A5002067050- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- Viral-associated cancers and disorders
- Growth Hormone and Insulin-like Growth Factors
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Insect Resistance and Genetics
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Parvovirus B19 Infection Studies
- Peptidase Inhibition and Analysis
- Tracheal and airway disorders
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Endometriosis Research and Treatment
- Herpesvirus Infections and Treatments
- Radiopharmaceutical Chemistry and Applications
- HER2/EGFR in Cancer Research
- Dysphagia Assessment and Management
- Toxin Mechanisms and Immunotoxins
- Endometrial and Cervical Cancer Treatments
- Click Chemistry and Applications
Wuhan University
2021-2024
Renmin Hospital of Wuhan University
2021-2024
Chinese Academy of Engineering
2023
Fusion Academy
2023
People's Liberation Army No. 150 Hospital
2023
Chinese People's Liberation Army
2023
Sechenov University
2022
Weatherford College
2021
Chengdu Research Base of Giant Panda Breeding
2007-2021
Zhejiang Sci-Tech University
2017-2019
Three vectors derived from retrovirus, herpes simplex virus type 1 (HSV), and adenovirus were compared in cultured rat 9L gliosarcoma cells for gene transfer efficiency a brain tumor model histologic pattern distribution of foreign delivery, as well associated necrosis inflammation. At multiplicity infection 1, vitro (lacZ Escherichia coli) into was more efficient with either the replication-defective retrovirus vector or replication-conditional thymidine kinase (TK)-deficient HSV than...
Most malignant tumors of the central nervous system do not respond well to chemotherapy. The anticancer drug cyclophosphamide (CPA) is largely ineffective against these neoplasms as its conversion DNA-alkylating, cytotoxic metabolites restricted primarily liver and readily cross blood–brain barrier. Here, we show that brain tumor cells can be sensitized effects CPA, both in culture vivo, by introduction hepatic enzyme responsible for activation cytochrome P450 2B1. Stable transfection rat C6...
Galectin-9 (gal-9) is a multifunctional β-galactoside-binding lectin, frequently released in the extracellular medium, where it acts as pleiotropic immune modulator. Despite its overall immunosuppressive effects, recent study has reported bimodal action of gal-9 on human resting blood T cells with apoptosis occurring majority them, followed by wave activation and expansion Th1 surviving population. Our knowledge signaling events triggered exogenous remains limited. One these cytosolic...
Rhenium (I)-diselenother (Re-diselenoether) is a water soluble metal-based compound, combining one atom of rhenium and two atoms selenium. This compound has been reported to exhibit marked activities against several solid tumor cell lines. We now disclose an improved synthesis this complex. The Re-diselenoether showed potent inhibitory effect on MDA-MB231 division in vitro, which lasted when the complex was no longer present culture. induced remarkable reduction volume primitive breast...
Objective: To investigate the differential expression of microRNA (miRNA) in patients with endometrial cancer and its relationship prognosis survival. Method: We used The Cancer Genome Atlas (TCGA) database to analyze differentially expressed miRNAs tissues adjacent normal tissues. In addition, we successfully screened out key microRNAs build nomogram models for predicting performed survival analysis on as well. Result: identified 187 miRNAs, which includes 134 up-regulated 53 down-regulated...
Intratumoral grafting of genetically engineered cells that produce interleukin-4 (IL-4) has been shown to tumor regression as well prolong survival mice harboring intracerebral gliomas. We sought determine whether retroviral-mediated gene delivery into in situ resulted enhanced by IL-4. Two mouse fibroblast lines were obtained: they both secreted similar levels IL-4 but one produced a retrovirus vector bearing the (CRE-MFG-IL-4 cells), whereas other did not (NIH3T3-IL-4 cells). In mixed...
Abstract Bombyx mori is one of the key lepidopteran model species, and economically important for silk production proteinaceous drug expression. Baculovirus insect host are natural biological models studying host–pathogen interactions. The impact nucleopolyhedrovirus (BmNPV) infection on proteome acetylome ovarian (BmN) cells explored to facilitate a better understanding infection‐driven interactions between BmNPV in vitro. profiled through six‐plex Tandem mass tag (TMT) labeling‐based...
Bombyx mori has become an important model organism for many fundamental studies. nucleopolyhedrovirus (BmNPV) is a significant pathogen to mori, yet also efficient vector recombinant protein production. A previous study indicated that acetylation plays vital roles in several cellular processes of while global phosphorylation pattern upon BmNPV infection remains elusive. Employing tandem mass tag (TMT) labeling and affinity enrichment followed by high-resolution LC-MS/MS analysis intensive...
Antibody-dependent cellular cytotoxicity (ADCC) is an important antiviral effector mechanism. ADCC to the protein encoded by Epstein-Barr virus (EBV) BamHI A rightward open-reading frame-1 (BARF1) was studied transducing Raji-tk- cells with BARF1 gene using a retroviral expression vector. The transduced Raji expressed on cell surface, as determined flow cytometry. Sera from chronic and acute infectious mononucleosis nasopharyngeal carcinoma patients were found contain antibodies that react...
Abstract Background Galectin-9 is a mammalian lectin which possesses immunosuppressive properties. Excessive production of galectin-9 has been reported in two types human virus-associated diseases chronic hepatitis C and nasopharyngeal carcinoma associated to the Epstein-Barr virus. The objective this study was produce new monoclonal antibodies targeting order improve its detection clinical samples, especially on tissue sections analysed by immunohistochemistry. Methods Hybridomas were...