A5076397118- Cardiac electrophysiology and arrhythmias
- Chemotherapy-induced cardiotoxicity and mitigation
- RNA and protein synthesis mechanisms
- Ion channel regulation and function
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Childhood Cancer Survivors' Quality of Life
- 3D Printing in Biomedical Research
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Viral Infections and Immunology Research
- PARP inhibition in cancer therapy
- RNA Research and Splicing
- ATP Synthase and ATPases Research
- Autoimmune Neurological Disorders and Treatments
- Inflammatory mediators and NSAID effects
- Advanced NMR Techniques and Applications
- Heart Failure Treatment and Management
- Click Chemistry and Applications
- Connexins and lens biology
- Peripheral Neuropathies and Disorders
- Neuroblastoma Research and Treatments
- Bacillus and Francisella bacterial research
- ECG Monitoring and Analysis
- HIV-related health complications and treatments
Northwestern University
2019-2024
University Health Network
2024
Centre National de la Recherche Scientifique
2015-2021
Institut du Thorax
2015-2021
Inserm
2015-2021
Nantes Université
2015-2020
Lebanese University
2015
Pitié-Salpêtrière Hospital
1996
Sorbonne Université
1996
Human induced pluripotent stem cell (hiPSC) culture has become routine, yet the cost of media, frequent medium changes, and reproducibility differentiation have remained restrictive. Here, we describe formulation a hiPSC (B8) as result exhaustive optimization constituents concentrations, establishing necessity relative contributions each component to state proliferation. The reagents in B8 represent only 3% costs commercial made possible primarily by in-lab generation three E....
Genome-wide association studies and candidate gene have identified more than 180 genetic variants statistically associated with anthracycline-induced cardiotoxicity (AIC). However, the lack of functional validation has hindered clinical translation these findings. The aim this study was to functionally validate all genes AIC using human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs). Through a systemic literature search, 80 containing significantly were identified....
OBJECTIVES--To identify clinical, electrophysiological, and immunological characteristics of chronic immune demyelinating polyneuropathy to define for each group the appropriate therapeutic strategies. METHODS--The clinical electrophysiological data response treatment 93 patients with an acquired dysimmune (CDDP) studied over a period 10 years were reviewed. Two groups identified: 1, comprising 64 idiopathic CDDP, whom 13 had serum monoclonal or polyclonal gammopathy without detectable...
Background Human genetically inherited cardiac diseases have been studied mainly in heterologous systems or animal models, independent of patients' genetic backgrounds. Because sources human cardiomyocytes ( CM s) are extremely limited, the use urine samples to generate induced pluripotent stem cell–derived s would be a noninvasive method identify dysfunctions that lead pathologies within specific The objective was validate CMs differentiated from urine‐derived (UhiPS) cells as new cellular...
Background: Multiple pharmacogenomic studies have identified the synonymous genomic variant rs7853758 (G > A, L461L) and intronic rs885004 in SLC28A3 (solute carrier family 28 member 3) as statistically associated with a lower incidence of anthracycline-induced cardiotoxicity. However, true causal variant(s), cardioprotective mechanism this locus, role other solute (SLC) transporters cardiotoxicity, suitability SLC targets for drugs has not been investigated. Methods: Six well-phenotyped,...
The transplantation of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) has shown promise in preclinical models myocardial infarction, but graft myocardium exhibits incomplete host-graft electromechanical integration and a propensity for pro-arrhythmic behavior. Perhaps contributing to this situation, hPSC-CM grafts show low expression connexin 43 (Cx43), the major gap junction (GJ) protein, ventricular myocardia. We hypothesized that Cx43 function could be rescued by...
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The cardiac sodium channel Na
Abstract Anthracycline-induced cardiomyopathy is a leading cause of late morbidity in childhood cancer survivors. Aberrant DNA methylation plays role de novo cardiovascular disease. Epigenetic processes could play anthracycline-induced but remain unstudied. We sought to examine if genome-wide differential at ‘CpG’ sites peripheral blood associated with cardiomyopathy. This report used participants from matched case–control study; 52 non-Hispanic White, anthracycline-exposed survivors were...
Abstract Aims Several inherited arrhythmic diseases have been linked to single gene mutations in cardiac ion channels and interacting proteins. However, the mechanisms underlying most arrhythmias, are thought involve altered regulation of expression multiple effectors. In this study, we aimed examine role a transcription factor (TF) belonging Iroquois homeobox family, IRX5, electrical function. Methods results Using human tissues, transcriptomic correlative analyses between IRX5 genes...
Brugada syndrome is associated with loss-of-function
Background Anthracycline‐induced cardiomyopathy is a leading cause of premature death in childhood cancer survivors, presenting need to understand the underlying pathogenesis. We sought examine differential blood‐based mRNA expression profiles anthracycline‐exposed survivors with and without cardiomyopathy. Methods Results designed matched case‐control study (Children's Oncology Group‐ALTE03N1) sequencing on total RNA from peripheral blood 40 (cases) 64 (controls). DESeq2 identified...
Summary Human induced pluripotent stem cell (hiPSC) culture has become routine, yet media costs, frequent changes, and reproducibility of differentiation have remained restrictive, limiting the potential for large-scale projects. Here, we describe formulation a novel hiPSC medium (B8) as result exhaustive optimization constituents concentrations, establishing necessity relative contributions each component to state proliferation. B8 eliminates 97% costs commercial media, made possible...
Human genetically inherited cardiac diseases have mainly been studied in heterologous systems or animal models, independently of the patients’ genetic background. Because sources for human cardiomyocytes are extremely limited, use urine samples to derive would be a non-invasive method identify dysfunctions that lead pathologies within specific Cardiomyocytes differentiated from urine-derived pluripotent stem cells (UhiPS-CMs) were obtained patient with long QT syndrome and mutation hERG...
Abstract The Brugada Syndrome (BrS) is characterized by ST-segment elevation in the right precordial leads and associated with an increased risk of sudden cardiac death. disorder was initially described as a monogenic primary electrical disease. However, mutations SCN5A, encoding sodium channel (NaV1.5), which major gene are found only around 20% cases low penetrance. Furthermore many did not display familial aggregation. Based on previous GWAS conducted 312 BrS patients discovery unexpected...