A5103198420- Viral Infectious Diseases and Gene Expression in Insects
- Silk-based biomaterials and applications
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Protein Hydrolysis and Bioactive Peptides
- Peptidase Inhibition and Analysis
- Neurobiology and Insect Physiology Research
- Studies on Chitinases and Chitosanases
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Histone Deacetylase Inhibitors Research
- Antimicrobial Peptides and Activities
- Signaling Pathways in Disease
- Circular RNAs in diseases
- Polysaccharides Composition and Applications
- Biological and pharmacological studies of plants
- Heat shock proteins research
- Silkworms and Sericulture Research
- Gastrointestinal Tumor Research and Treatment
- Chromosomal and Genetic Variations
- Bioactive natural compounds
- Beetle Biology and Toxicology Studies
Zhejiang Sci-Tech University
2015-2025
Shaoxing University
2024
Tsinghua University
2018
Central South University
2001-2009
The Dainippon Silk Foundation
2008
Alzheimer’s disease (AD) is currently the most common neurodegenerative disease. Glycogen synthase kinase 3β (GSK-3β) a pivotal factor in AD pathogenesis. Recent research has demonstrated that plant miRNAs exert cross-kingdom regulation on target genes animals. Gastrodia elata (G. elata) valuable traditional Chinese medicine significant pharmacological activity against diseases of central nervous system (CNS). Our previous studies have indicated G. elata-specific miRNA plays regulatory role...
Abstract Background MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression by targeting messenger RNAs (mRNAs) and causing mRNA cleavage or translation blockage. Of the 355 Arthropod miRNAs have been identified, only 21 B. mori were predicted computationally; of these, let-7 has confirmed Northern blotting. Results Combining a computational method based on sequence homology searches with experimental identification microarray assays blotting, we identified 46 miRNAs, an...
Abstract Background Small non-coding RNAs (ncRNAs) are important regulators of gene expression in eukaryotes. Previously, only microRNAs (miRNAs) and piRNAs have been identified the silkworm, Bombyx mori . Furthermore, ncRNAs (50-500nt) intermediate size systematically silkworm. Results Here, we performed a systematic identification analysis small (18-50nt) associated with argonaute2 (BmAgo2) protein. Using RIP-seq, various types BmAGO2. These showed multimodal length distribution, three...
Lysine acetylation in proteins is a dynamic and reversible PTM plays an important role diverse cellular processes. In this study, using lysine-acetylation (Kac) peptide enrichment coupled with nano HPLC/MS/MS, we initially identified the acetylome silkworms. Overall, total of 342 acetylated 667 Kac sites were silkworm. Sequence motifs analysis around revealed Y, F, H +1 position, F was also enriched +2 -2 positions, indicating presences preferred amino acids Functional showed primarily...
As a key acetyltransferase, Bombyx mori cAMP response element-binding protein (CREB)-binding (BmCBP) plays an important role in post-translational modifications, affecting stability and immune silkworm; however, its mechanism of action remains unclear. In this study, we designed synthesized Nano-dsRNA nanoparticles targeting BmCBP validated their impact on BmApoLp-III expression. Using E. coli expression system, successfully nano-dsRNA BmCBP, which significantly reduced transcription level,...
Uric acid is a key intermediate in purine degradation across diverse organisms, while its accumulation humans leads to inflammation and gout disease. Aerobic organisms degrade uric via well-known "oxidative pathway" involving dearomatization of the core catalyzed by oxidases or dehydrogenases. The ability also widespread anaerobic bacteria, including gut although mechanisms are incompletely understood. Here, we report biochemical characterization recently identified gene cluster from...
Abstract Background The completion and reporting of baculovirus genomes is extremely important as it advances our understanding gene function evolution. Due to the large number viral now sequenced very that authors present significantly detailed analyses advance genomes. However, there no report Antheraea pernyi nucleopolyhedrovirus (AnpeNPV) genome. Results genome AnpeNPV, which infects Chinese tussah silkworm ( ), was analyzed. 126,629 bp in size. G+C content genome, 53.4%, higher than...
To determine whether cholera toxin B subunit and active peptide from shark liver (CTB-APSL) fusion protein plays a role in treatment of type 2 diabetic mice, the CTB-APSL gene was cloned expressed silkworm (Bombyx mori) baculovirus expression vector system (BEVS), then orally administrated at dose 100 mg/kg for five weeks mice. The results demonstrated that oral administration can effectively reduce levels both fasting blood glucose (FBG) glycosylated hemoglobin (GHb), promote insulin...
On the basis of a class 2D graphical representations DNA sequences, sensitivity analysis has been performed, showing high-capability proposed to take into account small modifications sequences. And also indicates that absolute differences leading eigenvalues L/L matrices associated with increase number base mutations. Besides, we conclude similarity method based on correlation angles can better eliminate effects lengths sequences if compared using Euclidean distances. As application,...
Oncogenic KIT or PDGFRA receptor tyrosine kinase (TK) mutations are compelling therapeutic targets in gastrointestinal stromal tumors (GISTs), and the KIT/PDGFRA inhibitor, imatinib, is standard of care for patients with metastatic GIST. However, approximately 10% KIT-positive GIST metastases lose expression at time clinical progression during imatinib therapy. In present report, we performed TK-activation screens, using phosphotyrosine-TK double immunoaffinity purification mass...
Gastrodia elata Blume (G. elata) is a valuable traditional Chinese medicine with neuroprotection, anti-inflammatory, and immune regulatory functions. MicroRNAs (miRNA) kind of endogenous noncoding small RNAs that plays distinctly important roles for gene regulation organisms. So far, the research on G. mainly focused pharmacological functions natural chemical ingredients, function miRNA remains unknown. In this study, 5,718 known miRNAs 38 novel were identified by high-throughput sequencing...
This study aimed to investigate the effect of nitrogen sources on production and rheological properties welan gum produced by <i>Sphingomonas</i> sp. ATCC 31555. Six different were used for 31555 fermentation, 2 these further analyzed due their more positive influence bacterial biomass. Bacterial biomass, yield, viscosity, molecular weight, monosaccharide composition, acyl content, structure analyzed. Welan biomass concentration higher in media containing...
To understand the mechanisms of liver regeneration better to promote research examining diseases and marine biology, normal regenerative tissues Chiloscyllium plagiosum were harvested 0 h 24 after partial hepatectomy (PH) used isolate small RNAs for miRNA sequencing. In total, 91 known miRNAs 166 putative candidate (PC) identified first time in plagiosum. Through target prediction GO analysis, 46 screened specially cellular proliferation growth. Differential expression levels three...
Active peptide from shark liver (APSL) is a cytokine Chiloscyllium plagiosum that can stimulate regeneration and protects the pancreas. To study effect of orally administered recombinant APSL (rAPSL) on an animal model type 2 diabetes mellitus, gene was cloned, expressed in Bombyx mori N cells (BmN cells), silkworm larvae pupae using baculovirus expression vector system (BEVS). It demonstrated rAPSL able to significantly reduce blood glucose level mice with induced by streptozotocin. The...
Abstract Acetylation is an important, highly conserved, and reversible post‐translational modification of proteins. Previously, we showed by nano‐HPLC/MS/MS that many nutrient storage proteins in the silkworm are acetylated. Among these proteins, most known 30K were shown to be acetylated, including 23 acetylated containing 49 sites (Kac), indicating importance acetylation silkworm. In this study, Bm30K‐3, a protein three Kac sites, was further assessed functional studies its acetylation....