A5073180895- Angiogenesis and VEGF in Cancer
- Cancer-related molecular mechanisms research
- Prostate Cancer Treatment and Research
- Reproductive Biology and Fertility
- Extracellular vesicles in disease
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Circular RNAs in diseases
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Sperm and Testicular Function
- Renal and related cancers
- Cancer, Lipids, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Glioma Diagnosis and Treatment
- Prostate Cancer Diagnosis and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
- Neurobiology and Insect Physiology Research
Université Paris-Est Créteil
2013-2023
Institut Cochin
2009-2022
Université Paris Cité
2011-2022
Inserm
2010-2022
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2016-2022
Université Paris-Saclay
2021-2022
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2022
CEA Paris-Saclay
2021-2022
Institut Mondor de Recherche Biomédicale
2019-2022
Centre National de la Recherche Scientifique
2004-2020
Castration resistance in prostate cancer (PCa) constitutes an advanced, aggressive disease with poor prognosis, associated uncontrolled cell proliferation, to apoptosis, and enhanced invasive potential. The molecular mechanisms involved the transition of PCa castration are obscure. Here, we report that nonselective cationic channel transient receptor potential vanilloid 2 (TRPV2) is a distinctive feature castration-resistant PCa. TRPV2 transcript levels were higher patients metastatic (stage...
Netrin-1 may promote colorectal and breast tumorigenesis, by inhibiting apoptosis induced its dependence receptors, deleted in cancer (DCC) uncoordinated-5-homolog (UNC5H). The status of netrin-1 receptors non-small cell lung (NSCLC) was unknown.The levels were analyzed a panel 92 NSCLC 25 human lines quantitative reverse transcription-polymerase chain reaction immunohistochemistry. In that express netrin-1, the expression inhibited using small interfering RNA (siRNA), or interference with...
Abstract The antitumor effects of pharmacologic inhibitors angiogenesis are hampered in patients by the rapid development tumor resistance, notably through increased invasiveness and accelerated metastasis. Here, we reevaluated role endogenous antiangiogenic thrombospondin 1 (TSP1) prostate carcinomas which is an active process. In xenografted tumors, observed that TSP1 altogether inhibited fostered development. Our results show a potent stimulator cell migration. This effect required CD36,...
Castration-resistant prostate cancers (CRPCs) that relapse after androgen deprivation therapies (ADTs) are responsible for the majority of mortalities from cancer (PCa). While mechanisms enabling recurrent activity receptor (AR) certainly involved in development CRPC, there may be factors contribute to process including acquired neuroendocrine (NE) cell-like behaviors working through alternate (non-AR) cell signaling systems or AR-dependent mechanisms. In this study, we explore potential...
Abstract Long noncoding (lnc)RNAs modulate gene expression alongside presenting unexpected source of neoantigens. Despite their immense interest, ability to be transferred and control adjacent cells is unknown. Extracellular Vesicles (EVs) offer a protective environment for nucleic acids, with pro antitumourigenic functions by controlling the immune response. In contrast extracellular nonvesicular RNA, few studies have addressed full RNA content within human fluids’ EVs compared them tissue...
Pea3 and Erm are transcription factors expressed in normal developing branching organs such as the mammary gland. Deregulation of their expression is generally associated with tumorigenesis particularly breast cancer. By using RNA interference (RNAi) to downregulate and/or a cancer cell line, we present evidence for role these proliferation, migration invasion capacity cells. We have used different small interfering RNAs (siRNAs) targeting pea3 erm transcripts transiently or stably...
// Sébastien Corbineau 1, 2, 3, 4 , Bruno Lassalle Maelle Givelet 4, 7, 8, 9 Inès Souissi-Sarahoui 6 Virginie Firlej Paul Henri Romeo Isabelle Allemand 5 Lydia Riou Pierre Fouchet 1 CEA DRF iRCM SCSR, Laboratoire de Recherche sur la réparation et Transcription dans les cellules Souches, UMR 967, F-92265 Fontenay-aux-Roses, France 2 INSERM, UMR967, 3 Université Paris Diderot, Sorbonne Cité, Sud, Gamétogenèse, Apoptose Génotoxicité, Radiopathologie, 7 INSERM U1016, Institut Cochin, 75014, 8...
Abstract Small Extracellular Vesicles (sEVs) are 50–200 nm in diameter vesicles delimited by a lipid bilayer, formed within the endosomal network or derived from plasma membrane. They secreted various biological fluids, including airway nasal mucus. The goal of this work was to understand role sEVs present mucus (mu‐sEVs) produced human epithelial cells (HNECs) SARS‐CoV‐2 infection. We show that uninfected HNECs produce mu‐sEVs containing receptor ACE2 and activated protease TMPRSS2. cleave...
Markers of prostate tumor recurrence after radical prostatectomy are lacking and highly demanded. The androgen receptor (AR) is a nuclear that plays pivotal role in normal cancerous tissue. AR interacts with number proteins modulating its stability, localization, activity. To test the hypothesis an increased expression partners might foster development, we immunopurified human tumors xenografted into mice. One identified was multifunctional enzyme carbamoyl-phosphate synthetase II, aspartate...
Recurrent spontaneous abortion (RSA) is defined as the loss of three or more consecutive pregnancies during first trimester embryonic intrauterine development. This kind human infertility frequent among general population since it affects 1 to 5% women. In half cases etiology remains unelucidated. present study, we used interspecific recombinant congenic mouse strains (IRCS) in aim identify genes responsible for lethality. Applying a cartographic approach using genotype/phenotype...
The use of RNA-sequencing technologies held a promise improved diagnostic tools based on comprehensive transcript sets. However, mining human transcriptome data for disease biomarkers in clinical specimens are restricted by the limited power conventional reference-based protocols relying unique and annotated transcripts. Here, we implemented blind reference-free computational protocol, DE-kupl, to infer yet unreferenced RNA variations from total stranded datasets tissue origin. As bench...
Numerous pan-genomic studies identified alterations in protein-coding genes and signaling pathways involved bladder carcinogenesis, while non-coding somatic remain weakly explored. The goal of this study was to identify clinical biomarkers regions for cancer patients. We have previously tumors two mutational hotspots occurring at high frequencies (≥30%). These mutations are located close the GPR126 PLEKHS1 genes, guanine or cytosine a TGAACA core motif flanked, on both sides, by stretch...
Prostate cancer (PCa) is the second most frequent and fifth leading cause of death in men worldwide. If local PCa presents a favorable prognosis, available treatments for advanced display limiting benefits due to therapeutic resistances. Nucleolin (NCL) ubiquitous protein involved numerous cell processes, such as ribosome biogenesis, cycles, or angiogenesis. NCL overexpressed several tumor types which it has been proposed diagnostic prognostic biomarker. In PCa, mainly studied target new...
Abstract Background Neuroendocrine prostate cancer (NEPC) is a multi-resistant variant of (PCa) that has become major challenge in clinics. Understanding the neuroendocrine differentiation (NED) process at molecular level therefore critical to define therapeutic strategies can prevent multi-drug resistance. Methods Using RNA expression profiling and immunohistochemistry, we have identified characterised gene signature associated with emergence NED large PCa cohort, including 169...
The Pea3 transcription factor (which belongs to the PEA3 group) from Ets family has been shown be involved in mammary embryogenesis and oncogenesis. However, except for proteinases, only few of its target genes have reported. In present report, we identified bax as a up-regulated gene. We provide evidence this regulation by using overexpression silencing cell line. Both Erm, another member group, are able transactivate promoter fragments. Although minimal Pea3-regulated does not contain an...
RNA-seq data are increasingly used to derive prognostic signatures for cancer outcome prediction. A limitation of current predictors is their reliance on reference gene annotations, which amounts ignoring large numbers non-canonical RNAs produced in disease tissues. recently introduced kind transcriptome classifier operates entirely a reference-free manner, relying k-mers extracted from patient data.In this paper, we set out compare conventional and risk relapse prediction prostate cancer....
Fanconi anemia (FA) is a rare human genetic disorder characterized by bone marrow failure, predisposition to cancer and developmental defects including hypogonadism. Reproductive leading germ cell aplasia are the most consistent phenotypes seen in FA mouse models. We examined role of nuclear core complex gene Fancg development primordial cells (PGCs), embryonic precursors adult gametes, during fetal development. PGC maintenance was severely impaired Fancg-/- embryos. observed defect number...
Abstract B-lymphocytes in the bone marrow (BM) must generate a functional B-cell receptor and overcome negative selection induced by reactivity with autoantigens. Two rounds of DNA recombination are required for production immunoglobulin heavy (Ig-HCs) light (LCs) chains necessary continuation B-lymphocyte development BM. Both depend on joint action activating gene-1 (RAG-1) RAG-2 endonucleases non-homologous end-joining pathway. Loss FANC gene leads to chromosome breakage cancer...
Male infertility is responsible for approximately half of all cases reproductive issues. Spermatogenesis originates in a small pool spermatogonial stem cells (SSCs), which are interest therapy but remain not well defined humans. Using multiparametric analysis the side population (SP) phenotype and α-6 integrin, THY1, β-2 microglobulin cell markers, we identified human primitive undifferentiated spermatogonia with (β-2M)−SPα-6+THY1+, highly enriched cells. By analyzing expression signatures...