A5002318440- Immune Cell Function and Interaction
- interferon and immune responses
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- Diabetes and associated disorders
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Systemic Lupus Erythematosus Research
- Respiratory viral infections research
- Bioinformatics and Genomic Networks
- Salivary Gland Disorders and Functions
- Osteoarthritis Treatment and Mechanisms
- Cancer-related molecular mechanisms research
- Herpesvirus Infections and Treatments
- Immune responses and vaccinations
- Systemic Sclerosis and Related Diseases
- Cell Image Analysis Techniques
- Immune cells in cancer
- Adolescent and Pediatric Healthcare
- Genetics and Neurodevelopmental Disorders
- Gut microbiota and health
- Child Nutrition and Feeding Issues
Sorbonne Université
2011-2024
Inserm
2016-2024
Bipar
2019-2024
Sorbonne University Abu Dhabi
2023-2024
Centre d'Immunologie et des Maladies Infectieuses
2024
Assistance Publique – Hôpitaux de Paris
2020-2024
Hôpital Charles-Foix
2024
Délégation Ile-de-France Villejuif
2024
Pitié-Salpêtrière Hospital
2017-2023
Immunologie - Immunopathologie - Immunothérapie
2017-2022
Many enteropathogenic bacteria target the mammalian gut. The mechanisms protecting host from infection are poorly understood. We have studied protective functions of secretory antibodies (sIgA) and microbiota, using a mouse model for S. typhimurium diarrhea. This pathogen is common cause diarrhea in humans world-wide. (S. tmatt, sseD) causes self-limiting gut streptomycin-treated mice. After 40 days, all animals had overcome disease, developed sIgA response, most cleared lumen. limited...
Abstract Background Severe acute respiratory syndrome coronavirus-2 (SARS–CoV-2)-induced distress (ARDS) causes high mortality. Umbilical cord-derived mesenchymal stromal cells (UC-MSCs) have potentially relevant immune-modulatory properties, whose place in ARDS treatment is not established. This phase 2b trial was undertaken to assess the efficacy of UC-MSCs patients with SARS–CoV-2-induced ARDS. Methods multicentre, double-blind, randomized, placebo-controlled (STROMA–CoV-2) recruited...
The outcome of respiratory virus infection is determined by a complex interplay viral and host factors. Some potentially important factors for the antiviral response, whose functions remain largely unexplored, are long non-coding RNAs (lncRNAs). Here we systematically inferred regulatory lncRNAs in response to influenza A severe acute syndrome coronavirus (SARS-CoV) based on their similarity expression with genes known function. We performed total RNA-Seq viral-infected lungs from eight...
Chikungunya virus (CHIKV) is rapidly spreading across the globe, and millions are infected. Morbidity due to this a serious threat public health, but at present, there no vaccine against debilitating disease. We have recently developed number of candidates, here we evaluated 3 them in nonhuman primate model. A single immunization with an attenuated strain CHIKV (Δ5nsP3), homologous prime-boost DNA-launched RNA replicon encoding envelope proteins (DREP-E), DREP-E prime followed by recombinant...
Objective Primary Sjögren's syndrome (pSS) is characterised by chronic hyperactivation of B lymphocytes. Salivary gland epithelial cells (SGECs) could play a role in promoting B-lymphocyte activation within the target tissue. We aimed to study interactions between SGECs from patients with pSS or controls and Methods Patients had according 2016 European League Against Rheumatism/American College Rheumatology criteria. Gene expression analysis lymphocytes isolated salivary biopsies blood was...
Vaccination is one of the most efficient public healthcare measures to fight infectious diseases. Nevertheless, immune mechanisms induced in vivo by vaccination are still unclear. The route administration, an important parameter, can substantially modify quality response. How administration affects generation and profile responses major interest. Here, we aimed extensively characterize profiles innate adaptive response after intradermal, subcutaneous, or intramuscular with a modified...
Based on genetic associations, McGonagle and McDermott suggested a classification of autoimmune autoinflammatory diseases as continuum ranging from purely to comprising with both components. We used deep immunophenotyping identify immune cell populations molecular targets characterising this continuum.
Respiratory infections stemming from influenza viruses and the Severe Acute Syndrome corona virus (SARS-CoV) represent a serious public health threat as emerging pandemics. Despite efforts to identify critical interactions of these with host machinery, key regulatory events that lead disease pathology remain poorly targeted therapeutics. Here we implement an integrated network interrogation approach, in which proteome transcriptome datasets infection both human lung epithelial cells are...
Objective Primary Sjögren’s syndrome (SS) is characterized by a lymphocytic infiltration of salivary glands (SGs) and the presence an interferon (IFN) signature. SG epithelial cells (SGECs) play active role in primary SS pathophysiology. We undertook this study to examine interactions between SGECs T interleukin‐7 (IL‐7)/IFN axis. Methods cultured from control subjects patients with were stimulated poly(I‐C), IFNα, or IFNγ. sorted blood IL‐7. CD25 expression was assessed flow cytometry....
Modulating the host response is a promising approach to treating influenza, caused by virus whose pathogenesis determined in part reaction it elicits within host. Though pathogenicity of emerging H7N9 influenza several animal models has been reported, these studies have not included detailed characterization following infection. Therefore, we characterized transcriptomic BALB/c mice infected with (A/Anhui/01/2013) and compared responses induced H5N1 (A/Vietnam/1203/2004), H7N7...
The Systems Biology for Infectious Diseases Research program was established by the U.S. National Institute of Allergy and to investigate host-pathogen interactions at a systems level. This generated 47 transcriptomic proteomic datasets from 30 studies that in vivo vitro host responses viral infections. Human pathogens Orthomyxoviridae Coronaviridae families, especially pandemic H1N1 avian H5N1 influenza A viruses severe acute respiratory syndrome coronavirus (SARS-CoV), were investigated....
As for many model organisms, the amount of Listeria omics data produced has recently increased exponentially. There are now >80 published complete genomes, around 350 different transcriptomic sets, and 25 proteomic sets available. The analysis these through a systems biology approach generation tools biologists to browse various challenge bioinformaticians. We have developed web-based platform, named Listeriomics, that integrates analyses, i.e., (i) an interactive genome viewer display gene...
Gut microbiota dysbiosis is associated with inflammatory bowel diseases and cardiometabolic, neurological, autoimmune diseases. composition has a direct effect on the immune system, vice versa, it particular Treg homeostasis. Low-dose IL-2 (IL-2LD) stimulates Tregs promising treatment for We aimed to evaluate impact of IL-2LD gut correlatively system. used 16S ribosomal RNA profiling metagenomics characterize mice humans treated or not IL-2LD. performed fecal transplantation (FMT) from...
Abstract Motivation: Multidimensional scaling (MDS) is a well-known multivariate statistical analysis method used for dimensionality reduction and visualization of similarities dissimilarities in multidimensional data. The advantage MDS with respect to singular value decomposition (SVD) based methods such as principal component its superior fidelity representing the distance between different instances specially high-dimensional geometric objects. Here, we investigate importance choice...
The 2009 pandemic H1N1 influenza virus emerged in swine and quickly became a major global health threat. In mouse, non human primate, infection models, the pH1N1 efficiently replicates lung induces pro-inflammatory host responses; however, whether similar or different cellular pathways were impacted by across independent models remains to be further defined. To address this we have performed comparative transcriptomic analysis of acute phase responses single virus, A/California/04/2009...
Plasmodium falciparum malaria in India is characterized by high rates of severe disease, with multiple organ dysfunction (MOD)—mainly associated acute renal failure (ARF)—and increased mortality. The objective this study to identify cytokine signatures differentiating patients MOD, cerebral (CM), and MOD (CM-MOD) India. We have previously shown that two cytokines clusters differentiated CM from mild Maharashtra. Hence, we also aimed determine if these could discriminate subphenotypes Odisha....
The 1918 pandemic influenza virus was the most devastating infectious agent in human history, causing fatal pneumonia and an estimated 20 to 50 million deaths worldwide. Previous studies indicated a prominent role of hemagglutinin (HA) gene efficient replication high virulence mice. It is, however, still unclear whether ability or HA is required for exhibit Here, we examined biological properties reassortant viruses between contemporary H1N1 (A/Kawasaki/173/2001 [K173]) mouse model. In...
ABSTRACT The 1918-1919 influenza pandemic remains the single greatest infectious disease outbreak in past century. Mouse and nonhuman primate infection models have shown that 1918 virus induces overly aggressive innate proinflammatory responses. To understand response to viral role of individual genes on host virus, we examined reassortant avian viruses nearly identical (1918-like virus) carrying either hemagglutinin (HA) or PB2 gene. In mice, both enhanced 1918-like replication, but only...
Flow, hyperspectral and mass cytometry are experimental techniques measuring cell marker expressions at the single level. The recent increase of number markers simultaneously measurable has led to development new automatic gating algorithms. Especially, SPADE algorithm been proposed as a novel way identify clusters cells having similar phenotypes in high-dimensional data. While or other clustering algorithms powerful approaches, complementary analysis features needed better characterize...
Systems vaccinology allows cutting-edge analysis of innate biomarkers vaccine efficacy. We have been exploring novel strategies to shape the adaptive immune response, by targeting cells through immunization routes.This randomized phase I/II clinical study (n=60 healthy subjects aged 18-45 years old) used transcriptomic discover early response quality after transcutaneous (t.c.), intradermal (i.d.), and intramuscular (i.m.) administration a trivalent influenza (TIV season 2012-2013) (1:1:1...