and use biomarkers largely unexplored subject present study. Methods levels a set selected microRNAs were determined quantitative reverse-transcription PCR after extraction from sera or vesicle- non-vesicle fractions prepared sera....
Background MicroRNA-21 (miR-21) is up-regulated in tumor tissue of patients with malignant diseases, including hepatocellular carcinoma (HCC). Elevated concentrations miR-21 have also been found sera or plasma from malignancies, rendering it an interesting candidate as serum/plasma marker for malignancies. Here we correlated serum levels clinical parameters different stages chronic hepatitis C virus infection (CHC) and CHC-associated HCC. Methodology/Principal Findings 62 CHC patients, 29...
Summary. miR‐122 is a liver‐specific microRNA, which also circulates in the blood. The levels of serum and plasma correlate with hepatic necroinflammation patients hepatitis B virus (HBV) infection. Here, we investigated whether surrogate markers for viral replication translation. Furthermore, examined differ different groups HBV‐infected may be useful to identify higher or lower risk liver disease progression. Therefore, RNA was extracted from sera therapy‐naïve HBV infection ( n = 89)...
Summary Background Proton pump inhibitors ( PPI ) are widely used in patients with liver diseases. Within the last years, there have been concerns about use as they may promote infections cirrhosis. Aim As sparse data of prognostic relevance treatment, to perform a prospective study investigating relation treatment and overall survival OS cirrhotic individuals. Methods Patients cirrhosis were enrolled followed prospectively. The primary end point was . additional clinical laboratory assessed...
Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor, usually arises in setting of cirrhosis (LC), and has a poor prognosis. The recently discovered Th2-cytokine interleukin-33 (IL-33) possible mediator pancreatic gastric carcinogeneses. IL-33 binds to its receptor soluble ST2 (sST2), which thereby acts as regulator. role sST2 HCC not been elucidated yet.We conducted case-control study with 130 patients 50 healthy controls (HCs). Sixty-five suffered from 65 had LC...
Aim Cellular CD81 is a well characterized hepatitis C virus (HCV) entry factor, while the relevance of soluble exosomal in HCV pathogenesis poorly defined. We performed case-control study to investigate whether serum fraction associated with replication and inflammatory activity. Patients Methods Four cohorts were investigated, patients chronic (n = 37), infection persistently normal ALT levels 24), long term sustained virologic response (SVR, n 7), healthy volunteers 23). Concentration was...
Summary Background Vitamin D is involved in many biological processes. The role of vitamin patients with hepatocellular carcinoma ( HCC ) remains inconclusive, although there evolving evidence that may modulate cancer development and progression. Aim To evaluate serum as prognostic parameter , we performed a prospective cohort study. Methods were prospectively recruited 25‐hydroxyvitamin D3 (25( OH )D 3 levels determined. 25( compared to stages cirrhosis nonparametric Kruskal–Wallis tests...
// Georgios Grammatikos 1, 2 , Niklas Schoell Nerea Ferreirós 3 Dimitra Bon 4 Eva Herrmann Harald Farnik Verena Köberle Albrecht Piiper Stefan Zeuzem Bernd Kronenberger Oliver Waidmann 2, * Josef Pfeilschifter 1 Goethe University Hospital, Pharmazentrum Frankfurt, Institut für Allgemeine Pharmakologie und Toxikologie, Frankfurt am Main, Germany Medizinische Klinik klinische Pharmakologie, University, Department of Medicine, Institute Biostatistics and Mathematical Modelling,...
Background Liver cirrhosis is associated with high morbidity and mortality. MicroRNAs (miRs) circulating in the blood are an emerging new class of biomarkers. In particular, serum level liver-specific miR-122 might be a clinically useful parameter patients acute or chronic liver disease. Aim Here we investigated if prognostic cirrhosis. Methods 107 test cohort 143 validation were prospectively enrolled into present study. RNA was extracted from sera obtained at time study enrollment...
Epithelial‐mesenchymal transition ( EMT ) is an important mechanism to initiate cancer invasion and metastasis. Bone morphogenetic protein BMP )‐9 a member of the transforming growth factor TGF )‐β superfamily. It has been suggested play role in development some non‐hepatic tumors. In present study, two hepatocellular carcinoma HCC lines, HLE H ep G 2, were treated with ‐9 vitro , phenotypic changes cell motility analyzed. situ hybridization ISH immunohistochemical analyses performed human...
Background & Aims Vitamin D, best known to regulate bone mineralization, has numerous additional roles including regulation inflammatory pathways. Recently, an increased incidence of 25-hydroxyvitamin D3 (25(OH)D3) deficiency been found in subjects suffering from liver diseases. We here investigated if low vitamin D levels might be associated with prognosis, inflammation and infectious complications patients cirrhosis. Methods performed a prospective cohort study investigating the relation...
Elevation of the intracellular cAMP concentration ([cAMP]i) regulates metabolism, cell proliferation, and differentiation plays roles in memory formation neoplastic growth. mediates its effects mainly through activation protein kinase A (PKA) as well Epac1 Epac2, exchange factors activating small GTPases Rap1 Rap2. However, how utilizes these effectors to induce distinct biological responses is unknown. We here studied specific PKA Epac neuroendocrine PC12 cells. In cells, elevation [cAMP]i...
Background Autotaxin (ATX) and its product lysophosphatidic acid (LPA) are considered to be involved in the development of liver fibrosis elevated levels serum ATX have been found patients with hepatitis C virus associated fibrosis. However, clinical role systemic stages cirrhosis was unknown. Here we investigated relation severity as well prognosis cirrhotic patients. Methods Patients were prospectively enrolled followed until death, transplantation or last contact. Blood samples drawn at...
Abstract iRGD is a derivative of the integrin-binding peptide RGD, which selectively increases penetrability tumor tissue to various coadministered substances in several preclinical models. In this study, we investigated ability improve delivery sorafenib and doxorubicin therapy hepatocellular carcinoma (HCC) using established mouse models disease. A contrast-enhanced MRI method was developed parallel assess vivo effects setting. We found that improved marker tumors HCC-bearing mice about...
TGF-β1 is a major player in chronic liver diseases promoting fibrogenesis and tumorigenesis through various mechanisms. The expression function of TGF-β2 have not been investigated thoroughly disease to date. In this paper, we provide evidence that correlates with cancer development.Using quantitative realtime PCR ELISA, show mRNA secretion increased murine HSCs hepatocytes over time culture were found the human-derived HSC cell line LX-2. stimulation LX-2 cells led upregulation TGF-β...