A5047827363- Nitric Oxide and Endothelin Effects
- Neuroscience and Neuropharmacology Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Mitochondrial Function and Pathology
- Biochemical effects in animals
- Electrochemical sensors and biosensors
- Neurological Disease Mechanisms and Treatments
- Neuroscience of respiration and sleep
- Photoreceptor and optogenetics research
- Optical Imaging and Spectroscopy Techniques
- Neuroscience and Neural Engineering
- Anesthesia and Neurotoxicity Research
- Electrochemical Analysis and Applications
- Alzheimer's disease research and treatments
- Electron Spin Resonance Studies
- Conducting polymers and applications
- Eicosanoids and Hypertension Pharmacology
- Analytical Chemistry and Sensors
- Neural dynamics and brain function
- Advanced MRI Techniques and Applications
- Antioxidant Activity and Oxidative Stress
- Phytochemicals and Antioxidant Activities
- Hemoglobin structure and function
- Neonatal and fetal brain pathology
- EEG and Brain-Computer Interfaces
University of Coimbra
2015-2024
Centro de Neurociências e Biologia Celular
2018-2022
University of Minho
2019
Universidade do Porto
2005-2008
Cyclosporine A (CsA) has represented a fundamental therapeutic weapon in immunosupression for the past three decades. However, its clinical use is not devoid of side effects, among which hypertension and vascular injury represent major drawback. Endothelial cells are able to generate reactive oxygen nitrogen species upon exposure CsA, including formation peroxynitrite. This may result endothelial cell toxicity increased tyrosine nitration. We have now studied subcellular origin superoxide...
Hemispheric functional asymmetries are a key aspect of human brain organization. Revealing how causal manipulations hemispheric (im)balances dynamically change pre and postsynaptic GABA regulation, in terms mean variance changes, is essential to understand plasticity health disease. This has direct implications for treatment approaches stroke relying on interhemispheric inhibition using transcranial magnetic stimulation (TMS). We investigated neurobehavioral neurochemical effects cortical...
During the last decades nitric oxide ( • NO) has emerged as a critical physiological signaling molecule in mammalian tissues, notably brain. NO may modify activity of regulatory proteins via direct reaction with heme moiety, or indirectly, S-nitrosylation thiol groups nitration tyrosine residues. However, conceptual understanding how bioactivity is carried out biological systems hampered by lack knowledge on its dynamics vivo . Key questions still lacking concrete and definitive answers...
Neurovascular and neurometabolic coupling are critical complex processes underlying brain function. Perturbations in the regulation of these are, likely, early dysfunctional alterations pathological aging age-related neurodegeneration. Evidences support role nitric oxide (•NO) as a key messenger both neurovascular coupling, by signaling from neurons to blood vessels, modulating O2 utilization mitochondria. In present study, we investigated functionality connection •NO association cognitive...
To identify dietary phenolic compounds capable of improving vitamin E status, male Sprague-Dawley rats were fed for 4 weeks either a basal diet (control) with 2 g/kg cholesterol and an adequate content or the fortified quercetin (Q), (−)-epicatechin (EC), (+)-catechin (C) at concentrations g/kg. All three catechol derivatives substantially increased α-tocopherol (α-T) in blood plasma liver. study potential mechanisms underlying observed increase α-T, capacities flavonoids to i) protect α-T...
Ceramic-based multisite Pt microelectrode arrays (MEAs) were characterized for their basic electrochemical characteristics and used in vivo measurements of oxygen with high resolution the brain extracellular space. The array sites showed a very smooth surface mainly composed thin-film polycrystalline Pt, some apparent nanoscale roughness that was not translated into an increased active area. cyclic voltammetric behavior characteristic bulk both acidic neutral media. In addition, complex...
Spreading depolarization (SD) is a slow propagating wave of strong neural cells, implicated in several neuropathological conditions. The breakdown brain homeostasis promotes significant hemodynamic and metabolic alterations, which impacts on neuronal function. In this work we aimed to develop an innovative multimodal approach, encompassing metabolic, electric measurements, tailored but not limited study SD. This was based novel dual-biosensor microelectrode arrays designed simultaneously...
Background: Ample evidence from clinical and pre-clinical studies suggests mid-life hypercholesterolemia as a risk factor for developing Alzheimer’s disease (AD) at later age. Hypercholesterolemia induced by dietary habits can lead to vascular perturbations that increase the of sporadic AD. Objective: To investigate effects high fat/cholesterol diet (HFCD) AD using rodent model its correspondent control (healthy animals). Methods: We compared effect HFCD in normal mice (non-transgenic mice,...
Diet is currently recognized as a major modifiable agent of human health. In particular, dietary nitrate has been increasingly explored strategy to modulate different physiological mechanisms with demonstrated benefits in multiple organs, including gastrointestinal, cardiovascular, metabolic, and endocrine systems. An intriguing exception this scenario the brain, for which evidence remains controversial. Upon consumption, can undergo sequential reduction reactions vivo produce nitric oxide...
Dietary phenolic compounds may act as antioxidants in vitro, but because of structural modifications during absorption, its role based on concentrations high enough to afford an antioxidant protection needs be re-evaluated. We have explored the hypothesis that red wine procyanidins interact with low density lipoproteins (LDL) and that, at this location, efficiently protect LDL from oxidation maintain α-tocopherol a steady state concentration by recycling it back α-tocopheroxyl radical. To...
Abstract Nitric oxide ( • NO) is a labile endogenous free radical produced upon glutamatergic neuronal activity in hippocampus by nitric synthase (nNOS), where it acts as modulator of both synaptic plasticity and cell death associated with neurodegeneration. The low CNS levels fast time dynamics this molecule require the use rapid analytical methods that can more accurately describe its signaling vivo. This critical for understanding how kinetics NO‐dependent pathways translated into...
The mechanisms underlying nitric oxide (•NO) synthesis and inactivation in the brain are essential determinants of •NO neuroactivity. Although production is well characterized, pathways vivo remain largely unknown. Here, we characterize kinetics major mechanism rat cortex hippocampus by measuring locally applied with carbon-fiber microelectrodes (CFMs) ceramic-based microelectrode arrays (MEAs). An apparent first-order clearance was observed both regions, decay rate constants (k) signals...