A5073981529- Hepatitis C virus research
- MicroRNA in disease regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gallbladder and Bile Duct Disorders
- Circular RNAs in diseases
- Pediatric Hepatobiliary Diseases and Treatments
- Lymphoma Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Chronic Lymphocytic Leukemia Research
- Cancer Genomics and Diagnostics
- Liver Disease Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Hepatocellular Carcinoma Treatment and Prognosis
- Gastric Cancer Management and Outcomes
- Extracellular vesicles in disease
- Viral-associated cancers and disorders
- Neuroendocrine Tumor Research Advances
- Hepatitis B Virus Studies
- Lung Cancer Research Studies
- Clinical Nutrition and Gastroenterology
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Metastasis and carcinoma case studies
- PI3K/AKT/mTOR signaling in cancer
Klinikum Stuttgart
2023-2025
Robert Bosch Hospital
2017-2023
Goethe University Frankfurt
2011-2020
University Hospital Frankfurt
2011-2020
University Medical Center Freiburg
2011
University of Freiburg
2011
Inserm
2011
Université Claude Bernard Lyon 1
2011
The liver contains large amounts of microRNA-122 (miR-122), whereas other tissues contain only marginal this miRNA. MicroRNAs have also been found to circulate in the blood a cell-free form; their potential as readily accessible disease markers is currently evaluated. Here, we investigated if serum levels miR-122 might be useful parameter patients with chronic hepatitis C virus (HCV) infection.RNA was extracted from sera HCV infection (CHC) and healthy controls analyzed for miR-22 content by...
Background MicroRNAs circulating in the blood, stabilized by complexation with proteins and/or additionally encapsulation lipid vesicles, are currently being evaluated as biomarkers. The consequences of their differential association lipids/vesicles for stability and use biomarkers largely unexplored subject present study. Methods levels a set selected microRNAs were determined quantitative reverse-transcription PCR after extraction from sera or vesicle- non-vesicle fractions prepared sera....
Background MicroRNA-21 (miR-21) is up-regulated in tumor tissue of patients with malignant diseases, including hepatocellular carcinoma (HCC). Elevated concentrations miR-21 have also been found sera or plasma from malignancies, rendering it an interesting candidate as serum/plasma marker for malignancies. Here we correlated serum levels clinical parameters different stages chronic hepatitis C virus infection (CHC) and CHC-associated HCC. Methodology/Principal Findings 62 CHC patients, 29...
To investigate the expression of programmed cell death 1 (PD-1) receptor-programmed ligand (PD-L1) pathway and clinicopathological characteristics in extrahepatic cholangiocarcinoma (eCCA).Tissue samples from patients with eCCA [n = 69; perihilar (CCA), 40; distal CCA, 29] who underwent surgical resection period 2007 to 2015 were evaluated for PD-1, PD-L1, CD3 CD163 expression, correlations characteristics, including survival data, determined. PD-L1 was found on both tumour cells (8/69,...
Summary Many epidemiological studies provide solid evidence for an association of chronic hepatitis C virus ( HCV ) infection with B‐cell non‐Hodgkin's lymphoma (B‐ NHL ). However, the most convincing a causal relationship between and development is observation B‐ regression after eradication by antiviral therapy AVT We conducted literature search to identify that included patients ‐associated ‐ who received , intention treat viral disease at same time. The primary end point was correlation...
Immune-modulating therapy is a promising for patients with cholangiocarcinoma (CCA). Microsatellite instability (MSI) might be favorable predictor treatment response, but comprehensive data on the prevalence of MSI in CCA are missing. The aim current study was to determine German tertiary care hospital. Formalin-fixed paraffin-embedded tissue samples, obtained period from 2007 2015 undergoing surgical resection curative intention at Johann Wolfgang Goethe University hospital, were examined....
Data on intratumoral heterogeneity of small intestine neuroendocrine tumors (SI-NETs) and related liver metastasis are limited. The aim this study was to characterize genetic 5 patients with SI-NETs. Therefore, formalin-fixed, paraffin-embedded tissue samples primary metastatic lesions as well benign five synchronously metastasized, differentiated SI-NETs were analyzed whole exome sequencing. For one patient, chip based 850k DNA methylome analysis performed tumor control tissue. Thereby, 156...
// Dirk Walter 1, 2 , Claudia Döring Magdalena Feldhahn 3 Florian Battke Sylvia Hartmann Ria Winkelmann Markus Schneider Katrin Bankov 1 Andreas Schnitzbauer 4 Stefan Zeuzem Martin Leo Hansmann Jan Peveling-Oberhag 2, 5 Department of Internal Medicine I, Johann Wolfgang Goethe-University Hospital, 60590 Frankfurt, Germany Dr. Senckenberg Institute Pathology, CeGaT GmbH, 72076 Tuebingen, General and Visceral Surgery, for Gastroenterology, Hepatology Endocrinology, Robert-Bosch 70376...
Epigenetic silencing of tumour suppressor genes has been observed in various cancers. Looking at hepatocellular carcinoma (HCC) specific protein was previously demonstrated to be associated with the Hepatitis C virus (HCV). However, proposed HCV dependent promoter methylation DNA mismatch repair (MMR) and thereby enhanced progression hepatocarcinogenesis subject controversial discussion. We investigated pattern MMR MLH1, MSH2 PMS2 as well cyclin-dependent kinase inhibitor 2A gene (p16) 61...
Splenic marginal zone lymphoma (SMZL) is an indolent B-cell non-Hodgkin and represents the most common primary malignancy of spleen. Its precise molecular pathogenesis still unknown specific markers for diagnosis or possible targets causal therapies are lacking.We performed whole exome sequencing (WES) copy number analysis from laser-microdissected tumor cells two SMZL discovery cases. Selected somatic single nucleotide variants (SNVs) were analyzed using pyrosequencing Sanger in independent...
Abstract Background and Aim A number of studies were able to show a reduction hypoxemia episodes during procedural sedation through the use capnography (CA). The present study investigates percutaneous endoscopic gastrostomy (PEG) placement with propofol comparing standard monitoring (SM) versus SM additional CA surveillance. Methods In this single center randomized controlled trial, 150 patients prospectively 1:1 in either group or after stratification for ASA class, PEG method (push pull...
Hepatitis C virus (HCV) infection leads to the development of hepatic diseases, as well extrahepatic disorders such B-cell non-Hodgkin's lymphoma (B-NHL). To reveal molecular signalling pathways responsible for HCV-associated B-NHL development, we utilised transgenic (Tg) mice that express full-length HCV genome specifically in B cells and develop non-Hodgkin type lymphomas (BCLs). The gene expression profiles from BCL-developing HCV-Tg mice, BCL-non-developing HCV-negative were analysed by...
Standard monitoring of heart rate, blood pressure and arterial oxygen saturation during endoscopy is recommended by current guidelines on procedural sedation. A number studies indicated a reduction hypoxic (art. oxygenation < 90% for > 15 s) severe events 85%) additional use capnography. Therefore, U.S. the European comment that capnography can be considered in long or deep Integrated Pulmonary Index® (IPI) an algorithm-based parameter combines measured pulse oximetry oxygenation, rate)...
Summary We characterized the early dynamics of hepatitis B virus ( HBV ) quasispecies evolution during first weeks antiviral therapy with low‐to‐moderate genetic barrier drugs and associated these data response patterns. Fifteen chronic patients (men, 10; mean age, 34; HB e A g positive, 6) who received lamivudine or telbivudine for at least 52 were included. DNA was extracted from serum, a 910‐bp fragment covering domains A–F reverse transcriptase region amplified, cloned sequenced....