To promote the transition of cell cultures from 2D to 3D, hydrogels are needed biomimic extracellular matrix (ECM). One potential material for this purpose is gellan gum (GG), a biocompatible and mechanically tunable hydrogel. However, GG alone does not provide attachment sites cells thrive in 3D. option biofunctionalization introduction gelatin, derivative abundant ECM protein collagen. Unfortunately, gelatin lacks cross-linking moieties, making production self-standing difficult under...

Mutations in the HERG gene encoding potassium ion channel HERG, represent one of most frequent causes long QT syndrome type-2 (LQT2). The same genetic mutation frequently presents different clinical phenotypes family. Our study aimed to model LQT2 and functional differences between carriers variable phenotypes. We derived human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) from asymptomatic symptomatic When comparing single hiPSC-CMs, results allelic imbalance, current...

Sialylated glycans serve as key elements of receptors for many viruses, bacteria, and bacterial toxins. The microbial recognition their binding specificity can be affected by the linkage terminal sugar residue, types underlying chains, nature entire glycoconjugate. Owing to pathobiological significance sialylated glycans, we have engineered Chinese hamster ovary (CHO) cells secrete mucin-type immunoglobulin-fused proteins carrying α2,3- or α2,6-linked sialic acid on defined O-glycan core...

The binding of Shiga-like toxin 1 (Stx1) and 2 (Stx2) to a mucin-like fusion protein, P-selectin glycoprotein ligand-1/mouse IgG2b (PSGL-1/mIgG2b), carrying multiple copies the blood group P1 determinant on O-glycans was investigated with western blot biosensor Biacore. Chinese hamster ovary K-1 (CHO-K1) cells were stably transfected linearized plasmids encoding PSGL-1/mIgG2b pigeon α1,4-galactosyltransferase (α4Gal-T) core β1,6-N-acetylglucosaminyltransferase (C2GnT-I). Western analyses...

Abstract Animal bioprosthetic heart valves (BHV) are used to replace defective in patients with valvular disease. Especially young BHV recipients may experience a structural valve deterioration caused by an immune reaction which α-Gal and Neu5Gc potential target antigens. The expression of these other carbohydrate antigens animal tissues for production was explored. Protein lysates porcine aortic pulmonary valves, porcine, bovine equine pericardia were analyzed Western blotting using...

Ischemic heart disease is the most common cardiovascular and a major burden for healthcare worldwide. However, its pathophysiology still not fully understood, human-based models mechanisms treatments are needed. Here, we used human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to model acute ischemia-reperfusion in our novel cell culture assembly. The assembly enables exchange of oxygen partial pressure cells within minutes, mimicking ischemic event. In this study, hypoxia...

Brugada syndrome is an inherited cardiac arrhythmia disorder that mainly associated with mutations of the voltage-gated sodium channel alpha subunit 5 (SCN5A) gene. The clinical symptoms include ventricular fibrillation and increased risk sudden death. Human-induced pluripotent stem cell (hiPSC) lines were derived from symptomatic asymptomatic individuals carrying R1913C mutation in SCN5A present work aimed to observe phenotype-specific differences hiPSC-derived cardiomyocytes (CMs) obtained...

Hypertrophic cardiomyopathy (HCM) is one of the most common genetic cardiac diseases; it primarily caused by mutations in sarcomeric genes. However, HCM also associated with non-sarcomeric proteins and a Finnish founder mutation for protein junctophilin-2 (JPH2) has been identified. This study aimed at assessing issue modelling rare cardiomyocytes (CMs) differentiated from iPSCs; therefore, presenting same abnormalities observed patients. To explore abnormal functions JPH2-HCM, skin...

ABSTRACT The capability of a recombinant mucin-like fusion protein, P-selectin glycoprotein ligand-1/mouse IgG2b (PSGL-1/mIgG2b), carrying Galα1,3Galβ1,4GlcNAc determinants to bind and inhibit Clostridium difficile toxin A (TcdA) was investigated. produced by glyco-engineered stable CHO-K1 cell line designated C-PGC2, purified affinity gel filtration chromatography from large-scale cultures. Liquid chromatography-mass spectrometry used characterize O -glycans released reductive...

The hexameric MCM complex is the catalytic core of replicative helicase in eukaryotic and archaeal cells. Here we describe first vivo analysis protein structure function relationships using genetically tractable haloarchaeon Haloferax volcanii as a model system. Hfx. encodes single that part previously identified group haloarchaeal proteins. Three structural features N-terminal domain were targeted for mutagenesis: β7-β8 β9-β10 β-hairpin loops putative zinc binding domain. Five strains...

Abstract Funding Acknowledgements Type of funding sources: Private grant(s) and/or Sponsorship. Main source(s): 1- Central Department Missions, Egyptian Ministry Higher education and Scientific Research. 2- small fund from Tampere University. The contractility the heart is based on cardiomyocytes (CM). These are connected through a complex network proteins forming intercalated disc (ID) which crucial to ensure mechanical electrochemical coupling. Dilated cardiomyopathy (DCM) disease...

Abstract Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) hold great potential in the cardiovascular field for human disease modeling, drug development, and regenerative medicine. However, multiple hurdles still exist effective utilization of hiPSC-CMs as a human-based experimental platform that can be an alternative to current animal models. To further expand their research tool bridge translational gap, we have generated cardiac-specific hiPSC reporter line...