A5021760969- 3D Printing in Biomedical Research
- Collagen: Extraction and Characterization
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- Tendon Structure and Treatment
- South Asian Cinema and Culture
- Electrospun Nanofibers in Biomedical Applications
- South Asian Studies and Diaspora
- Optical Imaging and Spectroscopy Techniques
- Silk-based biomaterials and applications
- South Asian Studies and Conflicts
- Sports injuries and prevention
- Photoacoustic and Ultrasonic Imaging
Tampere University
2016-2019
Tampere University Hospital
2016-2019
Growing number of musculoskeletal defects increases the demand for engineered tendon. Our aim was to find an efficient strategy produce tendon-like matrix in vitro. To allow differentiation human adipose stem cells (hASCs) toward tendon tissue, we tested different medium compositions, biomaterials, and scaffold structures preliminary tests. This is first study report that supplementation with 50 ng/mL growth factor-5 (GDF-5) 280 μM l-ascorbic acid are essential tenogenic hASCs. Tenogenic...
Abstract Lack of bone grafts appeals for augmentation solutions. We aimed at osteogenic differentiation human adipose stem cells (hASCs) and microvascularization in coculture with umbilical vein endothelial (HUVECs) embedded three‐dimensional (3D) gellan gum (GG) collagen type I (COL) hydrogel mixture. compared growth medium‐2 (EGM‐2) bioactive glass extract‐based medium (BaG EM‐OM) vascularized bone‐like graft development vitro . Cell viability, cell number, gene expression were analyzed....
This article proposes the coupling of recombinant protein avidin to polysaccharide gellan gum create a modular hydrogel substrate for 3D cell culture and tissue engineering. Avidin is capable binding biotin, thus biotinylated compounds can be tethered polymer network improve response. The successfully conjugated remains functional as shown with fluorescence titration electrophoresis (SDS-PAGE). Self-standing hydrogels were formed using bioamines calcium chloride, yielding long-term stability...