A5012552968- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Orthopaedic implants and arthroplasty
- Dental Implant Techniques and Outcomes
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Oral microbiology and periodontitis research
- Bone Metabolism and Diseases
- Advanced Cellulose Research Studies
- Dental materials and restorations
- Endodontics and Root Canal Treatments
- Bone and Dental Protein Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Wound Healing and Treatments
- Pluripotent Stem Cells Research
- Angiogenesis and VEGF in Cancer
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Diamond and Carbon-based Materials Research
- Genetic and phenotypic traits in livestock
- Protease and Inhibitor Mechanisms
University of Bergen
2016-2025
Alberta Health Services
2024
University of Al Maarif
2024
University of Duhok
2022-2023
Haukeland University Hospital
2023
Medical University of Vienna
2023
University of Bern
2023
Bergen Kommune
2019
Malmö University
2017
University of Wisconsin–Madison
2011
Adipose-derived stem cells (ASCs) have been introduced as an alternative to bone marrow mesenchymal (BMSCs) for cell-based therapy. However, different studies comparing ASCs and BMSCs shown conflicting results. In fact, harvesting from individuals might influence the results, making comparison difficult. Therefore, this study aimed characterize donor-matched in order investigate proliferation, differentiation potential possible effects of donor variation on these (MSCs).Human adipose tissue...
Abstract Background and Aims To review the regenerative technologies used in bone regeneration: grafts, barrier membranes, bioactive factors cell therapies. Material Methods Four background publications served to elaborate this consensus report. Results Conclusions Biomaterials as grafts must meet specific requirements: biocompatibility, porosity, osteoconductivity, osteoinductivity, surface properties, biodegradability, mechanical angiogenicity, handling manufacturing processes. Currently...
Autologous grafting, despite some disadvantages, is still considered the gold standard for reconstruction of maxillofacial bone defects. The aim this study was to evaluate regeneration using marrow-derived mesenchymal stromal cells (MSCs) in a clinical trial, less invasive approach than autologous grafting. This comprehensive trial included subjects with severe mandibular ridge resorption. 11 aged 52–79 years Bone marrow were aspirated from posterior iliac crest and plastic adherent expanded...
A challenge in the extrusion-based bioprinting is to find a bioink with optimal biological and physicochemical properties. The aim of this study was evaluate influence wood-based cellulose nanofibrils (CNF) bioactive glass (BaG) on rheological properties gelatin–alginate bioinks initial responses bone cells embedded these inks. CNF modulated flow behavior hydrogels, thus improving their printability. Chemical characterization by SEM-EDX ion release analysis confirmed reactivity BaG...
Abstract: In the complex process of bone formation at implant‐tissue interface, implant surface roughness is an important factor modulating osteoblastic function. this study, primary cultures osteoblast‐like cells, derived from human mandibular bone, were used. The aim was to examine effect varying titanium material on cellular attachment, proliferation and differentiation. A recognized method increasing enlarging area implants by blasting with dioxide particles: four specimen types in study...
Abstract Aim To assess ex vivo the cytotoxic effects of five new root canal sealers (RC Sealer, Epiphany, EndoREZ, GuttaFlow and Acroseal) three existing products (AH Plus, RoekoSeal Apexit) using primary human gingival fibroblasts (HGF) a mouse fibroblast cell line, L929. Methodology Eight samples each sealer were fabricated in sterile cylindrical Teflon blocks, 4.4 mm diameter 2 height then divided into two groups, fresh aged specimens. Extraction specimens was carried out after setting...
3D printed polycaprolactone (PCL) has potential as a scaffold for bone tissue engineering, but the hydrophobic surface may hinder optimal cell responses. The properties can be improved by coating with cellulose nanofibrils material (CNF), multiscale hydrophilic biocompatible biomaterial derived from wood. In this study, human marrow-derived mesenchymal stem cells were cultured on culture plates (TCP) and PCL scaffolds coated CNF. Cellular responses to surfaces (viability, attachment,...
There is growing evidence that extracellular vesicles (EVs) play a crucial role in the paracrine mechanisms of transplanted human mesenchymal stem cells (hMSCs). Little known, however, about influence microenvironmental stimuli on osteogenic effects EVs. This study aimed to investigate properties and functions EVs derived from undifferentiated hMSC (Naïve-EVs) during early stage osteogenesis (Osteo-EVs). A further aim was assess osteoinductive potential Osteo-EVs for bone regeneration rat...
Wood-based nanocellulose is emerging as a promising nanomaterial in the field of tissue engineering due to its unique properties and versatile applications. Previously, we used TEMPO-mediated oxidation (TO) carboxymethylation (CM) chemical pretreatments prior mechanical fibrillation wood-based cellulose nanofibrils (CNFs) produce scaffolds with different surface chemistries. The aim current study was evaluate effects these on serum protein adsorption 2D 3D configurations TO-CNF CM-CNF then...
The current study aims to demonstrate the influence of surface chemistry wood-derived cellulose nanofibril (CNF) hydrogels on fibroblasts for tissue engineering applications. TEMPO-mediated oxidation or carboxymethylation pretreatments were employed produce with different chemistry. This demonstrates following: first, gelation CNF cell culture medium and formation stable improved rheological properties; second, response mouse cultured sandwiched within materials respect cytotoxicity,...
Abstract Wood-derived TEMPO-oxidized cellulose nanofibrils (CNFs) have potential as scaffolding for bone tissue engineering. Although biocompatible, the material lacks osteoconductive and appropriate mechanical properties. Incorporation of nano-hydroxyapatite (nHA) modification scaffold preparation methods could improve applicability. In this study, freeze-dried porous scaffolds were prepared using a range nHA (0, 20, 33, 50%) CNF compositions. Not only microarchitecture but also chemical...
Abstract Background Improved understanding of the interactions between bone cells and endothelial involved in osteogenesis should aid development new strategies for tissue engineering. The aim present study was to determine whether direct communication marrow stromal (MSC) human umbilical vein (EC) could influence osteogenic potential MSC factor-free medium. Methods After adding EC a direct-contact system, cell viability morphology were investigated with WST assay immnostaining. effects on...
Nanostructured calcium phosphate (CaP) has been histologically and biomechanically proven to enhance osseointegration of implants; however, conventional techniques were not sufficiently sensitive capture its biological effects fully. Here, we compared the removal torque (RTQ) evaluation gene expression in tissues around nanostructured CaP-coated implants, using real-time RT-PCR, with those uncoated a rabbit model. At 2 wks, RTQ values significantly higher, alkaline phosphatase (ALP) was...
Abstract Introduction Rapid establishment of functional blood vessels is a prerequisite for successful tissue engineering. During vascular development, endothelial cells (ECs) and perivascular assemble into complex regulating proliferation ECs, vessel diameter production extracellular matrix proteins. The aim this study was to evaluate the ability mesenchymal stem (MSCs) establish an endothelial-perivascular in tissue-engineered constructs comprising ECs MSCs. Methods Primary human MSCs were...
Aim The purpose of this study was to compare the clinical and subjective oral health indicators type 2 diabetic patients (T2DM) with age gender matched non-diabetic controls. A second aim identify that discriminate between well-controlled poorly controlled T2DM as well long short duration disease. Methods total 457 individuals participated in (154 cases 303 controls). group sub-divided according metabolic control [(well-controlled: glycosylated haemoglobin test ≤8%), (poorly controlled: >...
Resolution agonists are endogenous mediators that drive inflammation to homeostasis. We earlier demonstrated in vivo activity of resolvins and lipoxins on regenerative periodontal wound healing. The goal this study was determine the impact resolvin D1 (RvD1) function human ligament (PDL) fibroblasts, which critical for healing during regeneration soft hard tissues around teeth. Primary cells were cultured from biopsies obtained three individuals free diseases. Peripheral blood mononuclear...
Significant evidence has indicated that poly(L-lactide)-co-(ɛ-caprolactone) [(poly(LLA-co-CL)] scaffolds could be one of the suitable candidates for bone tissue engineering. Oxygen-terminated nanodiamond particles (n-DP) were combined with poly(LLA-co-CL) and revealed to positive cell growth. In this study, we evaluated influence modified by n-DP on attachment, proliferation, differentiation marrow stromal cells (BMSCs) in vitro, formation using a sheep calvarial defect model. BMSCs seeded...