A5015625005- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Virus-based gene therapy research
- Entomopathogenic Microorganisms in Pest Control
- Plant Virus Research Studies
- Invertebrate Immune Response Mechanisms
- Animal Virus Infections Studies
- Neurobiology and Insect Physiology Research
- interferon and immune responses
- Silk-based biomaterials and applications
- Heat shock proteins research
- Protein Degradation and Inhibitors
- Immune Response and Inflammation
- Signaling Pathways in Disease
- Studies on Chitinases and Chitosanases
- CRISPR and Genetic Engineering
- Influenza Virus Research Studies
- thermodynamics and calorimetric analyses
- Aquaculture disease management and microbiota
- Viral Infections and Immunology Research
- Gastrointestinal Tumor Research and Treatment
- Advanced Battery Materials and Technologies
- Silkworms and Sericulture Research
- Atherosclerosis and Cardiovascular Diseases
- T-cell and Retrovirus Studies
Zhejiang Sci-Tech University
2016-2025
The University of Texas MD Anderson Cancer Center
2016
China National Silk Museum
2013
The Dainippon Silk Foundation
2008
University College Dublin
2008
Avian influenza virus (H5N1) has caused serious infections in human beings. This the potential to emerge as a pandemic threat humans. Effective vaccines against H5N1 are needed. A recombinant Bombyx mori baculovirus, Bmg64HA, was constructed for expression of HA protein displaying on viral envelope surface. The accounted approximately 3% total proteins silkworm pupae infected with virus. Using series separation and purification methods, pure Bmgp64HA isolated from these bioreactors. Aluminum...
Background: Porcine reproductive and respiratory syndrome is caused by PRRSV. Modified live vaccines (MLVs) are widely used to control PRRSV infection, but their efficacy against the emerging NADC30-like variant remains unclear. This study aimed evaluate of a VR-2332-based MLV strain HNjz15. Methods: Forty piglets were randomized into vaccination group (MLV group), negative (NC sentinel group. immunized with commercial at 3 weeks age challenged HNjz15 (106.6 TCID50/mL) 21 days...
Abstract Bombyx mori is one of the key lepidopteran model species, and economically important for silk production proteinaceous drug expression. Baculovirus insect host are natural biological models studying host–pathogen interactions. The impact nucleopolyhedrovirus (BmNPV) infection on proteome acetylome ovarian (BmN) cells explored to facilitate a better understanding infection‐driven interactions between BmNPV in vitro. profiled through six‐plex Tandem mass tag (TMT) labeling‐based...
Bombyx mori has become an important model organism for many fundamental studies. nucleopolyhedrovirus (BmNPV) is a significant pathogen to mori, yet also efficient vector recombinant protein production. A previous study indicated that acetylation plays vital roles in several cellular processes of while global phosphorylation pattern upon BmNPV infection remains elusive. Employing tandem mass tag (TMT) labeling and affinity enrichment followed by high-resolution LC-MS/MS analysis intensive...
Highlights•Deficiency of CARMA3 results in the host resistance to RNA viral infection•CARMA3 positively regulates RIG-I/MAVS-mediated NF-κB activation•CARMA3 negatively TBK1/IRF3 suppresses MAVS oligomerization mitochondranSummaryHost response virus infection is sensed by sensors such as RIG-I, which induces MAVS-mediated and IRF3 activation promote inflammatory antiviral responses, respectively. Here, we have found that CARMA3, a scaffold protein previously shown mediate induced GPCR EGFR,...
Although the 30K family proteins are important anti-apoptotic molecules in silkworm hemolymph, underlying mechanism remains to be investigated. This is especially case human vascular endothelial cells (HUVECs). In this study, a protein, 30Kc6, was successfully expressed and purified using Bac-to-Bac baculovirus expression system cells. Furthermore, 30Kc6 Escherichia coli used generate polyclonal antibody. Western blot analysis revealed that antibody could react specifically with The vitro...
Silkworm ( Bombyx mori ) is a model organism with great agricultural economic value that plays crucial role in biological studies. B. nucleopolyhedrovirus (BmNPV) major viral pathogen found silkworms, which leads to huge silk loss annually. In recent lysine acetylome of silkworm infected BmNPV, we focused on the heat shock cognate protein 70-4 (HSC70-4) acetylation change due consequent nuclear accumulation and structure assembly. this study, genome replication, proliferation, production...
Abstract HSP90 is a highly conserved chaperone that facilitates the proliferation of many viruses, including silkworm ( bombyx mori ) nucleopolyhedrovirus (BmNPV), but underlying regulatory mechanism was unclear. We found suppression by 17‐AAG, HSP90‐specific inhibitor, significantly reduced expression BmNPV capsid protein gp64 and viral genome replication, whereas overexpression B. HSP90(BmHSP90) promoted replication. Furthermore, in recent study lysine acetylome infected with BmNPV, we...
Storage protein 2 (SP2) not only is an important source of energy for the growth and development silkworm but also has inhibitory effects on cell apoptosis. Endothelial (EC) apoptosis contributing factor in atherosclerosis; therefore, study antiapoptotic activity SP2 ECs provides information related to treatment atherosclerosis other cardiovascular diseases. In this study, sp2 gene was cloned expressed Escherichia coli produce a 6xHis-tagged fusion protein, which then used generate...
The 30K family of proteins is important in energy metabolism and may play a role inhibiting cellular apoptosis silkworms ( Bombyx mori ). Several 30K-family have been identified. In this study, two new silkworm genes, referred to as Slp (NM 001126256) Lsp-t 001043443), were analyzed by bioinformatics approach according the sequences previously reported silkworm. Both shared more than 41% amino acid sequence homology with displayed conserved domain consistent that lipoprotein-11....
A DNA-binding protein (DBP) [GenBank accession number: M63416] of Bombyx mori nuclear polyhedrosis virus (BmNPV) has been reported to be a regulatory factor in BmNPV, but its detailed functions remain unknown. In order study the mechanism DBP on viral proliferation, genome replication, and gene transcription, BmNPV dbp knockout dbp-ko-Bacmid was generated by means Red recombination system. addition, dbp-repaired dbp-re-Bacmid constructed Bac Then, Bacmids were transfected into BmN cells. The...
Hematological and neurological expressed 1-like (HN1L) protein is an evolutionarily conserved that plays important role in embryonic development. It has been reported HN1L involved the process of cell growth cancer formation cycle arrest occurs during suppression expression. Previous studies have demonstrated expression levels Bombyx mori were significantly downregulated Nucleopolyhedrovirus (BmNPV) infected silkworm cells. Transient transfections performed with plasmids for pIEX-1-HN1L...
Bombyx mori nucleopolyhedrovirus (BmNPV) is a specific pathogen of that can significantly impede agricultural development. Accumulating evidence indicates the viral proliferation in host requires an ample supply energy. However, correlative reports baculovirus are deficient, especially on acetylation modification tricarboxylic acid cycle (TCA cycle) metabolic enzymes. Our recent quantitative analysis protein acetylome revealed mitochondrial aconitase (ACO2) could be modified by...
Abstract We have developed a novel baculovirus surface display (BVSD) system for the isolation of membrane proteins. expressed reporter gene that encoded hemagglutinin fused in frame with signal peptide and transmembrane domain gp64 protein, which is displayed on BmNPV virions. The expression this fusion protein virion envelope allowed us to develop two methods isolating In first method, we isolated proteins directly from budding second cellular membranes had disintegrated due viral egress....