A5027621531- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Hepatitis C virus research
- Viral-associated cancers and disorders
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Extracellular vesicles in disease
- Immune Response and Inflammation
- Intraperitoneal and Appendiceal Malignancies
- Lymphoma Diagnosis and Treatment
- Chemokine receptors and signaling
- Animal Virus Infections Studies
- Cancer Immunotherapy and Biomarkers
- Liver Disease Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Hypoxia, and Metabolism
- Inflammatory Bowel Disease
- Psoriasis: Treatment and Pathogenesis
- Photoacoustic and Ultrasonic Imaging
- Liver Diseases and Immunity
- Pancreatic and Hepatic Oncology Research
- Viral gastroenteritis research and epidemiology
- CAR-T cell therapy research
Centre National de la Recherche Scientifique
2015-2025
Université de Lille
2014-2025
Inserm
2020-2025
Centre Hospitalier Universitaire de Lille
2023-2025
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2023-2024
Onco Lille
2014-2024
Institut Pasteur de Lille
2007-2020
Institut de Biologie de Lille
2010-2020
Université Lille Nord de France
2012-2019
OncoThAI
2019
Regulatory T cells (Treg) and tumor-exosomes are thought to play a role in preventing the rejection of malignant patients bearing nasopharyngeal carcinoma (NPC). Treg recruitment by exosomes derived from NPC cell lines (C15/C17-Exo), isolated patients' plasma (Patient-Exo), CCL20 were tested vitro using Boyden chamber assays vivo xenograft SCID mouse model (n = 5), both presence absence anti-CCL20 monoclonal antibodies (mAb). Impact these (NPC-Exo) on phenotype function was determined...
Background Liver transplantation is the treatment of end-stage liver diseases, including hepatitis C. Immunosuppression prevents graft rejection but seems to accelerate recurrence Regulatory T cells (Tregs) may be beneficial in tolerance deleterious recurrent We evaluated effects cyclosporine or tacrolimus, principal immunosuppressive drugs, on Treg proliferation and function. Methods Human Tregs were isolated from healthy donors cultured with cyclosporine, NIM811, a analog devoid...
Inflammatory bowel diseases (IBDs) are chronic inflammatory of the gastrointestinal tract, which clinically present as 1 2 disorders, Crohn's disease (CD) or ulcerative colitis (UC) (Rogler 2004). The immune dysregulation in intestine plays a critical role pathogenesis IBD, involving wide range molecules, including cytokines. aim this work was to study involvement T-helper 17 (Th17) subset by nitric oxide (NO) pathway Algerian patients with IBD. We investigated correlation between...
Colitis associated cancer (CAC) is the colorectal (CRC) subtype that with bowel disease such as ulcerative colitis (UC). The data on role of NF-κB signaling in development and progression CAC were derived from preclinical studies, whereas human are rare. aim this work was to study contribution pathway during UC CAC, well immunomodulatory effect all-trans retinoic acid (AtRA). We analyzed expression NOS2, TNF-α, TLR4, NF-κB, colonic mucosa. also studied NO/TNF-α modulation by LPS mucosa...
CCL18 is both a constitutively expressed and an inducible chemokine, whose role in the inflammatory reaction poorly known. The aim of this study was to evaluate whether has capacity attract human T cells with regulatory function (regulatory [Treg]). Results from chemotaxis assays performed on different types Treg showed that CD4(+)CD25(+)CD127(low) cells, but neither type 1 clones nor differentiated vitro anti-CD3/CD46 mAbs, were recruited by dose-dependent manner. CCL18-recruited memory...
Galectin-9 (gal-9) is a multifunctional β-galactoside-binding lectin, frequently released in the extracellular medium, where it acts as pleiotropic immune modulator. Despite its overall immunosuppressive effects, recent study has reported bimodal action of gal-9 on human resting blood T cells with apoptosis occurring majority them, followed by wave activation and expansion Th1 surviving population. Our knowledge signaling events triggered exogenous remains limited. One these cytosolic...
Summary Epstein–Barr Virus ( EBV ) is present in the neoplastic cells of around 20–30% patients with Hodgkin Lymphoma HL ). Although, an immunosuppressive environment currently described patients, little known concerning regulatory mechanism induced by proteins expression tumour cells. This study aimed to investigate association between Type 1 (Tr1) and tissue positivity patients. Transcriptomic analysis both ‐positive ‐negative tumours showed that infection increased gene Tr1‐related...
The purpose of this study was to assess the direct effect CCL18, a chemokine elevated in allergic diseases and induced by Th2 cytokines, on polarization human CD4(+) T cells. Purified cells from healthy subjects were pretreated or not with evaluated for cytokine production. CCL18-pretreated memory but naive exhibited an increased production IL-10 (12.3 ± 2.6 vs. 5.6 0.9 ng/ml medium) TGF-β1 IL-4, IFN-γ, IL-17 compared control Pretreatment highly purified CD4(+)CD25(-) CCL18 led their...
Nasopharyngeal carcinoma (NPC) is a rare cancer, with 120,334 cases worldwide in 2022, but it remains endemic Southeast Asia and North Africa. Early-stage NPC typically treated radiotherapy, often combined chemotherapy for advanced stages [1]. Despite 5-year survival rate of 70% to 90% locoregional disease, late-stage diagnosis or distant recurrence metastasis (R/M) result poor prognosis many patients, underscoring the urgent need novel therapeutic strategies [2-4]. The tumor...
Immune response failure during HCV infection has been associated with the activity of regulatory T cells. Hepatitis C-related cirrhosis is main reason for liver transplantation. However, 80% transplanted patients present an accelerated recurrence disease. This study assessed involvement T-cell subsets (CD4+CD25+ cells: 'Treg' and CD49b+CD18+ 'T regulatory-1' cells), in after transplantation, using transcriptomic analysis, ELISA assays on serum samples immunohistochemistry biopsies from...
Often discovered at an advanced stage, ovarian cancer progresses to peritoneal carcinoma, which corresponds the invasion of serosa by multiple tumor implants. The current treatment is based on combination chemotherapy and cytoreduction surgery. Despite progress standardization surgical techniques combined with effective chemotherapy, post-treatment recurrences affect more than 60% women in remission. Photodynamic therapy (PDT) has been particularly indicated for superficial lesions large...
To date, pancreatic adenocarcinoma (ADKP) is a devastating disease for which the incidence rate close to mortality rate. The survival has evolved only 2–5% in 45 years, highlighting failure of current therapies. Otherwise, use photodynamic therapy (PDT), based on an adapted photosensitizer (PS) already proved its worth and prompted growing interest field oncology. We have developed new (PS-FOL/PS2), protected by recently published patent (WO2019 016397-A1, 24 January 2019). This associated...
H-1 parvovirus (H-1 PV), a rodent autonomous oncolytic parvovirus, has emerged as novel class of promising anticancer agents, because its ability to selectively find and destroy malignant cells. However, probe PV multimodal antitumor potential one the major prerequisites is decipher direct interplay with human immune system, so prevent any risk impairment.Non activated peripheral blood mononuclear cells (PBMCs) are not sensitive cytotoxic effect. virus impairs both PBMC proliferation...
Abstract Nasopharyngeal carcinoma‐derived small extracellular vesicles (NPCSEVs) have an immunosuppressive impact on the tumour microenvironment. In this study, we investigated their influence generation of tolerogenic dendritic cells and potential involvement galectin‐9 (Gal9) they carry in process. We analysed phenotype properties NPCSEVs explored ability DCs exposed to (NPCSEV‐DCs) regulate T cell proliferation. To assess at pathophysiological level, performed real‐time fluorescent...