A5077311708- Pancreatic function and diabetes
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Parasites and Host Interactions
- Cancer-related gene regulation
- Diabetes Treatment and Management
- Estrogen and related hormone effects
- Diabetes and associated disorders
- Genomics and Chromatin Dynamics
- Nuclear Receptors and Signaling
- Protein Degradation and Inhibitors
- Peroxisome Proliferator-Activated Receptors
- Genetics and Neurodevelopmental Disorders
- Birth, Development, and Health
- Cancer, Lipids, and Metabolism
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Drug Transport and Resistance Mechanisms
- Error Correcting Code Techniques
- Mass Spectrometry Techniques and Applications
- Peptidase Inhibition and Analysis
- Cholinesterase and Neurodegenerative Diseases
Inserm
2011-2024
Université de Lille
2012-2024
Institut Pasteur de Lille
2011-2024
Centre Hospitalier Universitaire de Lille
2017-2024
Centre National de la Recherche Scientifique
2009-2024
European Genomic Institute for Diabetes
2013-2023
(Epi)génomique fonctionnelle métabolique et des dysfonctions dans le diabète de type 2 et des maladies associées
2020-2022
Université Lille Nord de France
2009-2012
Université de Rennes
2009-2012
Physique des interactions ioniques et moléculaires
2007
Enhancers are developmentally controlled transcriptional regulatory regions whose activities modulated through histone modifications or variant deposition. In this study, we show by genome-wide mapping that the newly discovered deoxyribonucleic acid (DNA) modification 5-hydroxymethylcytosine (5hmC) is dynamically associated with transcription factor binding to distal sites during neural differentiation of mouse P19 cells and adipocyte 3T3-L1 cells. Functional annotation reveals gaining 5hmC...
CCCTC-binding factor (CTCF) is a ubiquitously expressed multifunctional transcription characterized by chromatin binding patterns often described as largely invariant. In this context, how CTCF recruitment and functionalities are used to promote cell type-specific gene expression remains poorly defined. Here, we show that, in addition constitutively bound sites (CTS), the cistrome comprises large proportion of showing highly dynamic during course adipogenesis. Interestingly, positively...
Obesity is characterized by the excessive accumulation of dysfunctional white adipose tissue (WAT), leading to a strong perturbation metabolic regulations. However, molecular events underlying this process are not fully understood.MicroRNAs (miRNAs) small noncoding RNAs acting as posttranscriptional regulators gene expression in multiple tissues and organs. their roles WAT cell subtypes, which include only adipocytes but also immune, endothelial, mesenchymal stem cells well preadipocytes,...
Genome-wide association studies have reported that DNA polymorphisms at the CDKN2A locus modulate fasting glucose in human and contribute to type 2 diabetes (T2D) risk. Yet causal relationship between this gene defective energy homeostasis remains elusive. Here we sought understand contribution of Cdkn2a metabolic homeostasis. We first analyzed from Cdkn2a-deficient mice subjected normal or high fat diets. Subsequently primary adipose cells human-induced pluripotent stem differentiated into...
Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there need for thorough understanding the transcriptional regulatory involved pathophysiology. Recent advances functional annotation genome has highlighted role non-coding RNAs cellular processes coordination with factors. Using an unbiased genome-wide approach, we identified...
According to the Developmental Origin of Health and Disease (DOHaD) concept, maternal obesity accelerated growth in neonates predispose offspring white adipose tissue (WAT) accumulation. In rodents, adipogenesis mainly develops during lactation. The mechanisms underlying phenomenon known as developmental programming remain elusive. We previously reported that adult rat from high-fat diet-fed dams (called HF) exhibited hypertrophic adipocyte, hyperleptinemia increased leptin mRNA levels a...
Type 2 diabetes is characterized by chronic hyperglycemia associated with impaired insulin action and secretion. Although the heritability of type high, environment, including blood components, could play a major role in development disease. Amongst environmental effects, epitranscriptomic modifications have been recently shown to affect gene expression glucose homeostasis. The epitranscriptome reversible chemical changes RNA, one most prevalent being m6A methylation RNA. Since pancreatic β...
The nuclear receptor peroxisome proliferator-activated gamma (PPAR γ ) is required for differentiation and function of mature adipocytes. Its expression induced during adipogenesis where it plays a key role in establishing the transcriptome terminally differentiated white fat cells. Here, we review findings indicating that PPAR activity are intricately regulated through control chromatin structure. Hierarchical combinatorial activation transcription factors, noncoding RNAs, remodelers allows...
Only one drug is currently available for the treatment and control of schistosomiasis increasing risk selecting strains schistosome that are resistant to praziquantel means development new drugs urgent. With this objective we have chosen target enzymes modifying histones in particular histone acetyltransferases deacetylases (HDAC). Inhibitors HDACs (HDACi) under intense study as potential anti-cancer act via induction cell cycle arrest and/or apoptosis. Schistosomes like other parasites can...
According to the Developmental Origin of Health and Disease (DOHaD) concept, maternal obesity accelerated growth in neonates program later life. White adipose tissue (WAT) has been focus developmental programming events, although underlying mechanisms remain elusive. In rodents, WAT development primarily occurs during lactation. We previously reported that adult rat offspring from dams fed a high-fat (HF) diet exhibited fat accumulation decreased peroxisome proliferator-activated receptor γ...
Inhibition of histone deacetylases (HDACs) leads to growth arrest, differentiation, or apoptosis tumor cell lines, suggesting HDACs as promising targets for cancer therapy. At present, only one HDAC inhibitor (HDACi) is used in therapy: suberoylanilide hydroxamic acid (SAHA). Here, we describe the synthesis and biological evaluation a new series compounds derived from SAHA by substituting short alkyl chains at various positions phenyl ring. Such modifications induced variable effects ranging...
Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII/NR2F2) is an orphan member of the nuclear receptor family transcription factors whose activities are modulated upon binding small molecules into hydrophobic ligand-binding pocket (LBP). Although LBP COUP-TFII filled with aromatic amino-acid side chains, alternative modes ligand could potentially lead to regulation receptor. Here, we screened a synthetic and natural compound library in yeast one-hybrid assay identified...
The glucagon-like peptide 1 (Glp-1) has emerged as a hormone with broad pharmacological potential in type 2 diabetes (T2D) treatment, notably by improving β cell functions. cell-cycle regulator and transcription factor E2f1 is involved glucose homeostasis modulating mass function. Here, we report that cell-specific genetic ablation of (E2f1β−/−) impairs associated decreased expression the Glp-1 receptor (Glp1r) E2f1β−/− pancreatic islets. Pharmacological inhibition E2F1 transcriptional...
Type 2 diabetes (T2D) is a metabolic disorder characterized by loss of pancreatic β-cell function, decreased insulin secretion and increased resistance, that affects more than 537 million people worldwide. Although several treatments are proposed to patients suffering from T2D, long-term control glycemia remains challenge. Therefore, identifying new potential drugs targets positively affect function crucial. Here, we developed an automated approach allow the identification compounds or genes...
The loss of pancreatic β-cell identity has emerged as an important feature type 2 diabetes development, but the molecular mechanisms are still elusive. Here, we explore cell-autonomous role cell-cycle regulator and transcription factor E2F1 in maintenance identity, insulin secretion, glucose homeostasis. We show that β-cell–specific E2f1 function mice triggers intolerance associated with defective altered endocrine cell mass, downregulation many genes, concomitant increase non–β-cell...
The human pregnane X receptor (PXR) is a ligand-regulated transcription factor belonging to the nuclear superfamily. PXR activated by large, structurally diverse, set of endogenous and xenobiotic compounds coordinates expression genes central metabolism excretion potentially harmful chemicals therapeutic drugs in humans. Walrycin A novel antibacterial compound targeting WalK/WalR two-component signal transduction system Gram (+) bacteria. Here, we report that, hepatoma cells, walrycin...