A5077619790- Ion Channels and Receptors
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Nanoplatforms for cancer theranostics
- Bioactive Compounds and Antitumor Agents
- Nitric Oxide and Endothelin Effects
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Photodynamic Therapy Research Studies
- Sulfur Compounds in Biology
- Cancer, Hypoxia, and Metabolism
- Ion Transport and Channel Regulation
- Angiogenesis and VEGF in Cancer
- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Protein Kinase Regulation and GTPase Signaling
- Heat shock proteins research
- Estrogen and related hormone effects
- Receptor Mechanisms and Signaling
- Eicosanoids and Hypertension Pharmacology
- Neuroinflammation and Neurodegeneration Mechanisms
- Prostate Cancer Treatment and Research
- Signaling Pathways in Disease
- Antioxidant Activity and Oxidative Stress
Accademia Albertina delle Belle Arti
2001-2025
Université de Lille
2016-2024
Inserm
2015-2024
University of Turin
2014-2024
Centre National de la Recherche Scientifique
2022
La Ligue Contre le Cancer
2015-2017
Onco Lille
2017
Centro de Investigacion Principe Felipe
2014-2017
Bentham Science Publishers (United Arab Emirates)
2012
Bentham Science Publishers (China)
2012
Endothelial cells (ECs) play a key role to maintain the functionality of blood vessels. Altered EC permeability causes severe impairment in vessel stability and is hallmark pathologies such as cancer thrombosis. Integrating label-free quantitative proteomics data into genome-wide metabolic modeling, we built up model that predicts fluxes ECs when cultured on tridimensional matrix organize vascular-like network. We discovered how fatty acid oxidation increases are assembled fully formed...
TRPM8 is a cold sensor that highly expressed in the prostate as well other non-temperature-sensing organs, and regulated by downstream receptor-activated signaling pathways. However, little known about intracellular proteins necessary for channel function. Here, we identify two previously unknown proteins, which have named "TRP channel-associated factors" (TCAFs), new partner demonstrate they are TCAF1 TCAF2 both bind to promote its trafficking cell surface. exert opposing effects on gating...
Basic fibroblast growth factor (bFGF) and its major receptor FGF receptor-1 (FGFR-1) play an important role in the development of cortex. The mechanisms underlying mitogenic bFGF/FGFR-1 signaling have not been elucidated. Intracellular Ca 2+ concentrations ([Ca ] i ) proliferating cortical neuroepithelial cells are markedly dependent on entry (Maric et al., 2000a). absence voltage-dependent channels, which emerge later, indicates that other membrane regulate [Ca during proliferation....
Developmental processes mediating the initiation of lineage commitment from self-renewing neural stem cells (NSCs) remain mostly unclear because persisting ambiguity in identifying true NSCs proliferative lineage-restricted progenitors (LRPs), which are directly or indirectly derived NSCs. Our multilineage immunohistochemical analyses early embryonic rat telencephalon at onset neurogenesis revealed clear dorsoventral gradients emergence two types neuronal (NPs) multilineage-negative...
Endothelial cell adhesion and migration are critical steps of the angiogenic process, whose dysfunction is associated with tumor growth metastasis. The TRPM8 channel has recently been proposed to play a protective role in prostate cancer by impairing motility. However, mechanisms which it could influence vascular behavior unknown. Here, we reveal novel non-channel function for that unexpectedly acts as Rap1 GTPase inhibitor, thereby inhibiting endothelial motility, independently pore...
New evidence indicates the involvement of protein degradation dysfunctions in neurodegeneration, innate immunity response and alcohol hepatotoxicity. We recently demonstrated that ethanol increases brain proinflammatory mediators causes damage by activating Toll-like receptor 4 (TLR4) signaling glia. However, it is uncertain if ubiquitin-proteasome autophagy-lysosome pathways are involved ethanol-induced whether TLR4 implicated proteolytic processes. Using cerebral cortex WT TLR4-knockout...
Purinergic signaling is involved in inflammation and cancer. Extracellular ATP accumulates tumor interstitium, reaching hundreds micromolar concentrations, but its functional role on vasculature endothelium unknown. Here we show that high doses (>20 μM) strongly inhibit migration of endothelial cells from human breast carcinoma (BTEC), not normal microvascular EC. Lower (1-10 μM result ineffective. The anti-migratory activity associated with cytoskeleton remodeling significantly prevented by...
Background: Transient receptor potential (TRP) channels control multiple processes involved in cancer progression by modulating cell proliferation, survival, invasion and intravasation, as well as, endothelial (EC) biology tumor angiogenesis. Nonetheless, a complete TRP expression signature vessels, including prostate (PCa), is still lacking. Methods: In the present study, we profiled qPCR of all human tumor-derived ECs (TECs) comparison with TECs from breast renal tumors. We further...
Abstract Growth factor–induced intracellular calcium signals in endothelial cells regulate cytosolic and nuclear events involved the angiogenic process. Among messengers released after proangiogenic stimulation, arachidonic acid (AA) plays a key role its effects are strictly related to homeostasis cell proliferation. Here, we studied AA-induced derived from human breast carcinomas (B-TEC). AA promotes B-TEC proliferation organization of vessel-like structures vitro. The effect is directly...
Hydrogen sulphide (H2S) is a recently discovered gasotransmitter that may regulate growing number of endothelial functions, including nitric oxide (NO) release, proliferation, adhesion and migration, which are the key steps angiogenesis. The mechanism whereby H2S impacts on physiology still unclear: however, aforementioned processes driven by an increase in intracellular Ca2+ concentration ([Ca2+]i). In present study, we exploited excised rat aorta to gain insights into regulation [Ca2+]i...
Endothelial progenitor cells (EPCs) are mobilized into circulation to replace damaged endothelial and recapitulate the vascular network of injured tissues. Intracellular Ca(2+) signals key EPC activation, but it is yet be elucidated whether they endowed with same blend -permeable channels expressed by mature cells. For instance, colony forming (ECFCs), only subset truly committed acquire a phenotype, lack canonical transient receptor potential 3, 5 6 (TRPC3, 6), which widely distributed in...
Mucus is a complex barrier for pharmacological treatments and overcoming it one of the major challenges faced during transmucosal drug delivery. To tackle this issue, novel class glycosylated nanoparticles, named "mucosomes," which are based on most important protein constituting mucus, mucin, introduced. Mucosomes designed to improve absorption residence time mucosal tissues. produced (150-300 nm), functionalized with glycans, loaded desired in single one-pot synthetic process and, method,...
Abstract There is growing evidence that ion channels are critically involved in cancer cell invasiveness and metastasis. However, the molecular mechanisms of signaling promoting behavior poorly understood complexity underlying remodeling during metastasis remains to be explored. Here, using a variety vitro vivo techniques, we show metastatic prostate cells acquire specific Na + /Ca 2+ signature required for persistent invasion. We identify leak channel, NALCN, which overexpressed cancer, as...
We have analyzed the expression and localization of bovine transient receptor potential‐C1 (bTRPC1) in aortic endothelial cells, its possible involvement store‐independent calcium influx induced by basic fibroblast growth factor (bFGF). RT‐PCR experiments confirmed existence two btrpc1 mRNA isoforms; conversely, btrpc3 gene was not transcribed. Anti‐TRPC1 antibody revealed presence protein membrane‐rich compartment only. Application anti‐TRPC1 during response to bFGF caused a partial but...