A5049043406- Optical Imaging and Spectroscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- 3D Printing in Biomedical Research
- Electrical and Bioimpedance Tomography
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Reproductive Biology and Fertility
- Digital Holography and Microscopy
- Advanced Image Fusion Techniques
- Medical Imaging Techniques and Applications
- Advanced Image Processing Techniques
- Electrospun Nanofibers in Biomedical Applications
- Cell Image Analysis Techniques
- Bone Tissue Engineering Materials
- Computer Graphics and Visualization Techniques
- Collagen: Extraction and Characterization
- Medicinal Plant Pharmacodynamics Research
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- Silk-based biomaterials and applications
- Advanced Fluorescence Microscopy Techniques
- Cancer-related molecular mechanisms research
Tampere University
2017-2023
International Iberian Nanotechnology Laboratory
2023
To promote the transition of cell cultures from 2D to 3D, hydrogels are needed biomimic extracellular matrix (ECM). One potential material for this purpose is gellan gum (GG), a biocompatible and mechanically tunable hydrogel. However, GG alone does not provide attachment sites cells thrive in 3D. option biofunctionalization introduction gelatin, derivative abundant ECM protein collagen. Unfortunately, gelatin lacks cross-linking moieties, making production self-standing difficult under...
Three-dimensional (3D) cancer models are revolutionising research, allowing for the recapitulation of an in vivo-like response through use vitro system, which is more complex and physiologically relevant than traditional monolayer cultures. Cancers such as ovarian (OvCa) prone to developing resistance, often lethal, stand benefit greatly from enhanced modelling emulated by 3D However, current fall short predicted response, where reproducibility limited owing lack standardised methodology...
Abstract Assessing cell morphology and function, as well biomaterial performance in cultures, is one of the key challenges biology tissue engineering (TE) research. In TE, there an urgent need for methods to image actual three-dimensional (3D) cultures access living cells. This difficult using established optical microscopy techniques such wide-field or confocal microscopy. To address problem, we have developed a new protocol Optical Projection Tomography (OPT) extract quantitative...
This study focuses on improving the reconstruction process of brightfield optical projection tomography (OPT). OPT is often described as equivalent X-ray computed tomography, but based visible light. The detection optics used to collect light in focus a certain distance and induce blurring those features out focus. However, conventionally inverse Radon transform assumes an absolute throughout propagation axis. In this study, we model focusing properties by coupling Gaussian beam (GBM) with...
Solving the fluorophore distribution in a tomographic setting has been difficult because of lack physically meaningful and computationally applicable propagation models. This study concentrates on direct modelling fluorescence signals optical projection tomography (OPT), corresponding inverse problem. The reconstruction problem is solved using emission projections to series rotational imaging positions sample. Similarly bright field OPT bearing resemblance with transmission x-ray computed...
We present a computational model describing the blurring of particles with respect to focal distance in 3D optical imaging. The can be used improve reconstructions projection tomography.
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM) models have become an attractive tool for in vitro cardiac disease modeling and drug studies. These are moving towards more complex three-dimensional microphysiological organ-on-chip systems. Label-free imaging-based techniques capable of quantifying contractility 3D needed, as traditional two-dimensional methods ill-suited applications. Here, we developed multifocal (MF) optical projection microscopy (OPM) by integrating...
Abstract Three-dimensional (3D) cancer models are revolutionizing research, allowing for the recapitulation of in vivo like response through use an vitro system, more complex and physiologically relevant than traditional mono-layer culture. Cancers such as ovarian (OvCa), prone to developing resistance often lethal, stand benefit greatly from enhanced modelling emulated by 3D However current fall short predicted where reproducibility is limited owing lack standardized methodology established...