A5100379337- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- Autophagy in Disease and Therapy
- Genetic Mapping and Diversity in Plants and Animals
- Protease and Inhibitor Mechanisms
- DNA Repair Mechanisms
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Psoriasis: Treatment and Pathogenesis
- Mitochondrial Function and Pathology
- Nitrogen and Sulfur Effects on Brassica
- Heavy Metal Exposure and Toxicity
- Smart Grid and Power Systems
- Nuclear Structure and Function
- Trace Elements in Health
- COVID-19 and healthcare impacts
- Molecular Biology Techniques and Applications
- S100 Proteins and Annexins
- Nerve injury and regeneration
- Histone Deacetylase Inhibitors Research
Huazhong University of Science and Technology
2015-2025
Ministry of Ecology and Environment
2015-2025
Shenzhen Nanshan Center for Chronic Disease Control
2024-2025
Oil Crops Research Institute
2025
Chinese Academy of Agricultural Sciences
2025
Tongji Hospital
2015-2024
ZheJiang Academy of Agricultural Sciences
2011-2024
Beth Israel Deaconess Medical Center
2013-2024
Harvard University
2013-2024
Fujian Provincial Hospital
2024
The Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB, rcsb.org), the US data center global PDB archive, serves thousands of Depositors in Americas and Oceania makes 3D macromolecular structure available at no charge without usage restrictions to more than 1 million rcsb.org Users worldwide 600 000 pdb101.rcsb.org education-focused around globe. include structural biologists using crystallography, nuclear magnetic resonance spectroscopy electron microscopy....
Abstract Background Osteoarthritis (OA) is a worldwide public health concern, mainly afflicting older adults. Although the etiology of OA remains unclear, environmental factors are increasingly considered as non-negligible risk factors. This study aims to evaluate associations urinary metals with and mediated effect biological aging. Methods Nine metal concentrations were detected among 12,584 U.S. adults based on National Health Nutrition Examination Survey (NHANES), including barium (Ba),...
Rad23 contains a ubiquitin-like domain (UbLR23) that interacts with catalytically active proteasomes and two ubiquitin (Ub)-associated (UBA) sequences bind Ub. The UBA domains can Ub in vitro, although the significance of this interaction vivo is poorly understood. interfere assembly multi-Ub chains high-level expression caused stabilization proteolytic substrates vivo. We report here ubiquitinated cellular proteins through synergistic action its domains. plays an overlapping role Rpn10,...
Background— The adaptation of cardiac mass to hemodynamic overload requires an protein turnover, ie, the balance between synthesis and degradation. We tested 2 hypotheses: (1) chronic left ventricular hypertrophy (LVH) activates proteasome system degradation, especially in myocardium submitted highest wall stress, subendocardium, (2) is required for development LVH. Methods Results— Gene expression subunits activity were measured separately from subendocardium subepicardium, right ventricle,...
Abstract The ubiquitin (Ub)/proteasome pathway facilitates the degradation of damaged proteins and regulators growth stress response. activation this in various cancers malignancies has been described, several genetic determinants breast cancer, including BRCA1 BRCA2, are linked to protein degradation. To investigate involvement Ub/proteasome system we examined a collection 25 patient-matched cancer normal adjacent tissues detected numerous components pathway. activity proteasome, levels...
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by renal cyst formation, inflammation, and fibrosis. Macrophages infiltrate cystic kidneys, but the role of these other inflammatory factors in progression are poorly understood. Here, we identified macrophage migration inhibitory factor (MIF) as an important regulator growth ADPKD. MIF was upregulated cyst-lining epithelial cells polycystin-1-deficient murine kidneys accumulated fluid human ADPKD kidneys. promoted cell...
Background The aetiology of diabetes is complex with limited treatment strategies. Growing animal studies have shown that targeted antiageing can improve the outcomes diabetes. However, population evidence limited. This study aims to evaluate associations biological ageing all-cause and cause-specific mortality among people Methods A total 5278 from National Health Nutrition Examination Survey 1999–2014 were included. Biological was measured different perspectives, including phenotypic age,...
Deubiquitinase-targeting chimeras (DUBTACs) have been recently developed to stabilize proteins of interest, which is in contrast targeted protein degradation (TPD) approaches that degrade disease-causing proteins. However, date, only the OTUB1 deubiquitinase has utilized develop DUBTACs via an covalent ligand, could unexpectedly compromise endogenous function owing its nature. Here, we show for first time USP7 can be harnessed DUBTAC development. Based on a noncovalent ligand USP7,...
Rad23 and Rpn10 play synergistic roles in the recognition of ubiquitinated proteins by proteasome, loss both causes growth proteolytic defects. However, physiological targets have not been well defined. We report that rad23Δ rpn10Δ is unable to grow presence translation inhibitors, this sensitivity was suppressed elongation factor 1A (eEF1A). This discovery suggested perform a role quality control. Certain inhibitors increase errors during protein synthesis cause release truncated...
Rad23 is a member of novel class proteins that contain unprocessed ubiquitin-like (UbL) domains. We showed recently small fraction can form an interaction with the 26S proteasome. Similarly, Rpn10 component bind multiubiquitin chains in vitro, but genetic studies have not clarified its role vivo. report here loss both and results pleiotropic defects are observed either single mutant. rad23Delta rpn10Delta displays slow growth, cold sensitivity, pronounced G2/M phase delay, implicating...
Mutations in alpha-synuclein, Parkin, and UCH-L1 cause heritable forms of Parkinson disease. Unlike for which no precise biochemical function has been elucidated, Parkin functions as a ubiquitin E3 ligase, is deubiquitinating enzyme. The ligase activity disease poorly understood further obscured by the fact that multiubiquitin chains can be formed through distinct types linkages regulate diverse cellular processes. For instance, lysine 48-linked target substrates to proteasome, whereas...
Increasing seed oil content is one of the most important goals in breeding rapeseed (B. napus L.). To dissect genetic basis B. napus, a large and new double haploid (DH) population containing 348 lines was obtained from cross between 'KenC-8' 'N53-2', two varieties with >10% difference content, this named KN DH population. A linkage map consisting 403 markers constructed, which covered total length 1783.9 cM an average marker interval 4.4 cM. The phenotyped eight natural environments...
Seed yield (SY) is the most important trait in rapeseed, determined by multiple seed yield-related traits (SYRTs) and also easily subject to environmental influence. Many quantitative loci (QTLs) for SY SYRTs have been reported Brassica napus; however, no studies focused on seven agronomic simultaneously affecting SY. Genome-wide QTL analysis eight environments was conducted a doubled haploid population containing 348 lines. Totally, 18 208 QTLs were observed, respectively, then these...
Yield of rapeseed is determined by three components: silique number, seed number per and thousand weight. Seed weight are influenced length, density, breadth, thickness volume. Some QTLs for traits have been reported in B. napus, however, no studies focused on the six agronomic (seed silique, thickness, density volume) simultaneously, genetic determinism such complex not fully elucidated.In this study, were evaluated using 348 lines a doubled haploid population, KN population. The results...
The targeting of proteolytic substrates is accomplished by a family ubiquitin-conjugating (E2) enzymes and diverse set substrate recognition (E3) factors.The ligation multiubiquitin chain to can promote its degradation the proteasome.However, mechanism that facilitates translocation proteasome in vivo poorly understood.We have discovered E2 proteins, including Ubc1, Ubc2, Ubc4, Ubc5, interact with 26S proteasome.Significantly, interaction between Ubc4 strongly induced heat stress, consistent...