A5061045642- Glycosylation and Glycoproteins Research
- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- Carbohydrate Chemistry and Synthesis
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Galectins and Cancer Biology
- Gastric Cancer Management and Outcomes
- Pancreatic and Hepatic Oncology Research
- Healthcare Systems and Practices
- Bariatric Surgery and Outcomes
- Workplace Health and Well-being
- Occupational Health and Safety Research
- Cancer Cells and Metastasis
- Employment and Welfare Studies
- Health, Medicine and Society
- COVID-19 and healthcare impacts
- Peptidase Inhibition and Analysis
- GDF15 and Related Biomarkers
- COVID-19 Clinical Research Studies
- Inflammatory Bowel Disease
- Health Promotion and Cardiovascular Prevention
- Pharmaceutical Practices and Patient Outcomes
- Proteoglycans and glycosaminoglycans research
- Chronic Disease Management Strategies
l'Assurance Maladie
2016-2024
Conservatoire National des Arts et Métiers
2019-2020
Inserm
2004-2016
Sorbonne Université
2002-2014
Institut Pierre Louis d‘Épidémiologie et de Santé Publique
2014
Centre de Recherche Saint-Antoine
2010-2013
Université Paris Cité
2006-2010
Centre de Recherche Jean Pierre Aubert
2010
Institut Pasteur de Lille
2010
Hôpital Saint-Antoine
2010
ABSTRACT Mucin expression was analysed, in relation to cell growth, parental HT-29 cells and two populations of mucus-secreting selected by adaptation methotrexate (HT29-MTX) or 5-fluorouracil (HT29-FU). These express mature mucins that differ their immunoreactivity antibodies against gastric colonic In the population, at late confluency, only very few produce MUC1 glycoprotein, this being consistent with low level mRNAs corresponding MUC5C mucin genes. HT29-MTX HT29-FU populations,...
Abstract Defects in apoptosis have been implicated chemoresistance of colon cancer cells. We report here the ability to resist 5‐fluorouracil‐induced 8 cell lines differing p53 and bax status: −/0 +/+ for TC7, SW480, HT‐29; −/− LS174T, LoVo; +/− HCT116; or +/0 LS513 HCT‐EB, respectively. To approximate vivo therapy, were exposed a long‐term treatment 5‐FU. The analysis proteins apoptotic pathway has shown that independent status was not sufficient predict extent drug‐resistance all lines. In...
AbstractThe homeostatic self-renewal of the colonic epithelium requires coordinated regulation canonical Wnt/β-catenin and Notch signaling pathways to control proliferation lineage commitment multipotent stem cells. However, molecular mechanisms by which Notch1 interplay in controlling cell fate colon are poorly understood. Here we show that NADPH oxidase 1 (NOX1), a reactive oxygen species (ROS)-producing is highly expressed epithelial cells, pivotal determinant integrates signals....
Abstract Background Lifelong medical follow-up is mandatory after bariatric surgery. The aim of this study was to assess the 5-year surgery in a nationwide cohort patients. Methods All adult obese patients who had undergone primary 2009 France were included. Data extracted from French national health insurance database. Medical (medical visits, micronutrient supplementation and blood tests) during first 5 years assessed, compared with international guidelines. Results Some 16 620 included...
CARD15/NOD2 mutations are associated with susceptibility to Crohn's Disease (CD) and Graft Versus Host (GVHD). CD GVHD suspected be related the dysfunction of Peyer's patches (PP) isolated lymphoid follicles (LFs). Using a new mouse model invalidated for Card15/Nod2 (KO), we thus analysed impact gene in these formations together development experimental colitis.At weeks 4, 12 52, numbers PPs LFs were higher KO mice while no difference was observed at birth. At 4 12, size cellular composition...
Abstract Metastasis and drug resistance are major problems in cancer chemotherapy. The purpose of this work was to analyze the molecular mechanisms underlying invasive potential drug-resistant colon carcinoma cells. Cellular models included parental HT-29 cell line its derivatives selected after chronic treatment with either 5-fluorouracil, methotrexate, doxorubicin, or oxaliplatin. Drug-resistant cells were compared noninvasive using cDNA microarray, quantitative reverse transcription-PCR,...
Nucleotide oligomerisation domain 2 ( NOD2 ) mutations are associated with susceptibility to Crohn's disease and graft-versus-host disease, two human disorders related dysfunctions of Peyer's patches (PPs). In Nod2 −/− mice transcellular permeability bacterial translocation increased in PPs. this study, we show that both anti-CD4 + anti-interferon γ (anti-IFN monoclonal antibodies abrogate phenotype reduce the expression tumour necrosis factor (TNF) receptor long isoform myosin light chain...
A better understanding of the mechanisms by which colon tumor cells are able to survive exposure drugs would be valuable for development new therapeutic strategies. We used differential display-PCR compare gene expression in drug-sensitive HT-29 cancer cell line and 3 drug-resistant subpopulations derived from this parental line. One genes identified is a gene, Regenerating IV (Reg IV), was strongly overexpressed cells. Other lines expressed Reg at high level, whereas low noted sensitive...
Postconfluent cultures of HT-29 cells form a heterogeneous multilayer which greater than 95% the are undifferentiated. In contrast, when stably adapted to normally lethal concentrations methotrexate (10(-6)-10(-5) M), they monolayer gobletlike (Lesuffleur et al., 1990) secrete large quantities mucins and display discrete brush border with presence villin, dipeptidylpeptidase-IV, carcinoembryonic antigen. When even higher (10(-4) 10(-3) M) there is shift in pattern differentiation from...
Exposure for 24 h of mucus-secreting HT-29 cells to the sugar analogue GalNAc-α-O-benzyl results in inhibition Galβ1-3GalNAc:α2,3-sialyltransferase, reduced mucin sialylation, and their secretion (Huet, G., I. Kim, C. de Bolos, J.M. Loguidice, O. Moreau, B. Hémon, Richet, P. Delannoy, F.X. Real., Degand. 1995. J. Cell Sci. 108:1275–1285). To determine effects prolonged differentiated populations were grown under permanent exposure GalNAc-α-O-benzyl. This not only mucus secretion, but also a...
HT-29 cells resistant to 10−6M methotrexate (HT29-MTX) secrete mucins with gastric immunoreactivity (Lesuffleur, T., Barbat, A., Dussaulx, E., and Zweibaum, A.(1990) Cancer Res. 50, 6334-6343). A 3310-base pair mucin cDNA clone (L31) was isolated from an HT29-MTX expression library using a polyclonal serum specific for normal mucosa. It shows high level of identity (98.6%) NP3a nasal polyp (Meerzaman, D., Charles, P., Daskal, Polymeropoulos, M. H., Martin, B. M., Rose, C.(1994) J. Biol....
Abstract The HT‐29 cell line contains a small proportion of differentiated polarized, enterocytic and mucus‐secreting types (< 95%) which can be selected under various pressure conditions, e.g., glucose deprivation or methotrexate. purpose the present work was to investigate whether this also applies 5‐fluorouracil (FUra). Stepwise adaptation exponentially growing cells 1, 5, 10 20 μM FUra results, after phase high mortality, in emergence adapted sub‐populations with stable growth rates...
A current challenge is to define the biological characteristics of colon tumor cells resistant chemotherapy. Distinct sub-populations mucus-secreting were previously obtained from cancer cell line HT-29 after long-term treatment with anti-cancer drugs, 5-fluorouracil (5-FU) and methotrexate (MTX). Since mucins are increasingly implicated as playing a role in carcinogenesis, we studied pattern mucin expression two clones enterocyte-like phenotype which differ their capacity resist 5-FU and/or...
Microsatellite instability (MSI) due to mismatch repair (MMR) deficiency is reported in 5–10% of colorectal cancers (CRCs) complicating inflammatory bowel diseases (IBD). The molecular mechanisms underlying MMR may be different IBD CRCs, and sporadic hereditary MSI tumors. Here, we hypothesize that overexpression miR-155 miR-21, two inflammation-related microRNAs target core proteins, constitute a pre-neoplastic event for the development CRCs. We studied miR-21 expression using real-time...
ABSTRACT Neoplastic transformation is commonly associated with altered glycosylation of proteins and lipids. To understand the basis for mucin glycosylation, we have examined distribution RER markers, a cis-Golgi resident protein, GalNAc α-O-Ser/Thr epitope (Tn) in human colon cancer cells normal colon. In cultured mucin-producing cells, Gal-NAc was found droplets cisternae. addition, Golgi apparatus disorganized proportion 130 kDa protein also abnormally redistributed to RER. The MUC2...
Adaptation of the heterogeneous human colon carcinoma cell line HT-29 to lethal concentrations methotrexate (MTX) and 5-fluorouracil (FUra) was shown result in emergence sub-populations cells all stably committed differentiation. It postulated that these populations from selection a few present parental which possess, associated with their ability differentiate, particular advantages allowing them adapt adverse conditions such as MTX or FUra. The purpose study further verify this hypothesis...
Nucleotide oligomerisation domain 2 (NOD2) is a component of the innate immunity known to be involved in homeostasis Peyer patches (PPs) mice. However, little about its role during gut infection vivo. Yersinia pseudotuberculosis an enteropathogen causing gastroenteritis, adenolymphitis and septicaemia which able invade host through PPs. We investigated Nod2 Y. infection. Death was delayed deleted Crohn's disease associated mutated mice orogastrically inoculated with pseudotuberculosis. In...
REG4, the latest member of regenerating gene family, is overexpressed in inflammatory bowel diseases and gastrointestinal carcinomas. To date, its pathophysiologic role has not been well established. Using HT-29 models, we previously identified REG4 as being colorectal tumor cells displaying a drug-resistance phenotype; some also displayed invasive properties. Thus, investigated potential functions biological processes involved progression such cell proliferation, migration invasion. Colon...
Little is known about associations between emergent psychosocial work factors and mental health. To explore classical depression anxiety symptoms in employees France. A national cross-sectional study (the SUrveillance Médicale des Expositions aux Risques professionnels (SUMER) survey) assessed including psychological demands, decision latitude, social support, reward its sub-dimensions (esteem, job security promotion), bullying, verbal abuse, physical violence sexual assault, long working...
Mismatch repair-deficient colorectal cancers (CRC) display widespread instability at DNA microsatellite sequences (MSI). Although MSI has been reported to commonly occur coding repeats, leading alterations in the function of a number genes encoding cancer-related proteins, nothing is known about putative impact this process on non-coding microRNAs. In miRbase V15, we identified very few human microRNA with mono- or di-nucleotide repeats (n = 27). A mutational analysis these large series CRC...
Previous work has shown that treatment of HT-29 methotrexate (MTX) cells with benzyl-N-acetyl-α-d-galactosaminide results in profound changes mucin oligosaccharide chains. To analyse depth the effect this drug, we first determined structure chains synthesized by MTX and induced permanent drug exposure. Mucins from untreated contained nine monosialylated structures (core types 1, 2, 3 4) four disialylated (types 2 4). Core 1 predominated, particular NeuAcα2–3Galβ1–3GalNAc-ol. Exposure to days...
HT‐29 sublines and Caco‐2 clones were analyzed for the expression of cytochrome P ‐450 3A. The enzyme was found to be expressed in differentiated cells selected by resistance methotrexate one seven clones, TC7. Its parallels differentiation process, with highest levels being observed at late confluency. 3A mRNA protein patterns, as well subcellular distribution, are intermediate between those human adult intestine fetal liver.