A5018151806- Receptor Mechanisms and Signaling
- Helicobacter pylori-related gastroenterology studies
- Digestive system and related health
- Immune Cell Function and Interaction
- Parasites and Host Interactions
- Acute Myocardial Infarction Research
- IL-33, ST2, and ILC Pathways
- Microscopic Colitis
- Inflammatory Bowel Disease
- Estrogen and related hormone effects
- Gut microbiota and health
- Peroxisome Proliferator-Activated Receptors
- Ion channel regulation and function
- Clinical Nutrition and Gastroenterology
- Chromosomal and Genetic Variations
- Coronary Interventions and Diagnostics
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Cardiac Imaging and Diagnostics
- Retinoids in leukemia and cellular processes
- Drug Transport and Resistance Mechanisms
- Nuclear Receptors and Signaling
- Breastfeeding Practices and Influences
- Trypanosoma species research and implications
- Pancreatic and Hepatic Oncology Research
Inserm
2011-2025
Université de Lille
2008-2025
Lille Inflammation Research International Center
2015-2025
Centre Hospitalier Universitaire de Lille
2013-2025
Institute for Translational Research in Inflammation
2023-2025
Sorbonne Université
1993-2023
Assistance Publique – Hôpitaux de Paris
2019-2023
Pitié-Salpêtrière Hospital
2011-2023
Limagrain (France)
2023
Genethon (France)
2023
Signaling through serotonin 5-HT1A receptors involves multiple pathways. We have investigated the functional coupling of human receptor to different G proteins using an in vitro reconstitution system based on expression recombinant (r5-HT1A) and alpha-subunits (rG alpha) Escherichia coli. The r5-HT1A was expressed by insertion a vector allowing its active E. coli inner membranes. Binding selective agonist [3H] +/- 8-hydroxy-(2-N-dipropylamine)tetralin ([3H]8-OH-DPAT) intact bacteria or...
Intestinal fibrosis is mainly associated with Crohn's disease and defined as a progressive excessive deposition of extracellular matrix components. No specific antifibrotic therapies are available. In this study, we evaluate the effect novel 5-ASA analog able to activate peroxisome proliferator-activated receptor γ, named GED-0507-34 Levo.Colonic was induced in 110 C57BL/6 mice by 3 cycles 2.5% (wt/vol) dextran sulfate sodium administration for 6 weeks. The preventive effects oral daily GED...
DNA methylation is a central epigenetic modification that established by de novo methyltransferases. The mechanisms underlying the generation of genomic patterns are still poorly understood. Using mass spectrometry and phosphospecific Dnmt3a antibody, we demonstrate CK2 phosphorylates endogenous at two key residues located near its PWWP domain, thereby downregulating ability to methylate DNA. Genome-wide analysis shows primarily modulates CpG several repeats, most notably Alu SINEs. This...
The purified bovine brain A,-adenosine receptor has previously been shown to discriminate among closely related G protein a-subunits.To obtain analogous information for the human receptor, cDNA coding was inserted into a plasmid placing synthesis of under control MalE promoter.Following induction by maltose, active accumulated in Escherichia coli membranes.Binding antagonist S-~3Hlcyclopentyl-l,3-dipropylxanthine E. membranes (K, -2 MI, B,, -0.2-0.4 pmoYmg) showed appropriate pharmacological...
Cytosine-phosphate-guanine (CpG) motifs in bacterial DNA are known to activate the mammalian immune system, and this activation is thought depend on Toll-like receptor 9 (TLR9) signaling pathway. Previous studies strongly suggested that TLR9 involved as specific for CpG but did not provide direct evidence of their interaction. In study, we demonstrate first time murine binds an unmethylated CpG-containing plasmid. This interaction sequence-specific influenced by methylation status...
The consequences of agonist-dependent activation guanine nucleotide-binding protein (G protein)-coupled receptors vary from cell to cell, depending on a complex network regulations between components the signaling cascade. Specific interactions receptors, G proteins, and effectors are difficult analyze in intact cells. Engineering receptor-transducer fusion proteins might be an effective strategy target cellular more efficiently specifically. As model, we evaluated ability beta 2-adrenergic...
Retinoid X receptors (RXR) are members of the nuclear receptor superfamily ligand-activated transcription factors that have been characterized in a wide variety metazoan phyla. They act as heterodimer partners other receptors, and vertebrates also activate homodimers presence ligand, 9 -cis retinoic acid. In order to test hypothesis acid signaling pathways involving RXRs present Lophotrochozoa, we sought isolate conserved this family from platyhelminth parasite Schistosoma mansoni its...
<h3>Background and aims</h3> Immune tolerance breakdown during UC involves the peroxisome proliferator-activated receptor-γ (PPARγ), a key factor in mucosal homoeostasis therapeutic target of 5-aminosalycilates, which expression is impaired UC. Here we assess impact glucocorticoids (GCs) on PPARγ expression, focusing especially extra-adrenal cortisol production by colonic epithelial cells (CECs). <h3>Methods</h3> Activation colon was evaluated using transgenic mice for luciferase gene under...
Abstract The gastrointestinal tract is a remarkable example of complex biology, with constant dialogue between the intestinal epithelium, in close contact microbiota, and immune cells that protect gut from infection. Organoids have revolutionized our approach to modelling cellular compartment opened new avenues for unravelling mechanisms involved homeostasis chronic pathogenesis such as inflammatory bowel disease. To date, few models been established explore role colon, which however main...
Vanin-1 is an epithelial pantetheinase, which regulates intestinal inflammation in mouse. We investigated whether human VNN1 levels could be associated to the susceptibility inflammatory bowel diseases (IBD) and explored participation of PPARg these processes.We studied expression colon biopsies from IBD patients. polymorphisms regulatory regions gene examined their genetic association with disease. Functional relevance single-nucleotide (SNPs) was assayed, we tested nuclear complexes...
Heart rate variability (HRV) depends on various reflexes, including the baroreflex or respiratory reflex. Experimental studies have suggested that sinoatrial node density in G protein-linked receptors may be involved. Transgenic mice, with a specific eightfold atrial overexpression of human beta 1-adrenoceptor (beta 1-AR), been generated to evaluate role 1-AR HRV. The heart was monitored using telemetry, and signal analyzed quantitative time-frequency domain analysis, smoothed...
Cigarette smoke (CS) protects against intestinal inflammation during ulcerative colitis. Immunoregulatory mechanisms sustaining this effect remain unknown. The aim of study was to assess the effects CS on experimental colitis and characterize inflammatory response at cellular molecular levels. Using InExpose® System, a smoking device accurately reproducing human habit, we pre-exposed C57BL/6 mice for 2 weeks CS, then induced by administration dextran sodium sulfate (DSS). This system allowed...
Epidemiological evidences suggested that 5-aminosalicylic acid (5-ASA) therapy may prevent the development of colorectal cancer in inflammatory bowel disease patients. Our aim is to investigate whether peroxisome proliferator-activated receptor-γ (PPARγ) mediates antineoplastic effects 5-ASA. HT-29 and Caco-2 cells were treated by 5-ASA, rosiglitazone (PPARγ ligand) or etoposide (anticarcinogenic drug). Epithelial cell growth, proliferation apoptosis assessed count, Ki-67 staining terminal...
Pompe disease (PD) is a neuromuscular disorder caused by acid α-glucosidase (GAA) deficiency. Reduced GAA activity leads to pathological glycogen accumulation in cardiac and skeletal muscles responsible for severe heart impairment, respiratory defects, muscle weakness. Enzyme replacement therapy with recombinant human (rhGAA) the standard-of-care treatment PD, however, its efficacy limited due poor uptake development of an immune response. Multiple clinical trials are ongoing PD...
Most Drosophila transposable elements are LTR retrotransposons, some of which belong to the genus Errantivirus and share structural functional characteristics with vertebrate endogenous retroviruses. Like retroviruses, it is unclear whether errantiviruses retain infectivity transposition capacity. We created conditions where control ZAM errantivirus through piRNA pathway was abolished leading its de novo reactivation in somatic gonadal cells. After reactivation, invaded oocytes severe...
The first 1000 days of life is a critical period that contributes significantly to the programming an individual's future health. Among many changes occur during this early in life, there growing evidence establishment healthy gut microbiota plays important role prevention both short- and long-term health problems. Numerous publications suggest quality colonisation depends on several dietary factors, including breastfeeding. In respect, relationship between breastfeeding risk inflammatory...
The fushi tarazu factor 1 (FTZ‐F1) nuclear receptor subfamily comprises orphan receptors with crucial roles in development and sexual differentiation vertebrates invertebrates. We describe the structure functional properties of an FTZ‐F1 from platyhelminth parasite humans, Schistosoma mansoni , first this family to be characterized a Lophotrochozoan. It contains well conserved DNA‐binding domain (55–63% identity other members) poorly ligand‐binding (20% that zebrafish FF1a). However, both...