Ie‐Ming Shih
A5058026421- Ovarian cancer diagnosis and treatment
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Viral-associated cancers and disorders
- Chromatin Remodeling and Cancer
- PARP inhibition in cancer therapy
- Cancer Genomics and Diagnostics
- Mechanisms of cancer metastasis
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
- Gene expression and cancer classification
- Endometrial and Cervical Cancer Treatments
- Cancer Mechanisms and Therapy
- RNA modifications and cancer
- Virus-based gene therapy research
- Genetic and Kidney Cyst Diseases
- Gestational Trophoblastic Disease Studies
- Cancer, Lipids, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Reproductive Biology and Fertility
- Animal Virus Infections Studies
- Kruppel-like factors research
- Cancer Cells and Metastasis
- Genomic variations and chromosomal abnormalities
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2015-2025
Sidney Kimmel Comprehensive Cancer Center
2002-2024
University of Baltimore
2010-2024
University of Ulsan
2023
Ulsan College
2023
Asan Medical Center
2023
Beijing Obstetrics and Gynecology Hospital
2023
Johns Hopkins Hospital
2017-2022
Bloomberg (United States)
2022
Activating mutations in KRAS and one of its downstream mediators, BRAF, have been identified a variety human cancers. To determine the role BRAF ovarian carcinoma, we analyzed both genes for three common (at codon 599 codons 12 13 KRAS). Mutations either or occurred 15 22 (68%) invasive micropapillary serous carcinomas (MPSCs; low-grade tumors) 31 51 (61%) borderline tumors (precursor lesions to MPSCs). None contained mutation KRAS. In contrast, none 72 conventional aggressive high-grade two...
Chromosomal instability (CIN) is a defining characteristic of most human cancers. Mutation CIN genes increases the probability that whole chromosomes or large fractions are gained lost during cell division. The consequence an imbalance in number per (aneuploidy) and enhanced rate loss heterozygosity. A major question cancer genetics to what extent CIN, any genetic instability, early event consequently driving force for tumor progression. In this article, we develop mathematical framework...
Endometriosis, defined as the presence of ectopic endometrial stroma and epithelium, affects approximately 10% reproductive-age women can cause pelvic pain infertility. Endometriotic lesions are considered to be benign inflammatory but have cancerlike features such local invasion resistance apoptosis.
The integration of mass spectrometry-based proteomics with next-generation DNA and RNA sequencing profiles tumors more comprehensively. Here this "proteogenomics" approach was applied to 122 treatment-naive primary breast cancers accrued preserve post-translational modifications, including protein phosphorylation acetylation. Proteogenomics challenged standard cancer diagnoses, provided detailed analysis the ERBB2 amplicon, defined tumor subsets that could benefit from immune checkpoint...
Abstract Serous tubal intraepithelial carcinomas (STICs) have been proposed to be the most likely precursor of ovarian, and ‘primary peritoneal’ (pelvic) high‐grade serous carcinoma (HGSC). As somatic mutation TP53 is common molecular genetic change ovarian HGSC, occurring in more than 95% cases, we undertook a mutational analysis 29 pelvic HGSCs that had concurrent STICs demonstrate clonal relationship HGSCs. In addition, correlated data with p53 immunostaining determine role...
BackgroundMicroRNAs (miRNAs) represent a class of small non-coding RNAs that control gene expression by targeting mRNAs and triggering either translation repression or RNA degradation. Emerging evidence suggests the potential involvement altered regulation miRNA in pathogenesis cancers, these genes are thought to function as both tumor suppressors oncogenes.Methodology/Principal FindingsUsing microRNA microarrays, we identify several miRNAs aberrantly expressed human ovarian cancer tissues...
Abstract Context.—Current tumor markers for ovarian cancer still lack adequate sensitivity and specificity to be applicable in large populations. High-throughput proteomic profiling bioinformatics tools allow the rapid screening of a number potential biomarkers serum, plasma, or other body fluids. Objective.—To determine whether protein profiles plasma can used identify that improve detection cancer. Design.—We analyzed samples had been collected between 1998 2001 from patients with sporadic...
It has been well established that ovarian low-grade and high-grade serous carcinomas are fundamentally different types of tumours. While the molecular genetic features now known, pathogenesis carcinomas, apart from recognition frequent somatic mutations involving KRAS BRAF, is largely unknown. In order to comprehensively analyse in we applied exome sequencing DNA eight samples affinity-purified, low-grade, carcinomas. A remarkably small number were identified seven these tumours: a total 70...
Recent evidence suggests that ovarian high-grade serous carcinoma (HGSC) originates from the epithelium of fallopian tube. However, most mouse models are based on previous prevailing view cancer develops transformation surface epithelium. Here, we report extensive histological and molecular characterization mogp-TAg transgenic mouse, which expresses SV40 large T-antigen (TAg) under control müllerian-specific Ovgp-1 promoter. Histological analysis tubes mice identified a variety neoplastic...
The clonal relationship between ovarian high-grade serous carcinoma (HGSC) and its presumed precursor lesion, tubal intraepithelial (STIC), has been reported. However, when analyzing patients with concurrent the extensive tissues present at diagnosis may have effaced natural habitat of clone(s), obscuring tumor evolutionary history, or disseminated to anatomically adjacent fimbriae ends, masquerading as lesions. To circumvent these limitations, we analyzed genomic landscape incidental...
Many gene products exhibit great structural heterogeneity because of an array modifications. These modifications are not directly encoded in the genomic template but often affect functionality proteins. Protein glycosylation plays a vital role proper protein functions. However, analysis glycoproteins has been challenging compared with other modifications, such as phosphorylation. Here, we perform integrated proteomic and glycoproteomic 83 prospectively collected high-grade serous ovarian...
ObjectivePlatinum-resistant, high-grade serous ovarian cancer (HGSOC) has limited treatment options. Preclinical data suggest that poly(ADP-ribose) polymerase inhibitors (PARPi) and ataxia telangiectasia Rad3-related kinase (ATRi) are synergistic. CAPRI (NCT03462342) is an investigator-initiated study of olaparib plus ceralasertib in recurrent HGSOC. Herein, we present results from the platinum-resistant cohort.MethodsA Simon 2-stage design was utilized. Platinum-resistant HGSOC patients...
Compared with high-grade serous carcinoma, low-grade carcinoma of the ovary or peritoneum is a less frequent epithelial ovarian cancer type that poorly sensitive to chemotherapy and affects younger women, many whom endure years ineffective treatments poor quality life. The pathogenesis this disease its management remain incompletely understood. However, recent advances in molecular characterization identification novel targeted therapies activity offer promise improved outcomes. To update...
Abstract Improved biomarkers are needed for early cancer detection, risk stratification, treatment selection, and monitoring response. Although proteins can be useful blood-based biomarkers, many have limited sensitivity or specificity these applications. Long INterspersed Element-1 (LINE-1) open reading frame 1 protein (ORF1p) is a transposable element overexpressed in carcinomas high-risk precursors during carcinogenesis with negligible expression normal tissues, suggesting ORF1p could...
Addition of ataxia telangiectasia and Rad3-related kinase inhibitors (ATRi) to PARP (PARPi) overcomes PARPi resistance in high-grade serous ovarian cancer (HGSOC) cell mouse models. We present the results an investigator-initiated study combination (olaparib) ATRi (ceralasertib) patients with acquired PARPi-resistant HGSOC.Eligible had recurrent, platinum-sensitive BRCA1/2 mutated or homologous recombination (HR)-deficient (HRD) HGSOC clinically benefited from (response by imaging/CA-125...