A5008780721- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Optical Imaging and Spectroscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Medical Imaging Techniques and Applications
- Physiological and biochemical adaptations
- Metabolomics and Mass Spectrometry Studies
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- vaccines and immunoinformatics approaches
- Nuclear Structure and Function
- Advanced Fluorescence Microscopy Techniques
- Bacteriophages and microbial interactions
- Optics and Image Analysis
- Cell Image Analysis Techniques
- Microscopic Colitis
- Intestinal and Peritoneal Adhesions
- Silk-based biomaterials and applications
- RNA Interference and Gene Delivery
- Optical Systems and Laser Technology
- Electrical and Bioimpedance Tomography
- Tissue Engineering and Regenerative Medicine
- Optical Coherence Tomography Applications
- Morphological variations and asymmetry
- Celiac Disease Research and Management
Tampere University
2017-2023
La Trobe University
2023
Tampere University
2017
Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response conformational transitions of proteins, are great interest cancer research treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix. The proposed SF presented viscoelastic nature spontaneous changes from...
The enzyme transglutaminase 2 (TG2) plays a key role in celiac disease (CeD) pathogenesis. Active TG2 is located mainly extracellularly the lamina propria but also villous enterocytes of duodenum. inhibitor ZED1227 promising drug candidate for treating CeD and designed to block TG2-catalyzed deamidation crosslinking gliadin peptides. Our aim was study accumulation after oral administration drug. We studied duodenal biopsies derived from phase 2a clinical trial using an antibody that detects...
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell line was estimated be up 15°C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis,...
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell line was estimated be up 15°C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis,...
Abstract Assessing cell morphology and function, as well biomaterial performance in cultures, is one of the key challenges biology tissue engineering (TE) research. In TE, there an urgent need for methods to image actual three-dimensional (3D) cultures access living cells. This difficult using established optical microscopy techniques such wide-field or confocal microscopy. To address problem, we have developed a new protocol Optical Projection Tomography (OPT) extract quantitative...
Investigation of nuclear lamina architecture relies on superresolved microscopy. However, epitope accessibility, labeling density, and detection precision individual molecules pose challenges within the molecularly crowded nucleus. We developed iterative indirect immunofluorescence (IT-IF) staining approach combined with expansion microscopy (ExM) structured illumination to improve superresolution subnuclear nanostructures like lamins. prove that ExM is applicable in analyzing highly...
This study focuses on improving the reconstruction process of brightfield optical projection tomography (OPT). OPT is often described as equivalent X-ray computed tomography, but based visible light. The detection optics used to collect light in focus a certain distance and induce blurring those features out focus. However, conventionally inverse Radon transform assumes an absolute throughout propagation axis. In this study, we model focusing properties by coupling Gaussian beam (GBM) with...
Solving the fluorophore distribution in a tomographic setting has been difficult because of lack physically meaningful and computationally applicable propagation models. This study concentrates on direct modelling fluorescence signals optical projection tomography (OPT), corresponding inverse problem. The reconstruction problem is solved using emission projections to series rotational imaging positions sample. Similarly bright field OPT bearing resemblance with transmission x-ray computed...
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow, and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell-line was estimated be up 15 °C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis, adenine...
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow, and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell-line was estimated be up 15 °C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis, adenine...
The antibiotic-tolerant biofilms present in tuberculous granulomas add an additional layer of complexity when treating mycobacterial infections, including tuberculosis (TB). For a more efficient treatment TB, the biofilm forms mycobacteria warrant specific attention. Here, we used Mycobacterium marinum (Mmr) as biofilm-forming model to identify abundant proteins covering surface. We biotinylation/streptavidin-based proteomics on exposed at Mmr matrices vitro 448 and ex vivo detect 91 from...
Abstract Investigation of nuclear lamina architecture relies on super-resolved microscopy. However, epitope accessibility, labeling density, and detection precision individual molecules pose challenges within the molecularly crowded nucleus. We developed iterative indirect immunofluorescence (IT–IF) staining approach combined with expansion microscopy (ExM) structured illumination to improve super-resolution subnuclear nanostructures like lamins. prove that ExM is applicable in analyzing...
ABSTRACT Based on studies with a fluorescent reporter dye, Mito Thermo Yellow, and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell-line was estimated be up 15 °C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis,...