A5039991590- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Retinoids in leukemia and cellular processes
- RNA modifications and cancer
- DNA Repair Mechanisms
- T-cell and Retrovirus Studies
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Sperm and Testicular Function
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- Inflammatory mediators and NSAID effects
- Molecular Biology Techniques and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- NF-κB Signaling Pathways
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Osteoarthritis Treatment and Mechanisms
- Parvovirus B19 Infection Studies
- Phytochemical Studies and Bioactivities
- Telomeres, Telomerase, and Senescence
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- CNS Lymphoma Diagnosis and Treatment
Lebanese University
2012-2025
Centre National de la Recherche Scientifique
2002
Hôpital Necker-Enfants Malades
2002
American University of Beirut
2002
Laboratoire de Biologie du Développement
2001
Virologie et Immunologie Moléculaires
1998
Metals including iron, copper and zinc are essential for physiological processes yet can be toxic at high concentrations. However the role of these metals in progression cancer is not well defined. Here we study anti-tumor activity metal chelator, TPEN, define its mechanism action. Multiple approaches were employed, cell viability, cycle analysis, multiple measurements apoptosis, mitochondrial function. In addition measured cellular contents employed EPR to record redox cycling TPEN–metal...
Anti-Mullerian hormone (AMH) is mainly involved in the regression of Mullerian ducts male fetuses, but it may have other functions linked to gonadal development. The present study examines effect AMH on steroidogenesis by Sertoli and Leydig cells fetal immature rats during period where physiologically produced testis. basal aromatase activity primary culture was strongly stimulated (77-91%) cAMP. (35 nM) reduced cAMP-stimulated 49-69% as early day 16 until postnatal 20. This dose dependent...
Gallotannin (GT), the polyphenolic hydrolyzable tannin, exhibits anti-inflammatory and anticancer activities through mechanisms that are not fully understood. Several effects modulated by GT have been shown to be linked interference with inflammatory mediators. Considering central role of nuclear factor kappa B (NF-ĸB) in inflammation cancer, we investigated effect on NF-ĸB signaling HT-29 HCT-116 human colon cancer cells. DNA binding assays revealed significant suppression tumor necrosis...
DNA topoisomerase II (TOP2) plays a crucial role in DNA-associated processes by inducing transient double-strand breaks, making it an important target for DNA-damage stabilizing agents. Commonly used cancer therapy, these agents are designed to interfere with TOP2 cleavage complexes on chromatin. However, the epigenetic pathways influencing their effectiveness and resultant cellular responses remain elusive. Here, we combine vitro as well vivo genetic pharmacological approaches prostate...
Abstract Synthesis of eukaryotic ribosomes involves the assembly and maturation precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, proteins (RPs) a plethora factors (AFs). Formation earliest precursors 60S subunit (pre-60S r-particle) is among least understood stages ribosome biogenesis. It Npa1 complex, protein module suggested to play key role in early structuring pre-rRNA. displays genetic interactions with DExD-box Dbp7 interacts physically snR190...
The sesquiterpene lactone parthenolide is a major component of the feverfew medicinal plant, Tanacetum parthenium. Parthenolide has been extensively studied for its anti-inflammatory and anticancer properties in several tumor models. Parthenolide's antitumor activities depend on mechanisms but it mainly known as an inhibitor nuclear factor-κB (NF-κB) pathway. This pathway constitutively activated induces cell survival primary effusion lymphoma (PEL), rare aggressive AIDS-related...
Hereditary Hemorrhagic Telangiectasia syndrome (HHT) or Rendu-Osler-Weber (ROW) is an autosomal dominant vascular disorder. Two most common forms of HHT, HHT1 and HHT2, have been linked to mutations in the endoglin (ENG) activin receptor-like kinase 1 (ACVRL1or ALK1) genes respectively. This work was designed examine pathogenicity 23 nucleotide variations ACVRL1 gene detected more than 400 patients. Among them, 14 missense one intronic variant were novels, 8 previously identified with...
Eukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, of proteins. The formation maturation the earliest pre-60S particles requires structural remodeling by Npa1 complex, but is otherwise still poorly understood. Here, we introduce Rbp95 (Ycr016w), a constituent early particles, as novel factor. We show that both genetically physically linked to most complex members protein Rpl3. demonstrate an...
The plant secondary metabolite gallotannin (GT) is the simplest hydrolyzable tannin shown to have anti‐carcinogenic properties in several cell lines and inhibit tumor development different animal models. Here, we determined if GT induces senescence DNA damage investigated involvement of p53 p21 this response. Using HCT116 human colon cancer cells wildtype for +/+ /p21 null −/− or , found that independently p53. was increase production reactive oxygen species (ROS) by altering redox balance...
Primary effusion lymphoma (PEL) is a rare aggressive subset of non-Hodgkin B cell lymphoma. PEL secondary to Kaposi sarcoma herpes virus (KSHV) and predominantly develops in serous cavities. Conventional chemotherapy remains the treatment choice for yields high response rates with no significant comorbidities. Yet, often fails achieving or maintaining long-term remission. Lenalidomide (Lena), an immunomodulatory drug, displayed some efficacy PEL. On other hand, arsenic trioxide (ATO)...
The high incidence and mortality rates of colorectal cancer (CRC) reveal its hazardous effect globally. Thus, it is important to diagnose CRC at an early stage decrease burden improve survival rates. Previous studies have investigated the role short non-coding microRNAs (miRNAs or miRs) in numerous types cancer, including CRC. been performed investigate miRNAs as biomarkers diagnosis, prognosis prediction development. aim present retrospective study was identify expression levels miR-31,...
BackgroundKaposi sarcoma-associated herpesvirus (KSHV) is the etiologic agent of primary effusion lymphomas (PEL). PEL cell lines infected with KSHV, but negative for Epstein-Barr virus have a tumorigenic potential in non-obese diabetic/severe combined immunodeficient mice and result efficient engraftment formation malignant ascites notable abdominal distension, consistent clinical manifestations humans. Methodology/Principal FindingsUsing this preclinical mouse model, we demonstrate that...
Abstract Faithful DNA replication requires specific proteins that protect forks and so prevent the formation of lesions may damage genome. Identification new involved in this process is essential to understand how accumulate cancer cells they tolerate them. Here, we show human GNL3/nucleostemin, a GTP‐binding protein localized mostly nucleolus highly expressed cells, prevents nuclease‐dependent resection nascent response stress. We demonstrate inhibiting origin firing reduces resection. This...
Abstract Background Thromboxane A 2 is derived from arachidonic acid through the action of cyclooxygenases and thromboxane synthase. It mainly formed in blood platelets upon activation plays an important role aggregation. Aspirin effective reducing incidence complications following acute coronary syndrome stroke. The anti-thrombotic effect aspirin obtained irreversible inhibition cyclooxygenases. Analogues 12-hydroxyeicosatetraenoic 13-hydroxyocatdecadienoic were shown previously to modulate...