A5033570632- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related gene regulation
- HER2/EGFR in Cancer Research
- Colorectal Cancer Treatments and Studies
- Chronic Myeloid Leukemia Treatments
- Genomics and Chromatin Dynamics
- Pancreatitis Pathology and Treatment
- Pancreatic function and diabetes
- Pancreatic and Hepatic Oncology Research
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Rheumatoid Arthritis Research and Therapies
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Drug Transport and Resistance Mechanisms
- Extracellular vesicles in disease
- Liver physiology and pathology
- S100 Proteins and Annexins
- Thyroid Cancer Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Ferroptosis and cancer prognosis
de Duve Institute
2015-2025
UCLouvain
2014-2025
Cliniques Universitaires Saint-Luc
2014
KU Leuven
2009-2010
Ludwig Cancer Research
2003-2007
Zero to Three
2007
Several male germ line-specific genes, including MAGE-A1, rely on DNA methylation for their repression in normal somatic tissues. These genes become activated many types of tumors the course genome-wide demethylation process which often accompanies tumorigenesis. We show that tumor cells expressing 5' region is significantly less methylated than other parts gene. The leading to this site-specific hypomethylation does not appear be permanent these cells, since vitro-methylated MAGE-A1...
Genome-wide loss of DNA methylation is commonly observed in human cancers, but its impact on the tumor transcriptome remains ill-defined. Previous studies demonstrated that this epigenetic alteration causes aberrant activation a germline-specific gene expression program. Here, we examined if hypomethylation tumors also leads to de-repression clusters with other tissue specificities. To end, explored transcriptomic and methylomic datasets from lung adenocarcinoma (LUAD) cell lines, normal...
Abstract Background Following solid organ transplantation, tacrolimus (TAC) is an essential drug in the immunosuppressive strategy. Its use constitutes a challenge due to its narrow therapeutic index and high inter- intra-pharmacokinetic (PK) variability. As contribution of gut microbiota metabolism now emerging, it might be explored as one factors explaining TAC PK Herein, we consequences administration on composition. Reciprocally, studied PK, using combination vivo vitro models. Results...
Genome hypomethylation is a common epigenetic alteration in human tumors, where it often leads to aberrant activation of group germline-specific genes, commonly referred as "cancer-germline" genes. The cellular functions and tumor promoting potential these genes remain, however, largely uncertain. Here, we report identification novel cancer-germline transcript (CT-GABRA3) displaying DNA hypomethylation-dependent various including melanoma lung carcinoma. Importantly, CT-GABRA3 harbors...
MAGE-A1 belongs to a group of germ line-specific genes that rely primarily on DNA methylation for repression in somatic tissues. In many types tumors, the promoter these becomes demethylated and transcription activated. We showed previously that, although MZ2-MEL melanoma cells contain an active unmethylated gene, they lack ability induce demethylation newly integrated transgenes were methylated vitro before transfection. same cells, protected against remethylation, this appeared depend...
Preeclampsia is one of the leading causes maternal mortality worldwide and strongly associated with long-term morbidity in mothers newborns. Referred to as deep placentation disorders, insufficient remodeling spiral arteries during first trimester remains a major cause placental dysfunction. Persisting pulsatile uterine blood flow abnormal ischemia/reoxygenation phenomenon placenta stabilizes HIF-2α (hypoxia-inducible factor-2α) cytotrophoblasts. signaling impairs trophoblast differentiation...
Alterations in bile acid profile and pathways contribute to hepatic inflammation cancer cachexia, a syndrome worsening the prognosis of patients. As gut microbiota impinges on host metabolism through acids, current study aimed explore functional contribution microbial dysbiosis dysmetabolism associated disorders cachexia. Using three mouse models cachexia (the C26, MC38 HCT116 models), we evidenced reduction levels several secondary mainly taurodeoxycholic (TDCA). This TDCA occurred before...
Afatinib-induced tumor and microenvironment modifications in head neck squamous cell carcinoma were evaluated by spatial transcriptomics surgical specimens RNA-sequencing biopsies of patients included the EORTC-90111-24111 window-of-opportunity study. The aim was to explore evolution composition under anti-HER therapy. Based on our previous investigations RNA-seq biopsies, slides ID08 ID15 from epithelial-to-mesenchymal (EMT) cluster ID30 non-EMT investigated with transcriptomics. Dimension...
Stimulator of interferon genes (STING) is a pivotal mediator anti-tumor immunity, activated downstream cytoplasmic DNA recognition by cyclic GMP-AMP synthase (cGAS). In cells that employ the alternative lengthening telomeres (ALT) pathway, extrachromosomal telomeric repeats (ECTRs) act as potent activators cGAS-STING pathway. Although ALT+ were previously thought to evade this pathway through epigenetic silencing, our findings reveal more nuanced adaptive mechanisms involve spatial...
Abstract An important class of tumor‐specific antigens is encoded by male germline‐specific genes, such as MAGE that are activated in many cancers various histological types a result the demethylation their promoter region. A number these genes were shown to be expressed exclusively during spermatogonia stage spermatogenesis. recent study reported isolation new set mouse but not somatic tissues. Here, we tested tumoral expression human orthologs 12 genes. remarkably high proportion, i.e. , 5...
Several genes with specific expression in germ cells show aberrant activation different types of tumors. These genes, termed cancer-germline (CG) encode tumor-specific antigens, which represent potential targets for therapeutic vaccination against cancer. The germline-specific gene BORIS (Brother Of the Regulator Imprinted Sites), encodes an 11-zinc-fingers transcriptional regulator, was recently qualified as a new CG gene, it found to be activated variety tumor samples. Moreover, suggested...
Transcriptomic profiling of synovial tissue from patients with early, untreated rheumatoid arthritis (RA) was used to explore the ability unbiased, data-driven approaches define clinically relevant subgroups.RNASeq performed on 74 samples, disease activity data collected at inclusion. Principal components analysis (PCA) and unsupervised clustering were patient clusters based expression most variable genes, followed by pathway inference relative abundance immune cell subsets. Histological...
Gene MAGEA1 belongs to a group of human germline-specific genes that rely on DNA methylation for repression in somatic tissues. Many these genes, termed cancer-germline (CG) become demethylated and activated wide variety tumors, where they encode tumor-specific antigens. The process leading demethylation CG tumors remains unclear. Previous data suggested histone acetylation might be involved. Here, we investigated the relative contribution epigenetic regulation gene MAGEA1. We show...
Aging | doi:10.18632/aging.204700. Giulia Jannone, Eliano Bonaccorsi Riani, Catherine de Magnée, Roberto Tambucci, Jonathan Evraerts, Joachim Ravau, Pamela Baldin, Caroline Bouzin, Axelle Loriot, Laurent Gatto, Anabelle Decottignies, Mustapha Najimi, Etienne Marc Sokal
Epigenetic dysfunctions, including DNA methylation alterations, play major roles in cancer initiation and progression. Although it is well established that gene promoter demethylation activates transcription, remains unclear whether hypomethylation of repetitive heterochromatin similarly affects expression non-coding RNA from these loci. Understanding how RNAs are transcriptionally regulated important given their upregulation by the heat shock (HS) pathway suggests functions cellular...
Telomeric repeat-containing RNA (TERRA) molecules play important roles at telomeres, from heterochromatin regulation to telomerase activity control. In human cells, TERRA is transcribed subtelomeric promoters located on most chromosome ends and associates with telomeres. The origin of mouse is, however, unclear, as transcription the pseudoautosomal PAR locus was recently suggested account for vast majority in embryonic stem cells (ESC). Here, we confirm production both 18q telomere...
Abstract Pancreatic acinar cells are a cell type of origin for pancreatic cancer that become progressively less sensitive to tumorigenesis induced by oncogenic Kras mutations after birth. This sensitivity is increased when combined with pancreatitis. Molecular mechanisms underlying these observations still largely unknown. To identify mechanisms, we generated the first CRISPR-edited mouse models enable detection wild-type and mutant KRAS proteins in vivo. Analysis revealed more than 75%...
Biliary ducts collect bile from liver lobules, the smallest functional and anatomical units of liver, carry it to gallbladder. Disruptions in this process caused by defective embryonic development, or through ductal reaction disease have a major impact on life quality survival patients. A deep understanding processes underlying duct lumen formation is crucial identify intervention points avoid treat appearance ducts. Several hypotheses been proposed characterize biophysical mechanisms...
Intestinal T cells are key in gut barrier function. Their role early stages of alcohol-associated liver disease (ALD) remain unknown.To explore the links between intestinal cells, microbial translocation and ALD METHODS: Patients with alcohol use disorder (AUD) following a rehabilitation programme were compared to subjects non-alcoholic fatty (NAFLD) healthy controls. Clinical laboratory data (liver stiffness, controlled attenuation parameter, AST, ALT, K18-M65) served identify AUD patients...