A5036093682- Thermoregulation and physiological responses
- Optical Imaging and Spectroscopy Techniques
- Infrared Thermography in Medicine
- Photoacoustic and Ultrasonic Imaging
- Cardiovascular Health and Disease Prevention
- Non-Invasive Vital Sign Monitoring
- Flow Measurement and Analysis
- 3D Printing in Biomedical Research
- Hemodynamic Monitoring and Therapy
- Heart Rate Variability and Autonomic Control
- Gold and Silver Nanoparticles Synthesis and Applications
- Optical Coherence Tomography Applications
- Advanced Fluorescence Microscopy Techniques
- Plasmonic and Surface Plasmon Research
- Electrical and Bioimpedance Tomography
- Climate Change and Health Impacts
- Microfluidic and Bio-sensing Technologies
- Advanced Image Fusion Techniques
- Quantum Dots Synthesis And Properties
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Medical Imaging Techniques and Applications
- Photochromic and Fluorescence Chemistry
- Nonlinear Optical Materials Studies
- Ocular and Laser Science Research
Champalimaud Foundation
2019-2023
International Iberian Nanotechnology Laboratory
2016-2019
Laboratoire d'Ingénierie des Systèmes
2010-2018
Laboratoire d'Informatique et d'Automatique pour les Systèmes
2010-2018
Laboratoire Techniques, Territoires et Sociétés
2010-2018
Laboratoire Interuniversitaire des Systèmes Atmosphériques
2010-2018
QuantaLab
2018
Clinical Academic Center of Braga
2017-2018
Tampere University
2017
Tampere University
2014-2016
An Optical Projection Tomography (OPT) system was developed and optimized to image 3D tissue engineered products based in hydrogels. We develop pre-reconstruction algorithms get the best result from reconstruction procedure, which include correction of illumination determination sample center rotation (CoR). Existing methods for CoR on detection maximum variance reconstructed slices failed, so we a new search method sharpest local maximum. show capabilities give quantitative information...
The microstructure and permeability are crucial factors for the development of hydrogels tissue engineering, since they influence cell nutrition, penetration, proliferation. currently available imaging methods able to characterize have many limitations. They often require sample drying other destructive processing, which can change hydrogel structure, or limited penetration depth. In this work, we show first time an alternative nondestructive method, based on optical projection tomography...
Abstract Assessing cell morphology and function, as well biomaterial performance in cultures, is one of the key challenges biology tissue engineering (TE) research. In TE, there an urgent need for methods to image actual three-dimensional (3D) cultures access living cells. This difficult using established optical microscopy techniques such wide-field or confocal microscopy. To address problem, we have developed a new protocol Optical Projection Tomography (OPT) extract quantitative...
Laser speckle contrast imaging (LSCI) is a non-invasive microvascular blood flow assessment technique with good temporal and spatial resolution. Most LSCI systems, including commercial devices, can perform only qualitative evaluation, which major limitation of this technique. There are several factors that prevent the utilization as quantitative Among these factors, we highlight effect static scatterers. The goal work was to study influence differences in dynamic scatterer concentration on...
We demonstrate for the first time that an ultra-broadband 7 femtosecond (fs) few-cycle laser can be used multicolor nonlinear imaging in a single channel detection geometry, when employing time-resolved fluorescence scheme. On multi-chromophore-labelled cell sample we show efficiently excite multiple chromophores over >400 nm two-photon absorption range. By combining excitation with time-correlated single-photon counting (TCSPC) to record lifetime microscopy (FLIM) images, localization of...
Local pulse-wave velocity (PWV) is an accurate indicator of the degree arteriosclerosis (stiffness) in artery, providing a direct characterization properties its wall. Devices currently available for local PWV measurement are mainly based on ultrasound systems and have not yet been generalized to clinical practice since they require high technical expertise most them limited precision, due lack reliable signal processing methods. The present work describes new type probe, double-headed...
The enhancement and the control of quantum dot (QD) light emission is a challenge for many applications.
Electrical impedance tomography (EIT) is an imaging method that could become a valuable tool in multimodal applications. One challenge simultaneous typically the EIT electrodes cover large portion of object surface. This paper investigates feasibility rotational (rEIT) applications where only limited angle surface object. In studied rEIT, rotated full 360° during set measurements to increase information content data. We call this approach revolution rEIT (LAFR-rEIT). test LAFR-rEIT setups...
INL received support for this project from the CCDR-N via 'Nanotechnology based functional solutions' (Grant No. NORTE-01-0145-FEDER-000019) and Portuguese Foundation Science Technology (FCT) 'ON4SupremeSens' PTDC/NAN-OPT/29417/2017. Edite Figueiras a Marie Curie fellowship EU-EC COFUND program 'NanoTRAINforGrowth' 600375). U Minho research was partially supported by FCT in framework of Strategic Funding UID/FIS/04650/2013.
This study focuses on improving the reconstruction process of brightfield optical projection tomography (OPT). OPT is often described as equivalent X-ray computed tomography, but based visible light. The detection optics used to collect light in focus a certain distance and induce blurring those features out focus. However, conventionally inverse Radon transform assumes an absolute throughout propagation axis. In this study, we model focusing properties by coupling Gaussian beam (GBM) with...
Solving the fluorophore distribution in a tomographic setting has been difficult because of lack physically meaningful and computationally applicable propagation models. This study concentrates on direct modelling fluorescence signals optical projection tomography (OPT), corresponding inverse problem. The reconstruction problem is solved using emission projections to series rotational imaging positions sample. Similarly bright field OPT bearing resemblance with transmission x-ray computed...
Laser Doppler flowmetry (LDF) is now commonly used in clinical research to monitor microvascular blood flow. However, the dependence of LDF signal on architecture still unknown. That why we propose a new laser flowmeter for depth dependent monitoring skin perfusion. This combines first time, device, several wavelengths and different spaced detection optical fibres. The calibration apparatus herein presented together with vivo validation. Two validation tests are performed. In test, signals...
A new multi-wavelength laser Doppler flowmeter prototype with different spaced detection optical fibres, built in order to give depth discrimination capabilities flowmetry technique (LDF), was developed. The Fourier transform and the power spectral density of LDF signals measured by were calculated analyze frequency oscillations present human microcirculatory blood flow explore potential for flood skin discrimination. dependence also using coherence function. Our results show that perfusion...
Laser Doppler flowmetry (LDF) is a technique for real-time assessment of microcirculation blood flow. LDF can be used non-invasive perfusion monitoring (for example skin monitoring), or invasive evaluations brain evaluations). Using multi-wavelength laser flowmeter prototype with different spaced detection fibers, we propose to add depth discrimination capabilities monitoring. We also build self-mixing based estimation. These two prototypes will solve drawbacks prevailing in the flowmeters...
The present paper shows a method for pulse waveform extraction using laser speckle contrast analysis. An experimental apparatus was assembled, coherent light source and digital video camera to record time varying patterns emitted from the radial artery. data were analysed by computing pattern on sequence of frames. wave signal then compared with photoplethysmographic both frequency domain. A total thirty data-sets acquired 10 individuals. Subjects heart rate identified root mean square error...