A5100422536- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- TGF-β signaling in diseases
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- S100 Proteins and Annexins
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Advanced Proteomics Techniques and Applications
- Retinoids in leukemia and cellular processes
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- PI3K/AKT/mTOR signaling in cancer
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Chromatin Remodeling and Cancer
- Connective Tissue Growth Factor Research
- Copper Interconnects and Reliability
- Wnt/β-catenin signaling in development and cancer
- Genomics and Phylogenetic Studies
Chengdu University of Traditional Chinese Medicine
2020-2024
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2024
Wenzhou Medical University
2024
The University of Texas Health Science Center at Houston
2024
Qingdao University
2022-2024
Kunming Medical University
2023-2024
University of North Carolina at Chapel Hill
2009-2024
Fuzhou University
2013-2024
Shenzhen University
2023-2024
Shenzhen Zhongshan Urological Hospital
2024
Several epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors have been developed and approved by Food Drug Administration for the treatment of non-small-cell lung cancers, but their efficacy can be compromised acquired drug resistance conferred EGFR-mutant variants. Here, we described discovery a novel E3 ligase von Hippel–Lindau-recruiting EGFR degrader, MS39 (compound 6), first-in-class cereblon-recruiting MS154 10), using proteolysis targeting chimera technology. These...
Spermatogenesis, a fundamental process in the male reproductive system, requires series of tightly controlled epigenetic and genetic events germ cells ranging from spermatogonia to spermatozoa. Jmjd1a is key regulator expressed testis. It specifically demethylates mono- di-methylated histone H3 lysine 9 (H3K9me1 H3K9me2) but not tri-methylated H3K9 (H3K9me3). In this study, we generated antibody for immunohistochemistry found was produced pachytene secondary spermatocytes. Disruption gene...
Many gene products exhibit great structural heterogeneity because of an array modifications. These modifications are not directly encoded in the genomic template but often affect functionality proteins. Protein glycosylation plays a vital role proper protein functions. However, analysis glycoproteins has been challenging compared with other modifications, such as phosphorylation. Here, we perform integrated proteomic and glycoproteomic 83 prospectively collected high-grade serous ovarian...
Osteogenic differentiation from mesenchymal progenitor cells (MPCs) are initiated and regulated by a cascade of signaling events. Either Wnt/β-catenin or estrogen pathway has been shown to play an important role in regulating skeletal development maintaining adult tissue homeostasis. Here, we investigate the potential crosstalk synergy these two pathways osteogenic MPCs. We find that activation receptor (ER) estradiol (E2) exogenously expressed ERα MPCs synergistically enhances Wnt3A-induced...
Increased amounts of reactive oxygen species (ROS) induce apoptosis in mammalian cells. PUMA (P53 up-regulated modulator apoptosis), a mitochondrial proapoptotic BH3-only protein, induces rapid through Bax- and mitochondria-dependent pathway. However, the molecular basis PUMA-induced is largely not understood. Using combination biophysical biochemical methods PUMA-inducible colorectal cells, DLD-1.PUMA, we showed that (a) dose time dependent; (b) directly associated with ROS generation; (c)...
Abstract Mesenchymal stem cells (MSCs) are multipotent progenitors and can differentiate into osteogenic, chondrogenic, adipogenic lineages. Bone morphogenetic proteins (BMPs) play important roles in cell proliferation differentiation. We recently demonstrated that BMP9 is a potent but less understood osteogenic factor. previously found BMP9‐induced ectopic bone formation not inhibited by BMP3. Here, we investigate the effect of BMP antagonist noggin on antagonists noggin, chording, gremlin,...
Osteosarcoma (OS) is the most common primary malignancy of bone and usually associated with poor prognosis due to its high incidence metastasis chemoresistance. Molecular pathogenesis OS poorly understood. We previously showed that cells are refractory BMP9-induced osteogenesis respond favorably proliferation tumor growth. Here we investigate if Notch signaling mediates BMP9-promoted cell growth human osteosarcoma (OS). find expression Notch1, Notch2, Notch3, DLL1, JAG1 JAG2 readily detected...
Perturbations in gene regulation during palatogenesis can lead to cleft palate, which is among the most common congenital birth defects. Here, we perform single-cell multiome sequencing and profile chromatin accessibility expression simultaneously within same cells (n = 36,154) isolated from mouse secondary palate across embryonic days (E) 12.5, E13.5, E14.0, E14.5. We construct five trajectories representing continuous differentiation of cranial neural crest-derived multipotent into...
Quorum sensing triggers virulence factor expression in medically important bacterial pathogens response to a density-dependent increase one or more autoinducing pheromones. Here, we show that phagocyte-derived oxidants target these autoinducers for inactivation as an innate defense mechanism of the host. In skin infection model, phagocyte NADPH oxidase, myeloperoxidase, inducible nitric oxide synthase was critical against quorum-sensing pathogen, Staphylococcus aureus , but not quorum...
The S100 protein family member S100A4 regulates various cellular functions.Previous studies have shown that elevated expression of is associated with progression and metastasis colorectal cancer (CRC).However, little known about whether how contributes to CRC development.In our present study, the in tissues compared matched adjacent normal was confirmed by immunohistochemistry, semi-quantitative RT-PCR Western blot.Adenovirus-mediated overexpression obviously enhanced viability migration...
By using DNA nuclease digestion and a quantitative "dual tagging" proteomic approach that integrated mass spectrometry, stable isotope labeling, affinity purification, we studied the histone H2AX-associating protein complex in chromatin mammalian cells response to ionizing radiation (IR). In non-irradiated control cells, calmodulin (CaM) transcription elongation factor facilitates (FACT) were associated with H2AX. Thirty minutes after exposing IR CaM FACT complexes dissociated, whereas two...
Cardiotoxicity is one of the leading causes drug attrition. Current in vitro models insufficiently predict cardiotoxicity, and there a need for alternative physiologically relevant models. Here we describe gene expression profile human-induced pluripotent stem cell–derived cardiocytes (iCC) postthaw over period 42 days culture compare this to human fetal adult as well cynomolgus nonhuman primate (NHP, Macaca fascicularis) heart tissue. Our results indicate that iCC express cardiac markers...
Taxol is a cytotoxic antiepithelioma chemotherapy drug widely used clinically, which results in appearing broad range of taxol-resistant tumors. Oncoprotein 18 (Op18)/stathmin genetically highly conserved small-molecule cytosolic phosphoprotein and expressed Extracellular signal-regulated kinase (ERK) main member mitogen-activated protein kinases (MAPKs). The study demonstrated that combination blockage ERK signal by inhibitor PD98059 greatly promoted taxol-induced cellular apoptosis growth...
Abstract Given the inherent limitations of current nano‐based antimicrobial materials, including their restricted broad‐spectrum efficacy and challenges in practical application, it is essential to develop an innovative therapeutic platform that effectively alleviates these shortcomings. Herein, inspired by cryptic properties natural marine mussels, o ‐dihydroxybenzene‐based covalent organic framework (TAPT‐2,3DHA‐COF) successfully synthesized, which exhibits potent antibacterial activity...
Mesenchymal stem cells (MSCs) are multipotent progenitors that can undergo osteogenic differentiation under proper stimuli. We demonstrated BMP9 is one of the most BMPs. However, molecular mechanism underlying BMP9-initiated signaling in MSCs remains unclear. Through gene expression profiling analysis we identified several candidate mediators signaling. Here, focus on such mediator and investigate functional role cysteine-rich with EGF-like domains 2 (Creld2) Creld2 was originally as an ER...
The S100A9 protein, a member of the S100 protein family, is often upregulated in various types cancer, including hepatocellular carcinoma (HCC). acts as danger signal when secreted to extracellular space and thought play an important role during tumorigenesis. Despite this fact, little known about effects tumor microenvironment on HCC. Therefore, study, we investigated exogenous proliferation invasion HepG2 HCC cells, well molecular mechanisms underlying these effects. Our results...
The S100A6 protein, a member of the S100 protein family, is overexpressed in many tumors including colorectal carcinoma (CRC). Although recent studies showed that elevated expression was associated with stage and lymphatic permeation CRC, little known about whether how contributes to CRC development. Here we investigated tissues cell lines, explored molecular mechanisms underlying role development by examining proliferation migration vitro, tumorigenicity nude mice. results show markedly...