A5018786715- Laser-induced spectroscopy and plasma
- Cultural Heritage Materials Analysis
- Analytical chemistry methods development
- Spectroscopy Techniques in Biomedical and Chemical Research
- Light effects on plants
- Spectroscopy and Chemometric Analyses
- Mass Spectrometry Techniques and Applications
- Photoreceptor and optogenetics research
- Microbial Community Ecology and Physiology
- Surface Roughness and Optical Measurements
- Photosynthetic Processes and Mechanisms
- Mercury impact and mitigation studies
- Bone Tissue Engineering Materials
- Geological and Geochemical Analysis
- High-pressure geophysics and materials
- Material Properties and Processing
- Biosensors and Analytical Detection
- Luminescence Properties of Advanced Materials
- Mineralogy and Gemology Studies
- Mineral Processing and Grinding
- Microbial Natural Products and Biosynthesis
- Diamond and Carbon-based Materials Research
- Bone health and osteoporosis research
- Plant and Biological Electrophysiology Studies
- Gas Sensing Nanomaterials and Sensors
University of Jyväskylä
2014-2024
Center for NanoScience
2017-2021
University of Gothenburg
2016
Paul Scherrer Institute
2016
Phytochrome proteins regulate many photoresponses of plants and microorganisms. Light absorption causes isomerization the biliverdin chromophore, which triggers a series structural changes to activate signaling domains protein. However, are elusive, therefore molecular mechanism signal transduction remains poorly understood. Here, we apply two-color step-scan infrared spectroscopy bacteriophytochrome from Deinococcus radiodurans. We show by recordings in H2O D2O that hydrogen bonds D-ring...
Three new epithiodiketopiperazine natural products [outovirin A (1), outovirin B (2), and C (3)] resembling the antifungal product gliovirin have been identified in extracts of Penicillium raciborskii, an endophytic fungus isolated from Rhododendron tomentosum. The compounds are unusual for their class that they possess sulfide bridges between α- β-carbons rather than typical α–α bridging. To our knowledge, represents first reported naturally produced epimonothiodiketopiperazine, is...
In this work we investigate the relationship of F line and CaF bands with varying Ca contents.
Phytochromes sense red light in plants and various microorganism. Light absorption causes structural changes within the protein, which alter its biochemical activity. Bacterial phytochromes are dimeric proteins, but functional relevance of this arrangement remains unclear. Here, we use time-resolved X-ray scattering to reveal solution change a monomeric variant photosensory core module phytochrome from Deinococcus radiodurans. The data two motions, bend twist PHY domain with respect...
The potential of a laser-generated plasma method in the analysis coating coverage, coatweight distribution, and 3D distribution various pigments paper is described. A XeCl-excimer laser (308 nm) was used to generate microscopic from coating, delayed detection silicon calcium atomic emission line intensities as measure mass vaporized. Macroscopic areas typically 10 × mm 2 at spatial resolution 250 μm were studied. With single pulse (0.2 mJ energy), about ng volume 30 diameter depth seems be...
Vibrational spectroscopy and crystallography experiments provide a basis for understanding the isomerization reaction in phytochrome proteins.
ABSTRACT In recent decades, Raman spectroscopy has entered the biological and medical fields. It enables nondestructive analysis of structural details at molecular level been used to study viruses their constituents. Here, we echovirus 1 (EV1), a small, nonenveloped human pathogen, in two different uncoating states induced by heat treatments. signals capsid proteins RNA genome were observed from intact virus, intermediate, disrupted virions. Transmission electron microscopy data revealed...
Abstract Raman analysis of rock samples containing rare earth elements (REEs) is challenging due to the strong fluorescence, which may mask weaker signal. In this research, time‐gated (TG) has been applied construction mineral distribution map from REE‐bearing rock. With TG Raman, material excited with a short subnanosecond laser pulse, and signal collected within picosecond‐scale time window prior formation fluorescent by means single‐photon avalanche diode array. This allows readout...
In addition to oxygenic photosynthetic systems, solar radiation is utilized for energy by diverse anoxygenic systems. Aerobic phototrophic bacteria (AAPB) perform photosynthesis without producing oxygen but still live in aerobic conditions. Typically they have been reported from aquatic ecosystems, can also be found polar and desert soil ecosystems primary succession communities like crusts. Recently, AAPB discovered the metagenomic data of several plant phyllospheres. By utilizing citizen...
Laser-induced plasma emission spectroscopy (LIPS) has been used to study inorganic pigment and organic binder distributions in paper coatings, which are inhomogeneous porous materials. The was generated by focusing a pulsed XeCl excimer laser beam (diameter 100 μm, irradiance 0.3 GW/cm2) on the sample surface at atmospheric pressure. A gated intensified CCD detector record time-delayed spectra. Linear correlations between LIPS signals coat weight content of coatings studied were obtained....
The optically active defects in as-grown, high-pressure high-temperature-treated (HPHT), borondoped, and synthetic diamonds (SD) grown with a nitrogen-getter, as well of natural (ND), were characterized by absorption luminescence spectroscopies using different excitation sources. laser-excited photoluminescence (PL) spectra SDs show numerous sharp lines characteristic for nickel-related centers, whereas NDs yield mainly broad PL bands. emission from the NIR range increases maxima bands shift...
Several solid organic compounds containing bromine and chlorine were analyzed with laser-induced plasma spectroscopy. Emission lines detected in the near vacuum ultraviolet spectral region by using a gas-purged spectrograph an intensified charge-coupled device detector. The performance of this setup determination halides samples was evaluated. Carbon emission used as internal standards for measurement bromine. Linear correlation found between carbon halogen signal ratio corresponding atomic compound.
Vibrationally distinguishable amino acid, azidophenylalanine, reveals site-specific information of a bacteriophytochrome in action. Collective responses are observed, yet each site shows specific kinetics the protein signal transduction.
193 nm excited time gated emission spectra of a NO monomer isolated in Ar, Kr, and Xe matrices are presented. In the Ar matrix 4Π→X 2Π, B 2Π→X A 2Σ→X 2Π band systems completely separable. solid both appear promptly from laser pulse, only Rydberg fluorescence is observed. Prolonged photolysis at yields electron paramagnetic resonance signals attributed to S4 nitrogen atoms. This first observation condensed phase photodissociation NO. Annealing extensively irradiated produces strong...
Mapping with laser-induced breakdown spectroscopy (LIBS) can offer more than just the spatial distribution of elements: rich spectral information also enables mineral recognition. In present study, statistical approaches were used for recognition spodumene from lithium pegmatite ores. A broad range (280–820 nm) multiple lines was first to establish methods based on vertex component analysis (VCA) and K-means DBSCAN clusterings. However, a view potential on-site applications, dimensions...
A novel method was developed for studying the ink-paper interface and structural variations of a deposited layer ink. Combining high-resolution x-ray tomography with laser ablation, depth profile ink (toner), i.e., its varying thickness, could be determined in paper substrate. X-ray used to produce 3D structure about 1 μm spatial resolution. Laser ablation combined optical imaging printed on top that 70 nm Ablation calibrated an profilometer. It can concluded toner light-weight-coated...
An experimental setup is described for measuring laser-induced plasma emission spectra in the near vacuum UV with a Czerny-Turner spectrograph and intensified charge-coupled device under atmospheric pressure. With simple gas-purge technique, lines down to 130 nm could be recorded. The strongest of bromine, chlorine, iodine are easily detected.