A5057048465- Cardiac electrophysiology and arrhythmias
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Chemotherapy-induced cardiotoxicity and mitigation
- Ion channel regulation and function
- Childhood Cancer Survivors' Quality of Life
- CRISPR and Genetic Engineering
- Nuclear Structure and Function
- PARP inhibition in cancer therapy
- VLSI and Analog Circuit Testing
- Electrospun Nanofibers in Biomedical Applications
- ECG Monitoring and Analysis
- Tissue Engineering and Regenerative Medicine
- Neural dynamics and brain function
- Dermatologic Treatments and Research
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Microfluidic and Capillary Electrophoresis Applications
- Mitochondrial Function and Pathology
- Biotechnology and Related Fields
- Cardiomyopathy and Myosin Studies
- Systemic Sclerosis and Related Diseases
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
Northwestern University
2022-2024
Tampere University
2015-2023
University of Cambridge
2020
Tampere University of Applied Sciences
2017
FIT Biotech (Finland)
2017
University of Nottingham
2015
Exosomes are small extracellular nanovesicles that released by cells, and their potential has been explored for use in cosmetics, skin care, tissue regeneration, dermatological diseases. The therapeutic value of exosomes lies ability to modulate the microenvironment regulate gene expression, induce cell differentiation, which can have a positive impact on health. In terms used reduce wrinkles, improve texture hydration, enhance elasticity, as well inflammation damage caused UV radiation....
Genome-wide association studies and candidate gene have identified more than 180 genetic variants statistically associated with anthracycline-induced cardiotoxicity (AIC). However, the lack of functional validation has hindered clinical translation these findings. The aim this study was to functionally validate all genes AIC using human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs). Through a systemic literature search, 80 containing significantly were identified....
The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities direct fate human pluripotent stem (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, conditions required for self-renewal vs. differentiation HPSCs are different, a single system that efficiently achieves both outcomes is not available [Giobbe GG, et al. Biotechnol Bioeng 109(12):3119-3132]. We have addressed this dual need developing hydrogel-based...
Dilated cardiomyopathy (DCM) is one of the leading causes heart failure and transplantation. A portion familial DCM due to mutations in LMNA gene encoding nuclear lamina proteins lamin C without adequate treatment these patients have a poor prognosis. To get better insights into pathobiology behind this disease, we focused on modeling LMNA-related using human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CM). Primary skin fibroblasts from carrying most prevalent Finnish founder...
In the present research we tackled classification of seven genetic cardiac diseases and control subjects by using an extensive set machine learning algorithms with their variations from simple K-nearest neighbor searching method to support vector machines. The was based on calcium transient signals measured induced pluripotent stem cell-derived cardiomyocytes. All in all, 55 different alternatives were used model eight classes applying principle 10-fold crossvalidation peak data 1626...
The nutritional requirements for human induced pluripotent stem cell (hiPSC) growth have not been extensively studied. Here, building on our prior work that established the suitable non-basal medium components hiPSC growth, we develop a simplified basal consisting of just 39 components, demonstrating many ingredients DMEM/F12 are either essential or at suboptimal concentrations. This new along with supplement, which call BMEM, enhances rate hiPSCs over DMEM/F12-based media, supports...
Mutations in the HERG gene encoding potassium ion channel HERG, represent one of most frequent causes long QT syndrome type-2 (LQT2). The same genetic mutation frequently presents different clinical phenotypes family. Our study aimed to model LQT2 and functional differences between carriers variable phenotypes. We derived human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) from asymptomatic symptomatic When comparing single hiPSC-CMs, results allelic imbalance, current...
In vitro automated fabrication of living tubular architecturesThe use materials to impose tissue-like architecture at cell resolution will be important if engineered functional replacements for damaged cardiovascular, pulmonary, renal or digestive tissues are authentically engineered.Here, we demonstrate a coordinated system the and subsequent culture composed multiple layers, cell-types with physiological dimensions defined architectures resolution.We developed an tube fabricator (ATF) that...
Abstract Anthracycline-induced cardiomyopathy is a leading cause of late morbidity in childhood cancer survivors. Aberrant DNA methylation plays role de novo cardiovascular disease. Epigenetic processes could play anthracycline-induced but remain unstudied. We sought to examine if genome-wide differential at ‘CpG’ sites peripheral blood associated with cardiomyopathy. This report used participants from matched case–control study; 52 non-Hispanic White, anthracycline-exposed survivors were...
Abstract Enhancer-promoter dynamics are crucial for the spatiotemporal control of gene expression, but it remains unclear whether these controlled by chromatin regulators, such as nucleosome remodelling and deacetylase (NuRD) complex. The NuRD complex binds to all active enhancers modulate transcription here we use Hi-C experiments show that blurs TAD boundaries increases proximity intermediate-range (~1 Mb) genomic sequences enhancer-promoter interactions. To understand alters 3D genome...
Microelectrode arrays (MEAs) are widely used to assess the electrophysiology of human pluripotent stem cell-derived cardiomyocytes (hPS-CMs). Traditionally, MEAs have been record data at cell population level, but it would be beneficial able analyze also single-cell level using MEAs. To realize this, we present a special MEA platform for recording field potential from single beating cardiomyocytes. The size and location transparent indium tin oxide (ITO) electrodes optimized make noninvasive...
ABSTRACT Long QT syndrome (LQTS) is characterized by a prolonged QT-interval on electrocardiogram and increased risk of sudden death. One the most common potentially life-threatening electrolyte disturbances hypokalemia, low concentrations K+. Using multielectrode array platform current clamp technique, we investigated effect extracellular K+ concentration ([K+]Ex) electrophysiological properties hiPSC-derived cardiomyocytes (CMs) generated from healthy control subject (WT) two symptomatic...
Brugada syndrome is an inherited cardiac arrhythmia disorder that mainly associated with mutations of the voltage-gated sodium channel alpha subunit 5 (SCN5A) gene. The clinical symptoms include ventricular fibrillation and increased risk sudden death. Human-induced pluripotent stem cell (hiPSC) lines were derived from symptomatic asymptomatic individuals carrying R1913C mutation in SCN5A present work aimed to observe phenotype-specific differences hiPSC-derived cardiomyocytes (CMs) obtained...
We study complex scaling properties of RR and QT intervals electrocardiograms (ECGs) with their equivalences at the cellular level, that is, inter-beat (IBI) field potential durations (FPD) spontaneously beating human-induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) aggregates. Our detrended fluctuation analysis Poincaré plots reveal remarkable similarities between ECG hiPSC-CM data. In particular, no statistically significant difference was found in short- long-term exponents...
Biologics have helped treatment of diseases including cancers, rheumatoid arthritis, etc. in entirely new ways. Because they are expensive, there is a demand for generic versions (biosimilars). Biologicals and biosimilars consist large complex molecular entities difficult to characterize. An innovator biosimilar will never be identical because their manufacturing processes different; hence both need evaluated potential adverse effects clinical impact, thus require separate regulatory...
Brugada syndrome is associated with loss-of-function
Cardiomyocytes differentiated from human induced pluripotent stem cells (iPSC-CMs) can be used to study genetic cardiac diseases. In patients these diseases are manifested e.g. with impaired contractility and fatal arrhythmias, both of due abnormal calcium transients in cardiomyocytes. Here we classify different using Ca2+ transient data machine learning algorithms. By studying cycling disease-specific iPSC-CMs by measured it is possible each other also healthy controls applying computation...
Background Anthracycline‐induced cardiomyopathy is a leading cause of premature death in childhood cancer survivors, presenting need to understand the underlying pathogenesis. We sought examine differential blood‐based mRNA expression profiles anthracycline‐exposed survivors with and without cardiomyopathy. Methods Results designed matched case‐control study (Children's Oncology Group‐ALTE03N1) sequencing on total RNA from peripheral blood 40 (cases) 64 (controls). DESeq2 identified...
Abstract In this article, we consider data complexity in the context of calcium transient signal collected from induced pluripotent stem cell‐derived cardiomyocytes. We present a novel way to measure based on nearest neighbour searching method. Data here is seen as overlapping and mixed classes addition relatively great number cases. Complexity affects classification results, which were run with searching, feedforward artificial neural networks random forests for seven genetic cardiological...
Event Abstract Back to Mini-incubator for prolonged hypoxia studies on MEA: Effect of IPSC-derived cardiomyocytes Joose Kreutzer1*, Disheet Shah2, Kaisa Tornberg1, Antti-Juhana Mäki1, Mari Pekkanen-Mattila2, Katriina Aalto-Setälä2 and Pasi Kallio1 1 Tampere University Technology, BioMediTech Institute Faculty Biomedical Sciences Engineering, Finland 2 Tampere, BioMediTech, Motivation Cells are normally cultured inside an incubator where 5% from atmospheric air is replaced by CO2. That leads...
We characterize the complexity of interbeat intervals (IBIs) and field potential durations (FPDs) humaninduced plurioptent stem cell-derived cardiomyocytes (hiPSC-CMs).The is assessed using multiscale entropy (MSE) method up to scale ten beats.The MSE profiles healthy diseased (long-QT syndrome) hiPSC-CMs show important differences, which demonstrate usefulness in characterization cells analysis hereditary cardiac diseases.We that intrinsic possibly altered by differentiation methods, as...
Traditional protocols for the differentiation of hiPSC-derived cardiomyocytes (hiPSC-CMs) rely on complex media and use animal or recombinant proteins either directly in supplements, such as B27, to obtain tissue monolayers. This fact decreases their reproducibility, while potentially increasing costs limiting higher-throughput production applications both regenerative medicine, disease modeling, pharmacological screenings. Albumin/serum-free have been developed but produce cells with...