A5077662799- Estrogen and related hormone effects
- Hormonal Regulation and Hypertension
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- Aldose Reductase and Taurine
- Telomeres, Telomerase, and Senescence
- Colorectal Cancer Treatments and Studies
- Prostate Cancer Treatment and Research
- Animal Genetics and Reproduction
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Nuclear Receptors and Signaling
- PI3K/AKT/mTOR signaling in cancer
- Macrophage Migration Inhibitory Factor
- Hormonal and reproductive studies
- Genomics and Chromatin Dynamics
- Circadian rhythm and melatonin
- Cancer-related Molecular Pathways
- Galectins and Cancer Biology
- Protein Degradation and Inhibitors
- T-cell and Retrovirus Studies
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- RNA modifications and cancer
Institut Pasteur de Lille
2012-2025
Inserm
1997-2025
Centre National de la Recherche Scientifique
2013-2025
Université de Lille
2011-2025
Centre Hospitalier Universitaire de Lille
2023-2024
Université Lille Nord de France
2010-2023
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2023
Institut de Biologie de Lille
2008-2019
Mécanismes de Tumorigenèse et Thérapies Ciblées
2012-2018
Université Libre de Bruxelles
1992-2017
The 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3βHSD) enzyme catalyzes the oxidation and isomerization of Δ5−3β-hydroxysteroid precursors into Δ4-ketosteroids, thus leading to formation all classes steroid hormones. In addition, 3βHSD interconversion 3β-hydroxy- 3-keto-5α-androstane steroids. Clinical observations in patients with deficiency as well our recent data obtained by Southern blot analysis using a human placental cDNA (type I) probe suggested existence multiple related...
Regulatory T cells (Treg) and tumor-exosomes are thought to play a role in preventing the rejection of malignant patients bearing nasopharyngeal carcinoma (NPC). Treg recruitment by exosomes derived from NPC cell lines (C15/C17-Exo), isolated patients' plasma (Patient-Exo), CCL20 were tested vitro using Boyden chamber assays vivo xenograft SCID mouse model (n = 5), both presence absence anti-CCL20 monoclonal antibodies (mAb). Impact these (NPC-Exo) on phenotype function was determined...
The persistence of transcriptionally silent but replication-competent HIV-1 reservoirs in Highly Active Anti-Retroviral Therapy (HAART)-treated infected individuals, represents a major hurdle to virus eradication. Activation gene expression these cells together with an efficient HAART has been proposed as adjuvant therapy aimed at decreasing the pool latent viral reservoirs. Using latently-infected U1 monocytic cell line and J-Lat T-cell clones, we here demonstrated strong synergistic...
Reactions of oestrogens and androgens at position C-17 are catalysed by 17 beta-hydroxysteroid dehydrogenases (17 beta-HSDs). Cloning the cDNA a novel human beta-HSD IV expression its mRNA described. A probe derived from recently discovered porcine beta-oestradiol dehydrogenase beta-EDH) was used to isolate 2.6 kb encoding continuous protein 736 amino acids high (84%) similarity beta-EDH. The calculated molecular mass enzyme is 79,595 Da. Other sequence similarities shared two enzymes are:...
Abstract Three beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase (3 beta-HSD) catalyze the oxidative conversion of delta 5-3 beta-hydroxysteroids to 4-3-keto configuration and is therefore essential for biosynthesis all classes hormonal steroids, namely progesterone, glucocorticoids, mineralocorticoids, androgens, estrogens. Using human 3 beta-HSD cDNA as probe, a gene was isolated from lambda-EMBL3 library leucocyte genomic DNA. A fragment DNA also obtained by amplification using...
We show that high-grade astrocytic tumors with high levels of galectin-1 expression are associated dismal prognoses. The immunohistochemical analysis human U87 and U373 glioblastoma xenografts from the brains nude mice revealed a higher level in invasive areas rather than non-invasive xenografts. Nude intracranially grafted or cells constitutively expressing low (by stable transfection an vector containing antisense mRNA galectin-1) had longer survival periods those normal galectin-1....
Abbreviations and trivial names used are: 3p-HSD, 30-hydroxysteroid dehydrogenase (EC 1.1.1.145)/A5-A4isomerase 5.3.3.1);PREG, pregnenolone (5-pregnene-38-01-20-one); DHEA, dehydroepiandrosterone (androst-5-ene-3P-ol-17-one); a"-diol (androst-5-ene-3P,178-diol); PROG, progesterone (4-pregnene-3,20-dione); A'-dione, androstenedione (androst-4-ene-3,17-dione); testosterone (androst-4ene-170-01-3-one); DHT, 5a-dihydrotestosterone (5a-
TRPM8 is a cold sensor that highly expressed in the prostate as well other non-temperature-sensing organs, and regulated by downstream receptor-activated signaling pathways. However, little known about intracellular proteins necessary for channel function. Here, we identify two previously unknown proteins, which have named "TRP channel-associated factors" (TCAFs), new partner demonstrate they are TCAF1 TCAF2 both bind to promote its trafficking cell surface. exert opposing effects on gating...
Matrix metalloproteinases belong to a family of structurally related enzymes that plays important role in tissue morphogenesis, differentiation, and wound healing. Their expression is negatively regulated by several members the steroid hormone receptor family. This thought occur through interaction receptors with transcription factor AP-1 otherwise required for positive regulation. Here, we demonstrate not always target down-regulation matrix receptors. Androgen regulates metalloproteinase-1...
Abstract Metastasis and drug resistance are major problems in cancer chemotherapy. The purpose of this work was to analyze the molecular mechanisms underlying invasive potential drug-resistant colon carcinoma cells. Cellular models included parental HT-29 cell line its derivatives selected after chronic treatment with either 5-fluorouracil, methotrexate, doxorubicin, or oxaliplatin. Drug-resistant cells were compared noninvasive using cDNA microarray, quantitative reverse transcription-PCR,...
Background Liver transplantation is the treatment of end-stage liver diseases, including hepatitis C. Immunosuppression prevents graft rejection but seems to accelerate recurrence Regulatory T cells (Tregs) may be beneficial in tolerance deleterious recurrent We evaluated effects cyclosporine or tacrolimus, principal immunosuppressive drugs, on Treg proliferation and function. Methods Human Tregs were isolated from healthy donors cultured with cyclosporine, NIM811, a analog devoid...
Abstract Studies on human fibroblasts have led to viewing senescence as a barrier against tumorigenesis. Using keratinocytes, we show here that partially transformed and tumorigenic cells systematically spontaneously emerge from senescent cultures. We these emerging are generated cells, which still competent for replication, by an unusual budding-mitosis mechanism. further present data implicating reactive oxygen species accumulate during potential mutagenic motor of this post-senescence...
Inflammatory bowel diseases (IBDs) are chronic inflammatory of the gastrointestinal tract, which clinically present as 1 2 disorders, Crohn's disease (CD) or ulcerative colitis (UC) (Rogler 2004). The immune dysregulation in intestine plays a critical role pathogenesis IBD, involving wide range molecules, including cytokines. aim this work was to study involvement T-helper 17 (Th17) subset by nitric oxide (NO) pathway Algerian patients with IBD. We investigated correlation between...
DNA methylation is a central epigenetic modification that established by de novo methyltransferases. The mechanisms underlying the generation of genomic patterns are still poorly understood. Using mass spectrometry and phosphospecific Dnmt3a antibody, we demonstrate CK2 phosphorylates endogenous at two key residues located near its PWWP domain, thereby downregulating ability to methylate DNA. Genome-wide analysis shows primarily modulates CpG several repeats, most notably Alu SINEs. This...
Colitis associated cancer (CAC) is the colorectal (CRC) subtype that with bowel disease such as ulcerative colitis (UC). The data on role of NF-κB signaling in development and progression CAC were derived from preclinical studies, whereas human are rare. aim this work was to study contribution pathway during UC CAC, well immunomodulatory effect all-trans retinoic acid (AtRA). We analyzed expression NOS2, TNF-α, TLR4, NF-κB, colonic mucosa. also studied NO/TNF-α modulation by LPS mucosa...
The ETS transcription factor ETV4 is involved in the main steps of organogenesis and also a significant mediator tumorigenesis metastasis, such as breast cancer. Indeed, overexpressed tumors associated with distant metastasis poor prognosis. However, cellular molecular events regulated by this are still misunderstood. In mammary epithelial cells, controls expression many genes, MMP13 among them. aim study was to understand function during ETV4-driven tumorigenesis. Different constructs gene...
ABSTRACT The transcription factor NF-κB plays a central role in the human immunodeficiency virus type 1 (HIV-1) activation pathway. HIV-1 is also regulated by protein acetylation, since treatment with deacetylase inhibitors such as trichostatin A (TSA) or sodium butyrate (NaBut) markedly induces transcriptional activity of long terminal repeat (LTR) promoter. Here, we demonstrate that TSA synergized both ectopically expressed p50/p65 and tumor necrosis alpha/SF2 (TNF)-induced to activate...