A5024611423- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Polysaccharides Composition and Applications
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Optical Imaging and Spectroscopy Techniques
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Collagen: Extraction and Characterization
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- biodegradable polymer synthesis and properties
- Silk-based biomaterials and applications
- Laser Applications in Dentistry and Medicine
- CRISPR and Genetic Engineering
- Advanced Materials and Mechanics
- Pickering emulsions and particle stabilization
- Rheumatoid Arthritis Research and Therapies
- Nanoparticle-Based Drug Delivery
- Clay minerals and soil interactions
- Design Education and Practice
- Advanced Drug Delivery Systems
- Spondyloarthritis Studies and Treatments
Tampere University
2016-2025
Karolinska Institutet
2021-2023
University of Oulu
2019-2022
National Research Centre for the Working Environment
2019
Tampere University
2017
To promote the transition of cell cultures from 2D to 3D, hydrogels are needed biomimic extracellular matrix (ECM). One potential material for this purpose is gellan gum (GG), a biocompatible and mechanically tunable hydrogel. However, GG alone does not provide attachment sites cells thrive in 3D. option biofunctionalization introduction gelatin, derivative abundant ECM protein collagen. Unfortunately, gelatin lacks cross-linking moieties, making production self-standing difficult under...
Neural tissue engineering and three-dimensional in vitro modeling require the development of biomaterials that take into account specified requirements human neural cells tissue. In this study, an alternative method producing biomimetic hydrogels based on gellan gum (GG) was developed by replacing traditional crosslinking methods with bioamines spermidine spermine. These were proven to function as crosslinkers for GG hydrogel at +37 °C, allowing encapsulation neurons. We studied mechanical...
Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most them at physiological pH has hindered their applications. To prepare a water-soluble derivative chitosan (WSC) biopolymer, it was grafted with small anionic amino acid, l-glutamic using single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction. This resulted in zwitterion-tethered structure onto polymer backbone. The degree substitution range 13-16 ± 1.25%, which...
A functional bioink with potential in bone tissue engineering must be subjected to critical investigation throughout its intended lifespan. The aim of this study was develop alginate-gelatin-based (Alg-Gel) multicomponent bioinks systematically and assess the short- long-term exposure responses human marrow stromal cells (hBMSCs) printed within these without crosslinking.The first generation bioinkswas established by incorporating a range cellulose nanofibrils (CNFs), evaluate their effect...
Abstract New strategies for spatially controlled growth of human neurons may provide viable solutions to treat and recover peripheral or spinal cord injuries. While topography cues are known promote attachment direct proliferation many cell types, guided outgrowth neurites has been found difficult achieve so far. Here, three-dimensional (3D) micropatterned carbon nanotube (CNT) templates used effectively neurite stem growth. By exploiting the mechanical flexibility, electrically conductivity...
Recently, the hydrogel-forming polysaccharide gellan gum (GG) has gained popularity as a versatile biomaterial for tissue engineering purposes. Here, we examine modification strategies suitable GG to overcome processing-related limitations. We emphasize thorough assessment of viscoelastic and mechanical properties both precursor solutions final hydrogels. The investigated include purification, oxidation, reductive chain scission, blending. correlate polymer flow hydrogel forming capabilities...
An Optical Projection Tomography (OPT) system was developed and optimized to image 3D tissue engineered products based in hydrogels. We develop pre-reconstruction algorithms get the best result from reconstruction procedure, which include correction of illumination determination sample center rotation (CoR). Existing methods for CoR on detection maximum variance reconstructed slices failed, so we a new search method sharpest local maximum. show capabilities give quantitative information...
The microstructure and permeability are crucial factors for the development of hydrogels tissue engineering, since they influence cell nutrition, penetration, proliferation. currently available imaging methods able to characterize have many limitations. They often require sample drying other destructive processing, which can change hydrogel structure, or limited penetration depth. In this work, we show first time an alternative nondestructive method, based on optical projection tomography...
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have the potential to serve as a model for human cardiomyocytes. However, hiPSC-CMs are still considered immature. CMs differentiated from hiPSCs more resemble fetal than adult Putative factors enhancing maturation include in vitro culture duration, surface topography, and mechanical, chemical, electrical stimulation. Stem traditionally cultured on glass surfaces coated with extracellular matrix derivatives such gelatin....
Abstract Assessing cell morphology and function, as well biomaterial performance in cultures, is one of the key challenges biology tissue engineering (TE) research. In TE, there an urgent need for methods to image actual three-dimensional (3D) cultures access living cells. This difficult using established optical microscopy techniques such wide-field or confocal microscopy. To address problem, we have developed a new protocol Optical Projection Tomography (OPT) extract quantitative...
Abstract There is a clear need for novel in vitro models, especially neuronal applications. Development of models multiparameter task consisting cell‐, biomaterial‐, and environment‐related parameters. Here, three different human origin cell sources are studied cultured various hydrogel 3D scaffolds. For the efficient evaluation complex results, an indexing method data developed used principal component analysis (PCA). It found that no single superior to other hydrogels, collagen I (Col1)...
Injectable scaffolds are a promising strategy to restore and regenerate damaged diseased tissues. They require minimally invasive procedure allow the formation of an in-situ structure any shape. However, 3D with aligned morphologies using method which could be easily transferred clinical settings remains challenge. Herein, rational design injectable hydrogel-based scaffold via remote-induced alignment is reported. Carboxylated multi-walled carbon nanotubes (cMWCNT) into hydrogel low magnetic...
Polymerized high internal phase emulsions (polyHIPEs) were combined with gellan gum (GG) in an innovative approach. Four GG concentrations polyHIPEs (P-GG 0%, P-GG 0.1%, 0.5%, and 1%) explored for their impact on polyHIPE materials. The resulting macroporous polyHIPE-GG (P-GG) scaffolds exhibited up to 95% porosity remarkable interconnectivity. Elevating concentration correlated larger pore sizes, increased hydrophilicity, degradability. Scanning electron microscopy (SEM) X-ray microcomputed...
This article proposes the coupling of recombinant protein avidin to polysaccharide gellan gum create a modular hydrogel substrate for 3D cell culture and tissue engineering. Avidin is capable binding biotin, thus biotinylated compounds can be tethered polymer network improve response. The successfully conjugated remains functional as shown with fluorescence titration electrophoresis (SDS-PAGE). Self-standing hydrogels were formed using bioamines calcium chloride, yielding long-term stability...
Graphical Abstract Schematic of the crosslinking process gellan gum by Ca 2+ and Mg released from bioactive glass.
Abstract Self‐healing composite hydrogels are prepared from sustainable biopolymers by a green chemistry approach and analyzed physicochemical mechanical characterization techniques for future injectable anticancer biomaterials. Water‐soluble chitosan (WSC) was grafting polyethylene glycol (PEG), glutamic acid gallic onto the chain carbodiimide chemistry. This WSC showed fast gelation ( t ≈ < 60 seconds) with benzaldehyde‐terminated 4‐arm‐PEG as crosslinker through an amine/aldehyde...
Abstract Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) hold great potential in the cardiovascular field for human disease modeling, drug development, and regenerative medicine. However, multiple hurdles still exist effective utilization of hiPSC-CMs as a human-based experimental platform that can be an alternative to current animal models. To further expand their research tool bridge translational gap, we have generated cardiac-specific hiPSC reporter line...