A5055855538- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Cardiac Fibrosis and Remodeling
- Cardiac Arrhythmias and Treatments
- PARP inhibition in cancer therapy
- HIV-related health complications and treatments
- Cardiovascular Effects of Exercise
- Neuroscience and Neural Engineering
- Cardiovascular Function and Risk Factors
- Cytokine Signaling Pathways and Interactions
- Cellular transport and secretion
- Protein Degradation and Inhibitors
- Birth, Development, and Health
- Heart Failure Treatment and Management
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Childhood Cancer Survivors' Quality of Life
- Adipose Tissue and Metabolism
- Chemotherapy-induced cardiotoxicity and mitigation
- Atrial Fibrillation Management and Outcomes
Northwestern University
2019-2025
Sorbonne Université
2016-2021
Inserm
2016-2021
Fondation pour l’innovation en Cadiométabolisme et Nutrition
2021
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2017-2021
Université Paris Cité
2016
Human induced pluripotent stem cell (hiPSC) culture has become routine, yet the cost of media, frequent medium changes, and reproducibility differentiation have remained restrictive. Here, we describe formulation a hiPSC (B8) as result exhaustive optimization constituents concentrations, establishing necessity relative contributions each component to state proliferation. The reagents in B8 represent only 3% costs commercial made possible primarily by in-lab generation three E....
Genome-wide association studies and candidate gene have identified more than 180 genetic variants statistically associated with anthracycline-induced cardiotoxicity (AIC). However, the lack of functional validation has hindered clinical translation these findings. The aim this study was to functionally validate all genes AIC using human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs). Through a systemic literature search, 80 containing significantly were identified....
BackgroundVentricular fibrillation may be caused by premature ventricular contractions (PVCs) whose coupling intervals are <300 ms, a characteristic of the short-coupled variant torsades de pointes (scTdP).ObjectiveThe purpose this study was to analyze underlying cardiac ryanodine receptor (RyR2) variants in patients with scTdP.MethodsSeven scTdP (mean age 34 ± 12 years; 4 men and 3 women) were enrolled study. The RyR2 gene screened targeted sequencing methods; minor allele frequency...
Background: Myocarditis is clinically characterized by chest pain, arrhythmias, and heart failure, treatment for myocarditis often supportive. Mutations in DSP, a gene encoding the desmosomal protein desmoplakin, have been increasingly implicated with biomarkers pathological features indistinguishable from other forms of myocarditis. DSP-associated can progress to dilated cardiomyopathy heightened arrhythmia risk. Methods: To model cardiomyocyte aspects assess role innate immunity, we...
Preclinical models of breast cancer that better predict patient-specific drug responses are critical for expanding the clinical utility targeted therapies, including inhibitors poly(ADP-ribose) polymerase (PARP). Reprogramming primary cells into human induced pluripotent stem (hiPSCs) recently emerged as a powerful tool to model response phenotypes, but its use date has been limited hematopoietic malignancies. We designed an optimized reprogramming methodology generate cancer-derived hiPSCs...
Many cardiomyopathy-associated
Abstract Idiopathic ventricular fibrillation (IVF) causes sudden death in young adult patients without structural or ischemic heart disease. Most IVF cases are sporadic and some present with short-coupled torsade de pointes, the genetics of which poorly understood. A man who had a first syncope at age 35 presented frequent premature beats bursts polymorphic tachycardia then died suddenly. By exome sequencing, we identified three rare variants: p.I784F SPRY1 ryanodine receptor 2 (RyR2),...
Calcium regulation plays a central role in cardiac function. Several variants the calcium channel Cav1.2 have been implicated arrhythmic syndromes. We screened patients with Brugada syndrome, short QT early repolarisation and idiopathic ventricular fibrillation to determine frequency pathogenicity of variants. related genes, CACNA1C, CACNB2 CACNA2D1, were 65 probands. Missense introduced alpha subunit plasmid by mutagenesis assess their using patch clamp approaches. Six missense identified...
Catecholaminergic polymorphic ventricular tachycardia (CPVT) is triggered by exercise or acute emotion in patients with normal resting electrocardiogram. The major disease-causing gene RYR2, encoding the cardiac ryanodine receptor (RyR2). We report a novel RYR2 variant, p.Asp3291Val, outside four CPVT mutation hotspots, three families numerous sudden deaths. This missense variant was first identified four-generation family, where eight deaths occurred before age of 30 context adrenergic...
Abstract Heart failure with preserved ejection fraction (HFpEF) is commonly found in persons living HIV (PLWH) even when antiretroviral therapy (ART) suppresses viremia. However, studying this condition has been challenging because an appropriate animal model not available. In paper, we studied calcium transient human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) culture to simulate the cardiomyocyte relaxation defect noted of PLWH and HFpEF assess whether various drugs...
Heart failure with preserved ejection fraction (HFpEF) is commonly found in persons living HIV (PLWH) even when antiretroviral therapy (ART) suppresses viremia. However, studying this condition has been challenging because an appropriate animal model not available. In paper, we study the relaxation function of human induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs) culture to simulate HFpEF and assess whether various drugs have effect. We show that treatment hiPSC-CMs...
Heart failure with preserved ejection fraction (HFpEF) is commonly found in persons living HIV (PLWH) even when antiretroviral therapy suppresses viremia. However, studying this condition has been challenging because an appropriate animal model not available. In article, we studied calcium transient human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) culture to simulate the cardiomyocyte relaxation defect noted PLWH and HFpEF assess whether various drugs have effect. We...
Heart failure with preserved ejection fraction (HFpEF) is commonly found in persons living HIV (PLWH) even when antiretroviral therapy (ART) suppresses viremia. However, studying this condition has been challenging because an appropriate animal model not available. In paper, we studied calcium transient human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) culture to simulate the cardiomyocyte relaxation defect noted of PLWH and HFpEF assess whether various drugs have...
Heart failure with preserved ejection fraction (HFpEF) is commonly found in persons living HIV (PLWH) even when antiretroviral therapy suppresses viremia. However, studying this condition has been challenging because an appropriate animal model not available. In article, we studied calcium transient human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) culture to simulate the cardiomyocyte relaxation defect noted PLWH and HFpEF assess whether various drugs have effect. We...
Summary Human induced pluripotent stem cell (hiPSC) culture has become routine, yet media costs, frequent changes, and reproducibility of differentiation have remained restrictive, limiting the potential for large-scale projects. Here, we describe formulation a novel hiPSC medium (B8) as result exhaustive optimization constituents concentrations, establishing necessity relative contributions each component to state proliferation. B8 eliminates 97% costs commercial media, made possible...
Origin of idiopathic ventricular fibrillation (IVF) remains unclear. The incorporation genetic knowledge could improve the diagnosis and create a better classification this sub-group patients. To end, we collected DNA from 19 patients with VF Purkinje origin associated short-coupled premature beats or torsade de pointes. We aimed to determine frequency rare variants in 47 genes implied arrhythmias 20 additional highly expressed fibers. Exome sequencing was performed using Illumina...