David G. Huntsman
A5085086748- Ovarian cancer diagnosis and treatment
- Cancer Genomics and Diagnostics
- Endometrial and Cervical Cancer Treatments
- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Genetic factors in colorectal cancer
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Endometriosis Research and Treatment
- HER2/EGFR in Cancer Research
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- BRCA gene mutations in cancer
- Virus-based gene therapy research
- Reproductive Biology and Fertility
- Sarcoma Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Protein Degradation and Inhibitors
- Breast Cancer Treatment Studies
- Molecular Biology Techniques and Applications
- Kruppel-like factors research
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
University of British Columbia
2016-2025
BC Cancer Agency
2015-2024
Vancouver General Hospital
2015-2024
Molecular Oncology (United States)
2017-2024
Spinal Cord Injury BC
2020-2024
Occupational Cancer Research Centre
2011-2024
Cancer Research Center
2024
Genome British Columbia
2020-2024
The Prostate Centre
2009-2023
Hereditary Disease Foundation
2023
Ovarian clear-cell and endometrioid carcinomas may arise from endometriosis, but the molecular events involved in this transformation have not been described.We sequenced whole transcriptomes of 18 ovarian 1 carcinoma cell line found somatic mutations ARID1A (the AT-rich interactive domain 1A [SWI-like] gene) 6 samples. encodes BAF250a, a key component SWI–SNF chromatin remodeling complex. We an additional 210 second measured BAF250a expression by means immunohistochemical analysis 455...
Next-generation sequencing approaches have been used to investigate the genomes and transcriptomes of an oestrogen-receptor-α-positive metastatic lobular breast cancer from a patient — rather than cell line or xenograft over 9-year period between diagnosis primary tumour appearance metastasis. Comparison somatic non-synonymous coding mutations in metastasis same combined analysis genome transcriptome data provided insights into mutational evolution that can occur with disease progression....
Endometriosis is a risk factor for epithelial ovarian cancer; however, whether this extends to all invasive histological subtypes or borderline tumours not clear. We undertook an international collaborative study assess the association between endometriosis and of cancer.
Although it has long been appreciated that ovarian carcinoma subtypes (serous, clear cell, endometrioid, and mucinous) are associated with different natural histories, most biomarker studies current treatment protocols for women this disease not subtype specific. With the emergence of high-throughput molecular techniques, distinct pathogenetic pathways have identified in these subtypes. We examined variation expression rates between subtypes, how influences correlations stage at diagnosis or...
Classification of endometrial carcinomas (ECs) by morphologic features is inconsistent, and yields limited prognostic predictive information. A new system for classification based on the molecular categories identified in The Cancer Genome Atlas proposed. Genomic data from (TCGA) support into four prognostically significant subgroups; we used TCGA set to develop surrogate assays that could replicate classification, but without need labor-intensive cost-prohibitive genomic methodology....
BACKGROUND Classification of endometrial carcinomas (ECs) by morphologic features is irreproducible and imperfectly reflects tumor biology. The authors developed the Proactive Molecular Risk Classifier for Endometrial Cancer (ProMisE), a molecular classification system based on Genome Atlas genomic subgroups, sought to confirm both feasibility prognostic ability in new, large cohort ECs. METHODS Immunohistochemistry (IHC) presence or absence mismatch repair (MMR) proteins (to identify MMR...
Granulosa-cell tumors (GCTs) are the most common type of malignant ovarian sex cord-stromal tumor (SCST). The pathogenesis these is unknown. Moreover, their histopathological diagnosis can be challenging, and there no curative treatment beyond surgery.We analyzed four adult-type GCTs using whole-transcriptome paired-end RNA sequencing. We identified putative GCT-specific mutations that were present in at least three samples but absent from transcriptomes 11 epithelial tumors, published human...
<h3>Importance</h3> E-cadherin (<i>CDH1</i>) is a cancer predisposition gene mutated in families meeting clinically defined hereditary diffuse gastric (HDGC). Reliable estimates of risk and spectrum germline mutation carriers are essential for management. For without<i>CDH1</i>mutations, genetic-based stratification has not been possible, resulting limited clinical options. <h3>Objectives</h3> To derive accurate breast risks in<i>CDH1</i>mutation determine if mutations other genes associated...
Because of the high risk recurrence in high-grade serous ovarian carcinoma (HGS-OvCa), development outcome predictors could be valuable for patient stratification. Using catalog The Cancer Genome Atlas (TCGA), we developed subtype and survival gene expression signatures, which, when combined, provide a prognostic model HGS-OvCa classification, named "Classification Ovarian Cancer" (CLOVAR). We validated CLOVAR on an independent dataset consisting 879 profiles. worst group, accounting 23% all...
Gene fusions created by somatic genomic rearrangements are known to play an important role in the onset and development of some cancers, such as lymphomas sarcomas. RNA-Seq (whole transcriptome shotgun sequencing) is proving be a useful tool for discovery novel gene cancer transcriptomes. However, algorithmic methods using data remain underdeveloped. We have developed deFuse, computational method fusion tumor data. Unlike existing that use only unique best-hit alignments consider boundaries...
25–30% of families fulfilling the criteria for hereditary diffuse gastric cancer have germline mutations <i>CDH1</i> (E-cadherin) gene. In light new data and advancement technologies, a multidisciplinary workshop was convened to discuss genetic testing, surgery, endoscopy pathology reporting. The updated recommendations include broadening testing such that: histological confirmation is only required one family member; inclusion individuals with before age 40 years without history; diagnoses...
Endometriosis, defined as the presence of ectopic endometrial stroma and epithelium, affects approximately 10% reproductive-age women can cause pelvic pain infertility. Endometriotic lesions are considered to be benign inflammatory but have cancerlike features such local invasion resistance apoptosis.
Tenosynovial giant-cell tumor (TGCT) and pigmented villonodular synovitis (PVNS) are related conditions with features of both reactive inflammatory disorders clonal neoplastic proliferations. Chromosomal translocations involving chromosome 1p13 have been reported in TGCT PVNS. We confirm that present a majority cases PVNS show CSF1 is the gene at breakpoint. In some PVNS, fused to COL6A3 (2q35). The result overexpression CSF1. carrying this translocation, it minority intratumoral cells,...
Hereditary diffuse gastric cancer is caused by germline mutations in the epithelial cadherin (CDH1) gene and characterized an increased risk for lobular breast cancer.To determine whether recurring CDH1 occurred due to independent mutational events or common ancestry.Thirty-eight families diagnosed clinically with hereditary were accrued between November 2004 January 2006 analyzed as part of ongoing study at British Columbia Cancer Agency. Twenty-six had least 2 cases 1 case a person younger...