Stefan Alig
A5068749819- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Genetic factors in colorectal cancer
- CNS Lymphoma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Viral-associated cancers and disorders
- Molecular Biology Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Glioma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- Acute Myeloid Leukemia Research
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Ovarian cancer diagnosis and treatment
- Mycobacterium research and diagnosis
- Hematopoietic Stem Cell Transplantation
- Brain Metastases and Treatment
- Pancreatic and Hepatic Oncology Research
Stanford University
2019-2025
Ludwig-Maximilians-Universität München
2013-2025
Essen University Hospital
2025
Stanford Medicine
2021-2022
Munich Leukemia Laboratory (Germany)
2018-2021
University Hospital Cologne
2021
Mayo Clinic in Arizona
2021
Cancer Genetics (United States)
2021
Columbia University
2021
Herbert Irving Comprehensive Cancer Center
2021
PURPOSE Clinical outcomes of patients with CNS lymphomas (CNSLs) are remarkably heterogeneous, yet identification at high risk for treatment failure is challenging. Furthermore, CNSL diagnosis often remains unconfirmed because contraindications invasive stereotactic biopsies. Therefore, improved biomarkers needed to better stratify into groups, predict response, and noninvasively identify CNSL. PATIENTS AND METHODS We explored the value circulating tumor DNA (ctDNA) early outcome prediction,...
CREBBP mutations are highly recurrent in B-cell lymphomas and either inactivate its histone acetyltransferase (HAT) domain or truncate the protein. Herein, we show that these two classes of yield different degrees disruption epigenome, with HAT being more severe associated inferior clinical outcome. Genes perturbed by mutation direct targets BCL6-HDAC3 onco-repressor complex. Accordingly, HDAC3-selective inhibitors reverse CREBBP-mutant aberrant epigenetic programming, resulting in: (i)...
Patients with Diffuse Large B-cell Lymphoma (DLBCL) in need of immediate therapy are largely under-represented clinical trials. The diagnosis-to-treatment interval (DTI) has recently been described as a metric to quantify such patient selection bias, short DTI being associated adverse risk factors and inferior outcomes. Here, we characterized the relationships between DTI, circulating tumor DNA (ctDNA), conventional factors, outcomes, goal defining objective disease metrics contributing...
Tumor cells orchestrate their microenvironment. Here, we provide biochemical, structural, functional, and clinical evidence that Cathepsin S (CTSS) alterations induce a tumor-promoting immune microenvironment in follicular lymphoma (FL). We found CTSS mutations at Y132 6% of FL (19/305). Another 13% (37/286) had amplification, which was associated with higher expression. lead to accelerated autocatalytic conversion from an enzymatically inactive profrom active increased substrate cleavage,...
The tissue architecture of classic Hodgkin Lymphoma (CHL) is ｕｎｉｑｕｅ among cancers and characterized by rare malignant Reed-Sternberg cells that co-evolve with a complex ecosystem immune in the tumor microenvironment (TME). lack comprehensive systems-level interrogation has hindered description disease heterogeneity clinically relevant molecular subtypes. Here, we employed an integrative, multimodal approach to characterize CHL tumors using cell sequencing, spatial transcriptomics imaging...
The vascular endothelial growth factor (VEGF) stimulates angiogenesis by induction of vessel permeability, proliferation, and migration cells, an important process in ischaemic diseases. ADP-ribosylation (ARF) nucleotide-binding site opener (ARNO) (cytohesin-2) is a guanine exchange for cellular signalling through ARF GTPases. However, role ARNO VEGF-dependent processes has so far not been documented. Therefore, we investigated whether activation cells thus permeability. expression was...
The Follicular Lymphoma (FL) International Prognostic Index (FLIPI) and FLIPI-2 are well-described clinical risk models. Age >60 years at diagnosis is a factor in both scores. Recently, we showed that older age not associated with higher of disease progression or inferior treatment efficacy. Instead, shorter survival patients results mainly from an increased nonrelapse deaths. This questions the value as meaningful component scores intended to predict disease-specific survival. newly...
Introduction The tyrosine phosphatase SHP-1 negatively influences endothelial function, such as VEGF signaling and reactive oxygen species (ROS) formation, has been shown to influence angiogenesis during tissue ischemia. In ischemic tissues, hypoxia induced is crucial for restoring supply. However, the exact mechanism how affects function ischemia or remains unclear. We performed in vitro cell culture experiments characterize role of hypoxia. Results knock-down by specific antisense...
Abstract Diffuse large B-cell lymphoma (DLBCL) can be cured with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP); however, one-third of patients experience refractory or relapsed disease. Studies comparing R-CHOP modified regimens replacing R obinutuzumab (O) adding lenalidomide (L) did not result in improved outcomes; L O together may enhance natural killer-cell mediated antibody-dependent cellular toxicity when paired CHOP. Here, we report on a phase 1b/2...
This pilot study aimed on generating insight alterations in circulating immune cells during the use of FOLFIRINOX and gemcitabine/nab-paclitaxel pancreatic ductal adenocarcinoma (PDAC). Peripheral blood mononuclear were isolated before 30 days after initiation chemotherapy from 20 patients with advanced PDAC. Regulatory T (FoxP3+) checkpoints (PD-1 TIM-3) analyzed by flow cytometry immunological changes correlated clinical outcome. Heterogeneous observed T-cell subpopulations a pronounced...
Introduction . Inflammation and endothelium-derived superoxides are important pathomechanisms in atherothrombotic diseases. We could previously show that the tyrosine phosphatase SHP-1 acts as a negative regulator endothelial superoxide production. In this study we investigated influence of on platelet-endothelium interaction arterial thrombosis TNF α -induced inflammation vivo Methods Arteriolar platelet rolling were C57BL/6 mice using intravital microscopy dorsal skinfold chamber...