A5054703440- Methane Hydrates and Related Phenomena
- Earthquake Detection and Analysis
- Seismic Waves and Analysis
- Protein purification and stability
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Astrophysics and Cosmic Phenomena
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Research in Cotton Cultivation
- Analytical Chemistry and Chromatography
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Photosynthetic Processes and Mechanisms
- Gut microbiota and health
- Plant Physiology and Cultivation Studies
- Gene expression and cancer classification
- Plant Pathogens and Fungal Diseases
Roche (United States)
2017-2025
Wuhan University
1997-2024
China Rehabilitation Research Center
2024
Capital Medical University
2024
Ministry of Agriculture and Rural Affairs
2023-2024
Renmin Hospital of Wuhan University
2023-2024
Liaocheng University
2024
Chinese Academy of Fishery Sciences
2023-2024
Pacific Northwest National Laboratory
2010-2023
Sichuan Agricultural University
2022
In this study, we evaluated a concatenated low pH (pH 3) and high 10) reversed-phase liquid chromatography strategy as first dimension for two-dimensional tandem mass spectrometry ("shotgun") proteomic analysis of trypsin-digested human MCF10A cell sample. Compared with the more traditional strong cation exchange method, use first-dimension fractionation resulted in 1.8- 1.6-fold increases number peptide protein identifications (with two or unique peptides), respectively. addition to broader...
Protein abundance and phosphorylation convey important information about pathway activity molecular pathophysiology in diseases including cancer, providing biological insight, informing drug diagnostic development, guiding therapeutic intervention. Analyzed tissues are usually collected without tight regulation or documentation of ischemic time. To evaluate the impact ischemia, we human ovarian tumor breast cancer xenograft tissue vascular interruption performed quantitative proteomics...
Orthogonal high-resolution separations are critical for attaining improved analytical dynamic range and protein coverage in proteomic measurements. High-pH reversed-phase liquid chromatography (RPLC), followed by fraction concatenation, affords better peptide analysis than conventional strong cation-exchange applied 2D analysis. For example, concatenated high-pH RPLC increased identification of peptides (by 1.8-fold) proteins 1.6-fold) shotgun proteomics analyses a digested human sample....
O -linked N -acetylglucosamine ( -GlcNAc) is a reversible posttranslational modification of Ser and Thr residues on cytosolic nuclear proteins higher eukaryotes catalyzed by -GlcNAc transferase (OGT). has recently been found Notch1 extracellular domain EGF domain-specific OGT. Aberrant brain linked to Alzheimer's disease (AD). However, understanding specific functions -GlcNAcylation in AD impeded the difficulty characterization sites proteins. In this study, we modified chemical/enzymatic...
In vitro transcription (IVT) of mRNA is a versatile platform for broad range biotechnological applications. Its rapid, scalable, and cost-effective production makes it compelling choice the development mRNA-based cancer therapies vaccines against infectious diseases. The impurities generated during can potentially impact safety efficacy therapeutics, but their structural complexity has not been investigated in detail yet. This study pioneers comprehensive profiling IVT impurities,...
Protein modification by O-linked β-N-acetylglucosamine (O-GlcNAc) is emerging as an important factor in the pathogenesis of sporadic Alzheimer's disease (AD); however, detailed molecular characterization this protein post-translational at proteome level has been highly challenging, owing to its low stoichiometry and labile nature. Herein, we report most comprehensive, quantitative proteomics analysis for O-GlcNAcylation postmortem human brain tissues with without AD use isobaric tandem mass...
We describe the preparation and performance of high-efficiency 70 cm × 20 μm i.d. silica-based monolithic capillary LC columns. The columns at a mobile-phase pressure 5000 psi provide flow rates ∼40 nL/min linear velocity ∼0.24 cm/s. separation peak capacity ∼420 in conjunction with both on-line coupling microsolid-phase extraction nanoelectrospray ionization-mass spectrometry. Performance was evaluated using Shewanella oneidensis tryptic digest, ∼15-amol detection limits for peptides were...
Cell fusion in genetically identical Neurospora crassa germlings and hyphae is a highly regulated process involving the activation of conserved MAP kinase cascade that includes NRC-1, MEK-2 MAK-2. During chemotrophic growth germlings, members localize to conidial anastomosis tube (CAT) tips every ∼8 minutes, perfectly out phase with another protein recruited tip: SOFT, recently identified scaffold for MAK-1 pathway Sordaria macrospora. How MAK-2 oscillation initiated, maintained what...
The ability of Leishmania to survive in their insect or mammalian host is dependent upon an sense and adapt changes the microenvironment. However, little known about molecular mechanisms underlying parasite response environmental changes, such as nutrient availability. To elucidate stress pathways donovani, we have used purine starvation paradigm. salvage purines from milieu obligatory for replication; nevertheless, purine-starved parasites can persist culture without supplementary over...
A hallmark of the response to high-dose radiation is up-regulation and phosphorylation proteins involved in cell cycle checkpoint control, DNA damage signaling, repair, apoptosis. Exposure cells low doses has well documented biological effects, but underlying regulatory mechanisms are still poorly understood. The objective this study provide an initial profile normal human skin fibroblast (HSF) phosphoproteome explore potential differences between low- irradiation responses at protein level....
Neurite extension and growth cone navigation are guided by extracellular cues that control cytoskeletal rearrangements. However, understanding the complex signaling mechanisms mediate neuritogenesis has been limited inability to biochemically separate neurite soma for spatial proteomic bioinformatic analyses. Here, we apply global proteome profiling in combination with a purification methodology comparative analysis of proteomes neuroblastoma cells. The relationship 4,855 proteins were...
A modular and adaptive mass spectrometry (MS)-based platform was developed to provide fast, robust sensitive host cell protein (HCP) analytics support process development. This relies on one-dimensional ultra-high performance liquid chromatography (1D UHPLC) combined with several different MS data acquisition strategies meet the needs of purification The workflow designed allow HCP composition quantitation for up 20 samples per day, a throughput considered essential real time bioprocess...
Cell movement requires morphological polarization characterized by formation of a leading pseudopodium (PD) at the front and trailing rear back. However, little is known about how protein networks are spatially integrated to regulate this process system level. Here, we apply global proteome profiling in combination with newly developed quantitative phosphoproteomics approaches for comparative analysis cell body (CB) PD chemotactic cells. The spatial relationship 3,509 proteins 228 distinct...
Protein tyrosine phosphorylation represents a central regulatory mechanism in cell signaling. Here, we present an extensive survey of sites normal-derived human mammary epithelial (HMEC) line by applying antiphosphotyrosine peptide immunoaffinity purification coupled with high sensitivity capillary liquid chromatography tandem mass spectrometry. A total 481 (covered 716 unique peptides) from 285 proteins were confidently identified HMEC following the analysis both basal condition and acute...
Spermatozoa are highly specialized cells that, when mature, capable of navigating the female reproductive tract and fertilizing an oocyte. The sperm cell is thought to be largely quiescent in terms transcriptional translational activity. As a result, once it has left male tract, essentially operating with static population proteins. It therefore theoretically possible understand protein networks contained deduce its cellular function capabilities. To this end, we performed proteomic analysis...
The NCI Clinical Proteomic Tumor Analysis Consortium (CPTAC) employed a pair of reference xenograft proteomes for initial platform validation and ongoing quality control its data collection Cancer Genome Atlas (TCGA) tumors. These two xenografts, representing basal luminal-B human breast cancer, were fractionated analyzed on six mass spectrometers in total 46 replicates divided between iTRAQ label-free technologies, spanning 1095 LC–MS/MS experiments. represent unique opportunity to evaluate...
The Systems Biology for Infectious Diseases Research program was established by the U.S. National Institute of Allergy and to investigate host-pathogen interactions at a systems level. This generated 47 transcriptomic proteomic datasets from 30 studies that in vivo vitro host responses viral infections. Human pathogens Orthomyxoviridae Coronaviridae families, especially pandemic H1N1 avian H5N1 influenza A viruses severe acute respiratory syndrome coronavirus (SARS-CoV), were investigated....
We demonstrated that the ten-eleven translocation (TET) dioxygenase-mediated oxidation of 5-methylcytosine followed by direct decarboxylation 5-carboxylcytosine constitutes a novel pathway for active DNA demethylation in mammalian genomes.