A5033494288- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Renal and related cancers
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic factors in colorectal cancer
- Hemoglobinopathies and Related Disorders
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Wastewater Treatment and Nitrogen Removal
- Genomic variations and chromosomal abnormalities
- Hematopoietic Stem Cell Transplantation
- Legume Nitrogen Fixing Symbiosis
- Telomeres, Telomerase, and Senescence
- RNA modifications and cancer
- Genetic Mapping and Diversity in Plants and Animals
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2010-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2010-2024
Université Paris Cité
2012-2024
Institut de Radiobiologie Cellulaire et Moléculaire
2009-2024
Inserm
2006-2024
CEA Paris-Saclay
2019-2024
Université Paris-Saclay
2011-2024
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2019-2023
Stabilité Génétique, Cellules Souches et Radiations
2015-2023
Délégation Paris 7
2008-2019
Abstract Background Spermatogenesis in adult is a complex stepwise process leading to terminally differentiated spermatozoa. The cellular heterogeneity of testis renders the studies on molecular aspects this differentiation process. Analysis regulation spermatogenesis would undoubtedly benefit from development techniques characterize each germinal step. Methods Hoechst 33342 staining mouse testicular cells allows characterization an enriched population stem cell and spermatogonia, called...
Abstract The Polycomb group of proteins is required for the proper orchestration gene expression due to its role in maintaining transcriptional silencing. It composed several chromatin modifying complexes, including Repressive Complex 2 (PRC2), which deposits H3K27me2/3. Here, we report identification a cofactor PRC2, EZHIP (EZH1/2 Inhibitory Protein), expressed predominantly gonads. limits enzymatic activity PRC2 and lessens interaction between core complex accessory subunits, but does not...
Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be identified by the 'Side Population' marker based on Hoechst 33342 efflux. We show that mouse testicular also display a express Bcrp1 mRNA, ABC transporter responsible for efflux hematopoietic cells. Inhibition of specific BCRP1 inhibitor Ko143 germinal phenotype is dependent activity. Analysis two well-defined models altered spermatogenesis (W/Wv mutants and cryptorchid male mice) RNA expression studies...
Few techniques are available to characterize in vivo the early cellular dynamics of long-term reconstitution hematopoiesis after transplantation hematopoietic stem cells (HSCs) lethal irradiation. Using a fiber-optic imaging system, we track steps recruitment and proliferation Lin(-)Sca-1(+)c-Kit(+)CD34(-) (LSKCD34(-)) HSCs highly enriched transplanted into lethally irradiated mice. Recruitment LSKCD34(-) first occurs femoral head is continuous during 24 hours. Quantification fluorescence...
Anti-silencing function 1 (ASF1) is an evolutionarily conserved histone H3-H4 chaperone involved in the assembly/disassembly of nucleosome and modification. Two paralogous genes, Asf1a Asf1b, exist mouse genome. ubiquitously expressed its loss causes embryonic lethality. Conversely, Asf1b expression more restricted has been less studied. To determine vivo we generated a Asf1b-deficient line (Asf1b(GT(ROSA-βgeo)437)) which lacZ reporter gene driven by promoter. Analysis β-galactosidase...
Abstract Human and swine chromosomes were analyzed separately as a mix to obtain bivariate flow karyotypes. They normalized each other in order use the human chromosomal DNA content standard. Our results led characterization of “DNA line” identical line.” Estimation megabase pairs is proposed. Chromosomal assignment various resolved peaks on karyotype suggested from relation between quantified by cytometry size. Swine 1, 13, 6, 5, 10, 16, 11, 18, Y assigned A, B, C, K, L, N, O, Q, Y,...
Abstract Developing and adult forebrains contain neural stem cells (NSCs) but no marker is available to highly purify them. When analysed by flow cytometry, from various tissues are enriched in a ‘side population’ (SP) characterized the exclusion of fluorescent dye Hoechst 33342. Here, we characterize SP embryonic, neonatal forebrains, as well neurosphere cultures have determined whether this could be source NSCs. By using specific inhibitors, found that embryonic forebrain results activity...
// Sébastien Corbineau 1, 2, 3, 4 , Bruno Lassalle Maelle Givelet 4, 7, 8, 9 Inès Souissi-Sarahoui 6 Virginie Firlej Paul Henri Romeo Isabelle Allemand 5 Lydia Riou Pierre Fouchet 1 CEA DRF iRCM SCSR, Laboratoire de Recherche sur la réparation et Transcription dans les cellules Souches, UMR 967, F-92265 Fontenay-aux-Roses, France 2 INSERM, UMR967, 3 Université Paris Diderot, Sorbonne Cité, Sud, Gamétogenèse, Apoptose Génotoxicité, Radiopathologie, 7 INSERM U1016, Institut Cochin, 75014, 8...
The stem cell niche plays a crucial role in the decision to either self-renew or differentiate. Recent observations lead hypothesis that O 2 supply by blood and local tension could be key components of testicular spermatogonial cells (SSCs). In this study, we investigated impact different hypoxic conditions (3.5%, 1%, 0.1% tension) on murine human SSCs culture. We observed deleterious effect severe hypoxia (1% ) capacity form germ clusters when plated at low density. Severe effects...
Several somatic genetic alterations have been described in non-small-cell lung carcinomas (NSCLC). Recurrent chromosomal deletions suggested the presence of tumor-suppressor genes specifically involved carcinogenesis. For one these, 2 non-overlapping regions proposed on short arm chromosome 8, encompassing LPL and NEFL genes. The region has extensively studied NSCLC other cancer types. Two genes, N33 PRLTS, identified, but small number mutations excludes their involvement vast majority...
Fanconi anemia (FA) is a human rare genetic disorder characterized by congenital defects, bone marrow (BM) failure and predisposition to leukemia. The progressive aplastic suggests defect in the ability of hematopoietic stem cells (HSC) sustain hematopoieis. We have examined role nuclear FA core complex gene Fancg functionality HSC. In Fancg−/− mice, we observed decay long-term HSC multipotent progenitors that account for reduction LSK compartment containing primitive cells. lymphoid myeloid...
Abstract Testis is one of the organs with most telomerase activity in adult. This protects chromosomes from telomere attrition and ensures transmission full-length to progeny. Little known about during adult germ cell differentiation, however. We demonstrate here that mouse testis resides α6-integrin-positive Side Population containing spermatogonia enriched spermatogonial stem cells. The these cells fell upon entry into meiosis subsequent spermiogenesis. In addition, various stages...
AbstractDefects in DNA repair pathways have been involved collapse of early neurogenesis leading to brain development abnormalities and embryonic lethality. However, consequences defects adult neural stem progenitor cells their potential contribution ageing phenotype are poorly understood. The Fanconi anaemia (FA) pathway, which functions primarily as a damage response system, has examined during developmental neurogenesis. We shown that loss fanca fancg specifically provokes apoptosis...